BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0897300 Os01g0897300|AK061032
         (271 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os01g0897300  Ribonuclease T2 family protein                      568   e-162
Os01g0897200  Similar to Ribonuclease 2 precursor (EC 3.1.27.1)   279   1e-75
Os08g0434100  Similar to S-like ribonuclease (RNase PD2) (Fr...   112   2e-25
Os07g0630400  Ribonuclease T2 family protein                       96   2e-20
Os07g0629900                                                       70   1e-12

>Os01g0897300 Ribonuclease T2 family protein
          Length = 271

 Score =  568 bits (1464), Expect = e-162,   Method: Compositional matrix adjust.
 Identities = 271/271 (100%), Positives = 271/271 (100%)

Query: 1   MEEKRQRLVISLSALCLAVAVMSGFDYYVLALQWPGTVCRQTSHCCSSNGCCRSHPLKFF 60
           MEEKRQRLVISLSALCLAVAVMSGFDYYVLALQWPGTVCRQTSHCCSSNGCCRSHPLKFF
Sbjct: 1   MEEKRQRLVISLSALCLAVAVMSGFDYYVLALQWPGTVCRQTSHCCSSNGCCRSHPLKFF 60

Query: 61  TIHGLWPQYSYGGWPSCCRPTTFDGNKVAANHPSYLQNGILISRLKTILEEYWPSLYCGS 120
           TIHGLWPQYSYGGWPSCCRPTTFDGNKVAANHPSYLQNGILISRLKTILEEYWPSLYCGS
Sbjct: 61  TIHGLWPQYSYGGWPSCCRPTTFDGNKVAANHPSYLQNGILISRLKTILEEYWPSLYCGS 120

Query: 121 FSTCFGGKRPFWVHEWETHGTCGYPEIQDEYDYFSTALYLYSKYNVTKALKKAHIYPRGG 180
           FSTCFGGKRPFWVHEWETHGTCGYPEIQDEYDYFSTALYLYSKYNVTKALKKAHIYPRGG
Sbjct: 121 FSTCFGGKRPFWVHEWETHGTCGYPEIQDEYDYFSTALYLYSKYNVTKALKKAHIYPRGG 180

Query: 181 RKYLVGHIVSAIEFSFGAMPSIVCKNGSVQELRLCFHKDYQPRDCLVEGENSVRRNHCPR 240
           RKYLVGHIVSAIEFSFGAMPSIVCKNGSVQELRLCFHKDYQPRDCLVEGENSVRRNHCPR
Sbjct: 181 RKYLVGHIVSAIEFSFGAMPSIVCKNGSVQELRLCFHKDYQPRDCLVEGENSVRRNHCPR 240

Query: 241 YVTLPSYKPHAFGNSTEGISNQVNVEHQSYQ 271
           YVTLPSYKPHAFGNSTEGISNQVNVEHQSYQ
Sbjct: 241 YVTLPSYKPHAFGNSTEGISNQVNVEHQSYQ 271



>Os01g0897200 Similar to Ribonuclease 2 precursor (EC 3.1.27.1)
          Length = 284

 Score =  279 bits (714), Expect = 1e-75,   Method: Compositional matrix adjust.
 Identities = 133/249 (53%), Positives = 164/249 (65%), Gaps = 20/249 (8%)

Query: 25  FDYYVLALQWPGTVCRQTSHCCSSNGCCRSHPLKFFTIHGLWPQYSYGGWPSCCRPTTFD 84
           FDY+ LALQWPGT+C  T HCC+ NGCCRS PL+ FTIHGLWP Y  G WP+CCR T+FD
Sbjct: 47  FDYFALALQWPGTICASTRHCCAINGCCRSEPLQTFTIHGLWPDYDDGTWPACCRHTSFD 106

Query: 85  GNKVAANHPSYLQNGILISRLKTILEEYWPSLYCGSFSTCFGGKRPFWVHEWETHGTCGY 144
            +K+                LK  LE+YWPSLYC S STCF GK PFW HE E HGTC  
Sbjct: 107 MDKILP--------------LKPTLEKYWPSLYCSSSSTCFSGKGPFWAHESEKHGTCSS 152

Query: 145 PEIQDEYDYFSTALYLYSKYNVTKALKKAHIYPRGGRKYLVGHIVSAIEFSFGAMPSIVC 204
           P ++DE +YF+TAL LY KYNVT+ L    I+   G++Y +  ++ AI+ +FGA P IVC
Sbjct: 153 PVVKDELEYFTTALDLYFKYNVTEMLASGGIHVSNGKQYALTDVIDAIKCAFGASPQIVC 212

Query: 205 KNGSVQELRLCFHKDYQPRDCLV---EGENSVRRNHCPRYVTLPSYKPHAFGNSTEGISN 261
           K GSV+ELRLCF KD +P DCL      EN  ++ +CPRY+TLP+Y P    NST  I  
Sbjct: 213 KKGSVEELRLCFDKDLKPLDCLTTTATNENVSKKKYCPRYITLPTYDPIVHANSTREI-- 270

Query: 262 QVNVEHQSY 270
            + VE + Y
Sbjct: 271 -ITVESEVY 278



>Os08g0434100 Similar to S-like ribonuclease (RNase PD2) (Fragment)
          Length = 229

