BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0580300 Os12g0580300|AK102871
         (208 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0580300  Similar to TATA-binding protein TBP2                402   e-112
Os03g0657000  TATA-binding protein TBP2                           396   e-111
Os10g0432300  Similar to Transcription factor IID, TFIID (Fr...   367   e-102

>Os12g0580300 Similar to TATA-binding protein TBP2
          Length = 208

 Score =  402 bits (1032), Expect = e-112,   Method: Compositional matrix adjust.
 Identities = 195/195 (100%), Positives = 195/195 (100%)

Query: 14  LEGSEPVDLVKHPSGIIPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIR 73
           LEGSEPVDLVKHPSGIIPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIR
Sbjct: 14  LEGSEPVDLVKHPSGIIPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIR 73

Query: 74  EPKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGFAAKFKDFKIQNIVGSCDVK 133
           EPKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGFAAKFKDFKIQNIVGSCDVK
Sbjct: 74  EPKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGFAAKFKDFKIQNIVGSCDVK 133

Query: 134 FPIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAF 193
           FPIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAF
Sbjct: 134 FPIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAF 193

Query: 194 ENIYPVLTEFRKVQQ 208
           ENIYPVLTEFRKVQQ
Sbjct: 194 ENIYPVLTEFRKVQQ 208



>Os03g0657000 TATA-binding protein TBP2
          Length = 203

 Score =  396 bits (1018), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 193/195 (98%), Positives = 193/195 (98%)

Query: 14  LEGSEPVDLVKHPSGIIPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIR 73
           LEGSEPVDL KHPSGIIPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIR
Sbjct: 9   LEGSEPVDLAKHPSGIIPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIR 68

Query: 74  EPKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGFAAKFKDFKIQNIVGSCDVK 133
           EPKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGF AKFKDFKIQNIVGSCDVK
Sbjct: 69  EPKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGFPAKFKDFKIQNIVGSCDVK 128

Query: 134 FPIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAF 193
           FPIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAF
Sbjct: 129 FPIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAF 188

Query: 194 ENIYPVLTEFRKVQQ 208
           ENIYPVLTEFRKVQQ
Sbjct: 189 ENIYPVLTEFRKVQQ 203



>Os10g0432300 Similar to Transcription factor IID, TFIID (Fragment)
          Length = 223

 Score =  367 bits (943), Expect = e-102,   Method: Compositional matrix adjust.
 Identities = 175/194 (90%), Positives = 185/194 (95%)

Query: 15  EGSEPVDLVKHPSGIIPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIRE 74
           +G+EPVDLV+HPSGI+PTLQNIVSTVNLDC+LDLK IALQARNAEYNPKRFAAVIMRIR+
Sbjct: 29  DGAEPVDLVEHPSGIVPTLQNIVSTVNLDCRLDLKKIALQARNAEYNPKRFAAVIMRIRD 88

Query: 75  PKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGFAAKFKDFKIQNIVGSCDVKF 134
           PKTTALIFASGKMVCTGAKSE  SKLAARKYARI+QKLGF AKFKDFKIQNIVGSCDVKF
Sbjct: 89  PKTTALIFASGKMVCTGAKSEDHSKLAARKYARIVQKLGFPAKFKDFKIQNIVGSCDVKF 148

Query: 135 PIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAFE 194
           PIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAK R E Y AFE
Sbjct: 149 PIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKYRKEIYAAFE 208

Query: 195 NIYPVLTEFRKVQQ 208
           N++PVLTE+RK QQ
Sbjct: 209 NMFPVLTEYRKTQQ 222



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.137    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,593,516
Number of extensions: 195659
Number of successful extensions: 368
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 362
Number of HSP's successfully gapped: 3
Length of query: 208
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 112
Effective length of database: 12,023,257
Effective search space: 1346604784
Effective search space used: 1346604784
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 154 (63.9 bits)