BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0600200 Os12g0600200|AK122147
         (394 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0600200  Conserved hypothetical protein                      677   0.0  
Os03g0798200  Zinc finger, RING-type domain containing protein    122   4e-28
Os03g0632800  Zinc finger, RING-type domain containing protein     70   4e-12

>Os12g0600200 Conserved hypothetical protein
          Length = 394

 Score =  677 bits (1748), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 339/394 (86%), Positives = 339/394 (86%)

Query: 1   MKYAPIYLLTLSCLHYAVCRKCIYKKINDEELEHCPVCKIDLGCAPLEKLRADHNIQDVR 60
           MKYAPIYLLTLSCLHYAVCRKCIYKKINDEELEHCPVCKIDLGCAPLEKLRADHNIQDVR
Sbjct: 1   MKYAPIYLLTLSCLHYAVCRKCIYKKINDEELEHCPVCKIDLGCAPLEKLRADHNIQDVR 60

Query: 61  SKIFPLKRKKVNAEEVESPIAPPAKRKERSISSLVVNTPEITPKSLTGXXXXXXXXXXXX 120
           SKIFPLKRKKVNAEEVESPIAPPAKRKERSISSLVVNTPEITPKSLTG            
Sbjct: 61  SKIFPLKRKKVNAEEVESPIAPPAKRKERSISSLVVNTPEITPKSLTGRRTRASTRKSAA 120

Query: 121 XLRDLGPIIPPVKKDSDNTNKNADNXXXXXXXXKVPQTRRQVLSNAETXXXXXXXXXXXX 180
            LRDLGPIIPPVKKDSDNTNKNADN        KVPQTRRQVLSNAET            
Sbjct: 121 ALRDLGPIIPPVKKDSDNTNKNADNSSLLDSLSKVPQTRRQVLSNAETSSHPSSKDKGGD 180

Query: 181 XXXXXXXELWRPLNCLVEAASKTKSYRSSSAARGNQPTESPSSANASRTKAREYLLKSKV 240
                  ELWRPLNCLVEAASKTKSYRSSSAARGNQPTESPSSANASRTKAREYLLKSKV
Sbjct: 181 DKDLDKSELWRPLNCLVEAASKTKSYRSSSAARGNQPTESPSSANASRTKAREYLLKSKV 240

Query: 241 QDEKKEVPVATVPFKRKGPGRGRKXXXXXXXXXXXXXXXKHEKLLTPVWFSLIASFDQKG 300
           QDEKKEVPVATVPFKRKGPGRGRK               KHEKLLTPVWFSLIASFDQKG
Sbjct: 241 QDEKKEVPVATVPFKRKGPGRGRKPAQPPAAAVSSHSASKHEKLLTPVWFSLIASFDQKG 300

Query: 301 APPLPQIPTHYLRIKDDNMPASSIQKYIMQKLSLPSETEVEISCCGQPVNPIQPLRNLIE 360
           APPLPQIPTHYLRIKDDNMPASSIQKYIMQKLSLPSETEVEISCCGQPVNPIQPLRNLIE
Sbjct: 301 APPLPQIPTHYLRIKDDNMPASSIQKYIMQKLSLPSETEVEISCCGQPVNPIQPLRNLIE 360

Query: 361 RWLRFGPARTLQTVVGSSGGDYVMVISYGRPKAA 394
           RWLRFGPARTLQTVVGSSGGDYVMVISYGRPKAA
Sbjct: 361 RWLRFGPARTLQTVVGSSGGDYVMVISYGRPKAA 394



>Os03g0798200 Zinc finger, RING-type domain containing protein
          Length = 385

 Score =  122 bits (306), Expect = 4e-28,   Method: Compositional matrix adjust.
 Identities = 74/210 (35%), Positives = 106/210 (50%), Gaps = 27/210 (12%)

Query: 190 WRPLNCLVEAASKTKSYRSS---SAARGNQPTESPSSANASRTKAREYLLKSKVQDEKKE 246
           WRPL C ++     KS +SS   +    ++  ++   A AS+ KARE             
Sbjct: 175 WRPLGCQLKLGKDKKSLKSSVKDTNRTKSKSGDTDDGAPASKAKARE------------- 221

Query: 247 VPVATVPFKRKG-----PGRGRKXXXXXXXXXXXXXXXKHEKLLTPVWFSLIASFDQKGA 301
                 PF R G      GR +                  ++    +WF L+A+FDQ+G 
Sbjct: 222 ------PFTRYGRAAKRTGRKKLLMLKNKKKRFKAKQPSKKRRFRALWFYLLAAFDQRGV 275

Query: 302 PPLPQIPTHYLRIKDDNMPASSIQKYIMQKLSLPSETEVEISCCGQPVNPIQPLRNLIER 361
           P LPQ+P  YLRIKD ++PAS IQKY+ QKL+L SETEVE+ C G+ VN    L +L + 
Sbjct: 276 PTLPQLPAKYLRIKDVDLPASIIQKYLAQKLNLSSETEVEVLCGGKVVNQGMTLHDLADC 335

Query: 362 WLRFGPARTLQTVVGSSGGDYVMVISYGRP 391
           WL  GP   +++ VGS    +++ + Y RP
Sbjct: 336 WLEKGPKSRMRSSVGSPATGFMVTLFYRRP 365




 Score =  113 bits (282), Expect = 3e-25,   Method: Compositional matrix adjust.
 Identities = 52/90 (57%), Positives = 67/90 (74%), Gaps = 1/90 (1%)

Query: 13  CLHYAVCRKCIYKKINDEELEHCPVCKIDLGCAPLEKLRADHNIQDVRSKIFPLKRKKVN 72
           CLH   CRKCI+++  D+E   CP C IDLGCAPLEKLR DH++Q VRS+IFP KR+KV 
Sbjct: 50  CLH-TFCRKCIHEEFVDKESCCCPTCNIDLGCAPLEKLRVDHSMQFVRSRIFPFKRRKVE 108

Query: 73  AEEVESPIAPPAKRKERSISSLVVNTPEIT 102
             E+  P+A P KRKERS+SSL +  P+++
Sbjct: 109 NPEIICPVASPVKRKERSLSSLTIPAPQVS 138



>Os03g0632800 Zinc finger, RING-type domain containing protein
          Length = 203

 Score = 69.7 bits (169), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 33/44 (75%), Positives = 37/44 (84%), Gaps = 2/44 (4%)

Query: 13  CLHYAVCRKCIYKKINDEELEHCPVCKIDLGCAPLEKLRADHNI 56
           CLH   CRKCIY+K+NDEE+E CPVCKIDLGC P+EKLR D NI
Sbjct: 126 CLH-TFCRKCIYEKLNDEEVESCPVCKIDLGCTPVEKLR-DGNI 167



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.132    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 12,201,934
Number of extensions: 448210
Number of successful extensions: 1532
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1530
Number of HSP's successfully gapped: 4
Length of query: 394
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 291
Effective length of database: 11,657,759
Effective search space: 3392407869
Effective search space used: 3392407869
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 157 (65.1 bits)