BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0132300 Os02g0132300|AK106973
         (272 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os02g0132300  Similar to Erythrocyte membrane protein PFEMP3...   563   e-161
Os01g0610700  RINGv domain containing protein                     162   2e-40
Os08g0384900  RINGv domain containing protein                     135   2e-32
Os11g0604600  RINGv domain containing protein                     129   3e-30

>Os02g0132300 Similar to Erythrocyte membrane protein PFEMP3 (Fragment)
          Length = 272

 Score =  563 bits (1450), Expect = e-161,   Method: Compositional matrix adjust.
 Identities = 272/272 (100%), Positives = 272/272 (100%)

Query: 1   MISVLFIMQLASHATQADEATELASSCDCAEVPASQQIVSESSTAGSSTEHLVSCEIKPL 60
           MISVLFIMQLASHATQADEATELASSCDCAEVPASQQIVSESSTAGSSTEHLVSCEIKPL
Sbjct: 1   MISVLFIMQLASHATQADEATELASSCDCAEVPASQQIVSESSTAGSSTEHLVSCEIKPL 60

Query: 61  GVDEDIETIDANEETHLVIQDCPQCRICLDNEGDDLIAPCHCKGTQKYVHRSCLDNWRST 120
           GVDEDIETIDANEETHLVIQDCPQCRICLDNEGDDLIAPCHCKGTQKYVHRSCLDNWRST
Sbjct: 61  GVDEDIETIDANEETHLVIQDCPQCRICLDNEGDDLIAPCHCKGTQKYVHRSCLDNWRST 120

Query: 121 KEGFAFSHCTECRAAFLLRANVPPDRWWLRLKFQLLVVRDHTLIFFIVQLVVALLGMLVY 180
           KEGFAFSHCTECRAAFLLRANVPPDRWWLRLKFQLLVVRDHTLIFFIVQLVVALLGMLVY
Sbjct: 121 KEGFAFSHCTECRAAFLLRANVPPDRWWLRLKFQLLVVRDHTLIFFIVQLVVALLGMLVY 180

Query: 181 RFYGDELREMFGYEEHPYAFYAMAILAIVLVGLLYGFFIAIICGQRITERHYHVLAKQEL 240
           RFYGDELREMFGYEEHPYAFYAMAILAIVLVGLLYGFFIAIICGQRITERHYHVLAKQEL
Sbjct: 181 RFYGDELREMFGYEEHPYAFYAMAILAIVLVGLLYGFFIAIICGQRITERHYHVLAKQEL 240

Query: 241 TKEYIVEDLEGTDGVPDLDPNHVTELRILGLY 272
           TKEYIVEDLEGTDGVPDLDPNHVTELRILGLY
Sbjct: 241 TKEYIVEDLEGTDGVPDLDPNHVTELRILGLY 272



>Os01g0610700 RINGv domain containing protein
          Length = 309

 Score =  162 bits (411), Expect = 2e-40,   Method: Compositional matrix adjust.
 Identities = 100/254 (39%), Positives = 126/254 (49%), Gaps = 67/254 (26%)

Query: 85  CRICLDNE---GDDLIAPCHCKGTQKYVHRSCLDNWRSTKEGFAFSHCTECRAAFLLRAN 141
           CRICL++E   GD+LI+PC CKGTQ++VHR CLD+WRS KEG AFSHCT C+A F LR  
Sbjct: 55  CRICLESETEPGDELISPCMCKGTQQFVHRYCLDHWRSVKEGTAFSHCTTCKAQFHLRVE 114

Query: 142 VPPDRWWLRLKFQLLVVRDHTLIFFIVQLVVALLGMLVYRFYGD-ELREMF--GYE---- 194
              D    ++ F+L V RD  L+F  VQ V+A +G + Y    D + R  F  G+E    
Sbjct: 115 CLEDNLCRKMMFRLFVARDVFLVFLAVQTVIAAIGGMAYLLDKDGQFRNSFSDGWEHILS 174

Query: 195 EHPYAFY---------------------------------------------------AM 203
            HP  FY                                                   A 
Sbjct: 175 NHPVPFYYCVGVVVFFVMVGFIGLIVQCSSFNTNDPCLAGCRNCCYGWGILDFPASIEAC 234

Query: 204 AILAIVLV------GLLYGFFIAIICGQRITERHYHVLAKQELTKEYIVEDLEGTDGVPD 257
             LA++ V      G+ YGFF A +  QRI +RHYH+L K+ELTKEY+VEDL G    P 
Sbjct: 235 LALAVIFVIVFAILGVAYGFFAATVAVQRILQRHYHILTKRELTKEYVVEDLRGCYTPPK 294