 Score =  112 bits (281), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 77/212 (36%), Positives = 99/212 (46%), Gaps = 29/212 (13%)

Query: 14  ALCLAVAVMSG--FDYYVLALQWPGTVCRQTSHCCSSNGCCRSHPLKFFTIHGLWPQYSY 71
           AL LAV  +    +D++ L LQWPG+ C     CC         P   F IHGLWP    
Sbjct: 14  ALLLAVVGVGAQDYDFFFLVLQWPGSYCDTKQSCCYPR---SGKPAADFGIHGLWPNRDD 70

Query: 72  GGWPSCCRPTT-FDGNKVAANHPSYLQNGILISRLKTILEEYWPSLYCGSFSTCFGGKRP 130
           G +P  C P + FD +KV              S L   +   WP+L C S      G R 
Sbjct: 71  GSYPQNCDPDSEFDPSKV--------------SDLLGSMRSEWPTLACPSND----GIR- 111

Query: 131 FWVHEWETHGTCGYPEIQDEYDYFSTALYLYSKYNVTKALKKAHIYPRGGRKYLVGHIVS 190
           FW HEWE HGTC    + DE+ YF  AL L S+  V  AL+   + P GG  Y +  I  
Sbjct: 112 FWAHEWEKHGTCAAAALGDEHGYFEAALRLRSRLPVLAALRDGGVSPDGG-YYTLSQIKG 170

Query: 191 AIEFSFGAMPSIVC---KNGSVQELRLCFHKD 219
           AI+   GA P + C   ++G+ Q  +L F  D
Sbjct: 171 AIQRGVGAEPFVECNRDESGNSQLYQLYFCVD 202



>Os07g0630400 Ribonuclease T2 family protein
          Length = 256

 Score = 96.3 bits (238), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 66/218 (30%), Positives = 92/218 (42%), Gaps = 46/218 (21%)

Query: 25  FDYYVLALQWPGTVCRQTSHCCSSNGCCRSHPLKFFTIHGLWPQYSYGGWPSCCRP---- 80
           FD++ L  QWPG+ C   + CC  +      P   F IHGLWP Y+       CRP    
Sbjct: 23  FDFFYLVQQWPGSFCDTQAGCCFPD---TGKPAAEFGIHGLWPNYAK------CRPAAGA 73

Query: 81  ----------TTFDGNKVAANH--------PSYLQNGIL-----ISRLKTILEEYWPSLY 117
                        DG      H        P Y  +G       I  L   L+  WP+L 
Sbjct: 74  VADDDDGVVEMVVDGGAAVERHHRRRQKCWPEYCDDGNKLRPWEIKDLVAELDANWPTL- 132

Query: 118 CGSFSTCFGGKR-PFWVHEWETHGTCGYPEIQDEYDYFSTALYLYSKYNVTKALKKAHIY 176
                +C GGK   FW +EW+ HGTC       ++ YF+ AL L  ++++   L  A I 
Sbjct: 133 -----SCKGGKSFEFWSYEWKKHGTCSG---MGQHGYFAAALELKKRHDLAAVLAGAGIV 184

Query: 177 PRGGRKYLVGHIVSAIEFSFGAMPSIVCKNGSVQELRL 214
           P     Y +G I  AI  + GA+P++ C   +  E +L
Sbjct: 185 PSDDESYSLGSIRDAIAAATGAVPNLECNRDAAGETQL 222



>Os07g0629900 
          Length = 260

 Score = 70.1 bits (170), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 61/208 (29%), Positives = 86/208 (41%), Gaps = 39/208 (18%)

Query: 20  AVMSGFDYYVLALQWPGTVCRQTSHCCSSNGCCRSHPLKFFTIHGLWPQYSY----GGWP 75
           +V   FD+  LA QWP + C  ++H C      +  P   FTIHGLWP Y+       W 
Sbjct: 22  SVAEEFDFMYLAQQWPDSYC--STHKC----LVKPPPPSHFTIHGLWPSYNKLIDGKMWL 75

Query: 76  SCCRPTTFDGNKVAANHPSYLQNGILISRLKTILEEYWPSLYCGSFSTCFGGKRPFWVHE 135
             C       NK     P+ +Q+      L+  L++ WPSL   +          FW  E
Sbjct: 76  EDC-------NKEDPLDPTQIQD------LEKQLDQKWPSLKQTNLE--------FWSLE 114

Query: 136 WETHGTCGYPEIQDEYDYFSTALYLYSKYNVTKALKKAHIYPRGGRKYLVGHIVSAIEFS 195
           W+ HGTC       ++ YF  AL L    N+TK L    + P   + Y    I  A+   
Sbjct: 115 WKKHGTCSN---LGQHAYFEAALALERLTNLTKILADGGVGPSDVKTYTFREISDALARG 171

Query: 196 FGAMPSIVC-KNGS----VQELRLCFHK 218
            G      C KN +    + E+R C  +
Sbjct: 172 TGFRTYFKCSKNKAGDTLLSEVRQCVDR 199



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.138    0.463 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,294,106
Number of extensions: 446642
Number of successful extensions: 761
Number of sequences better than 1.0e-10: 8
Number of HSP's gapped: 753
Number of HSP's successfully gapped: 8
Length of query: 271
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 172
Effective length of database: 11,866,615
Effective search space: 2041057780
Effective search space used: 2041057780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 155 (64.3 bits)