BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0621300 Os01g0621300|J075014C24
(78 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os01g0621300 Conserved hypothetical protein 161 8e-41
Os05g0586400 Conserved hypothetical protein 131 1e-31
Os02g0258300 Zinc finger, FYVE/PHD-type domain containing p... 112 8e-26
Os06g0524300 Hypothetical protein 103 2e-23
>Os01g0621300 Conserved hypothetical protein
Length = 78
Score = 161 bits (408), Expect = 8e-41, Method: Compositional matrix adjust.
Identities = 78/78 (100%), Positives = 78/78 (100%)
Query: 1 NAQPFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNI 60
NAQPFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNI
Sbjct: 1 NAQPFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNI 60
Query: 61 TDEETGKKLRSLGKLAPT 78
TDEETGKKLRSLGKLAPT
Sbjct: 61 TDEETGKKLRSLGKLAPT 78
>Os05g0586400 Conserved hypothetical protein
Length = 780
Score = 131 bits (329), Expect = 1e-31, Method: Compositional matrix adjust.
Identities = 59/75 (78%), Positives = 66/75 (88%)
Query: 4 PFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNITDE 63
PFI+EFNYPM NCA G++GV VNGRELHQ+D +LL GRGLPRISGKSYSVEI+GN+ DE
Sbjct: 689 PFIKEFNYPMPKNCAGGNTGVIVNGRELHQKDFELLAGRGLPRISGKSYSVEINGNVIDE 748
Query: 64 ETGKKLRSLGKLAPT 78
TGKKLR LGKLAPT
Sbjct: 749 TTGKKLRKLGKLAPT 763
>Os02g0258300 Zinc finger, FYVE/PHD-type domain containing protein
Length = 842
Score = 112 bits (279), Expect = 8e-26, Method: Compositional matrix adjust.
Identities = 50/75 (66%), Positives = 62/75 (82%)
Query: 4 PFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNITDE 63
P+I E NYPM CA+G++GVFVNGRELHQ+DLDLLVGRGLP G+SY VE+SG ++DE
Sbjct: 751 PYIPELNYPMPKKCAAGNTGVFVNGRELHQKDLDLLVGRGLPDSPGRSYRVEMSGKVSDE 810
Query: 64 ETGKKLRSLGKLAPT 78
+G++L LGKLAPT
Sbjct: 811 VSGEELYCLGKLAPT 825
>Os06g0524300 Hypothetical protein
Length = 811
Score = 103 bits (258), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 47/75 (62%), Positives = 59/75 (78%)
Query: 4 PFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNITDE 63
P+I EFNYPM NC G++G+F+NGRELHQ+DLDLLV RGL G+SY VE SG ++DE
Sbjct: 720 PYIPEFNYPMPKNCGGGNTGIFINGRELHQKDLDLLVSRGLSDSPGRSYIVENSGKVSDE 779
Query: 64 ETGKKLRSLGKLAPT 78
+G++L LGKLAPT
Sbjct: 780 VSGEELYGLGKLAPT 794
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.315 0.136 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,746,792
Number of extensions: 102523
Number of successful extensions: 121
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 121
Number of HSP's successfully gapped: 4
Length of query: 78
Length of database: 17,035,801
Length adjustment: 49
Effective length of query: 29
Effective length of database: 14,477,315
Effective search space: 419842135
Effective search space used: 419842135
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)