Query: 258 LDPNHVTELRILGL 271
           +DP     LR L L
Sbjct: 295 MDPEQEQRLRTLQL 308



>Os08g0384900 RINGv domain containing protein
          Length = 314

 Score =  135 bits (341), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 74/161 (45%), Positives = 100/161 (62%), Gaps = 14/161 (8%)

Query: 72  NEETHLVIQDCPQCRICLDNE---GDDLIAPCHCKGTQKYVHRSCLDNWRSTKEGFAFSH 128
           +EET      C  CRICL+ +   GD+LI+PC CKGTQ++VHRSCLD+WRS KEGFAFSH
Sbjct: 47  DEETDGSSAGC--CRICLETDSELGDELISPCMCKGTQQFVHRSCLDHWRSVKEGFAFSH 104

Query: 129 CTECRAAFLLRANVPPDRWWLRLKFQLLVVRDHTLIFFIVQLVVALLGMLVYRFYGD-EL 187
           CT C+A F LR     D  W ++KF++ V RD  L+F  VQL +A++G + Y    D   
Sbjct: 105 CTTCKAQFHLRVETWEDNSWRKMKFRIFVARDVILVFLAVQLTIAMIGAISYFLDRDGSF 164

Query: 188 REMF--GYE----EHPYAFYAMAILAIVLVGLLYGFFIAII 222
           R  F  G++    +HP  FY    + +V+  +L GFF  I+
Sbjct: 165 RNSFSDGWDRFLSKHPIPFY--YCIGVVVFFVLLGFFGLIL 203




 Score = 74.7 bits (182), Expect = 6e-14,   Method: Compositional matrix adjust.
 Identities = 34/74 (45%), Positives = 49/74 (66%)

Query: 198 YAFYAMAILAIVLVGLLYGFFIAIICGQRITERHYHVLAKQELTKEYIVEDLEGTDGVPD 257
           +A   + ++   ++G+ YGF  A +  QRI +RHYH+L K+ELTKEY+VEDL G+   P 
Sbjct: 240 FALVVVFVVVFAILGIAYGFLAATMAVQRIWQRHYHILTKRELTKEYVVEDLHGSYTPPK 299

Query: 258 LDPNHVTELRILGL 271
           LDP H   L++L L
Sbjct: 300 LDPEHEERLKMLKL 313



>Os11g0604600 RINGv domain containing protein
          Length = 325

 Score =  129 bits (324), Expect = 3e-30,   Method: Compositional matrix adjust.
 Identities = 67/139 (48%), Positives = 85/139 (61%), Gaps = 3/139 (2%)

Query: 84  QCRICLDNEGDDLIAPCHCKGTQKYVHRSCLDNWRSTKEGFAFSHCTECRAAFLLRANVP 143
           QCRICL+ +G D IAPC CKGT KYVHR CLD+WR+ KEGFAFSHCT C+A + LR +V 
Sbjct: 37  QCRICLETDGRDFIAPCKCKGTSKYVHRDCLDHWRAVKEGFAFSHCTTCKAPYYLRVHVH 96

Query: 144 PDRWWLRLKFQLLVVRDHTLIFFIVQLVVALLGMLVYRFYGDE---LREMFGYEEHPYAF 200
            DR W  LKF+  V RD   IF +VQ V++ L  LV+   G +   LR  + ++     +
Sbjct: 97  TDRKWRTLKFRFFVTRDILFIFALVQFVISALAYLVHFIDGLQNYWLRTAWAFDNEVSFY 156

Query: 201 YAMAILAIVLVGLLYGFFI 219
           Y    L    +  L G FI
Sbjct: 157 YICGALLFFALLGLSGCFI 175




 Score = 65.9 bits (159), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 33/78 (42%), Positives = 49/78 (62%), Gaps = 6/78 (7%)

Query: 200 FYAMAILAIVL---VGLLYGFFIAIICGQRITERHYHVLAKQELTKEYIVEDLEGTDG-- 254
            + M ++ + L   +G+ Y   +A + GQRI +RHYH+LAK+ LTKEY+VED++G     
Sbjct: 247 LFIMGVIVLGLFTVIGIFYSVLVATMVGQRIWQRHYHILAKRMLTKEYVVEDVDGERADW 306

Query: 255 -VPDLDPNHVTELRILGL 271
             P L   HV +L+ LGL
Sbjct: 307 CPPPLPSEHVQQLKSLGL 324



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.139    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,060,446
Number of extensions: 361448
Number of successful extensions: 1232
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1232
Number of HSP's successfully gapped: 7
Length of query: 272
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 173
Effective length of database: 11,866,615
Effective search space: 2052924395
Effective search space used: 2052924395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 155 (64.3 bits)