BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0500700 Os12g0500700|AK073408
         (306 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0500700  Similar to Vacuolar sorting protein-like            621   e-178
Os11g0629200  Similar to Vacuolar sorting protein-like            539   e-153
Os03g0679100  Vacuolar protein sorting-associated protein 26...    93   2e-19

>Os12g0500700 Similar to Vacuolar sorting protein-like
          Length = 306

 Score =  621 bits (1602), Expect = e-178,   Method: Compositional matrix adjust.
 Identities = 306/306 (100%), Positives = 306/306 (100%)

Query: 1   MNYIVGAFKPPCDISIAFSDARSRKQIAVKNDNGRTVMVPVFQSLETISGEVSVVPVPGK 60
           MNYIVGAFKPPCDISIAFSDARSRKQIAVKNDNGRTVMVPVFQSLETISGEVSVVPVPGK
Sbjct: 1   MNYIVGAFKPPCDISIAFSDARSRKQIAVKNDNGRTVMVPVFQSLETISGEVSVVPVPGK 60

Query: 61  RIEHLGVKIELLGQIELYLERGKFYDFTSLVRELDIPGEIYEKKTYPFEFSTVEMPYESY 120
           RIEHLGVKIELLGQIELYLERGKFYDFTSLVRELDIPGEIYEKKTYPFEFSTVEMPYESY
Sbjct: 61  RIEHLGVKIELLGQIELYLERGKFYDFTSLVRELDIPGEIYEKKTYPFEFSTVEMPYESY 120

Query: 121 NGTNVRLRYILKVTISRNIMGMGSIVEFREFCVRNYSPAPTINNSIKMEVGIEDCLHIEF 180
           NGTNVRLRYILKVTISRNIMGMGSIVEFREFCVRNYSPAPTINNSIKMEVGIEDCLHIEF
Sbjct: 121 NGTNVRLRYILKVTISRNIMGMGSIVEFREFCVRNYSPAPTINNSIKMEVGIEDCLHIEF 180

Query: 181 EYSKSKYHLKDVIIGKIYFLLVRIKIKNMELEVRRRESTGAGSSAYVETETLAKFELMDG 240
           EYSKSKYHLKDVIIGKIYFLLVRIKIKNMELEVRRRESTGAGSSAYVETETLAKFELMDG
Sbjct: 181 EYSKSKYHLKDVIIGKIYFLLVRIKIKNMELEVRRRESTGAGSSAYVETETLAKFELMDG 240

Query: 241 APVRGESIPVRLFLTPYELTPSYRNINNKFSVKYYLNLVLVDEEDRRYFKQQEITMYRLL 300
           APVRGESIPVRLFLTPYELTPSYRNINNKFSVKYYLNLVLVDEEDRRYFKQQEITMYRLL
Sbjct: 241 APVRGESIPVRLFLTPYELTPSYRNINNKFSVKYYLNLVLVDEEDRRYFKQQEITMYRLL 300

Query: 301 ETPQAS 306
           ETPQAS
Sbjct: 301 ETPQAS 306



>Os11g0629200 Similar to Vacuolar sorting protein-like
          Length = 304

 Score =  539 bits (1388), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 259/306 (84%), Positives = 282/306 (92%), Gaps = 2/306 (0%)

Query: 1   MNYIVGAFKPPCDISIAFSDARSRKQIAVKNDNGRTVMVPVFQSLETISGEVSVVPVPGK 60
           MNYI+GAFK PCD+ I F+D RSRKQ+A+K DNG+T+MVP FQSLETI+GEVSV PVPGK
Sbjct: 1   MNYIIGAFKAPCDVFITFADERSRKQVAIKKDNGKTLMVPAFQSLETIAGEVSVAPVPGK 60

Query: 61  RIEHLGVKIELLGQIELYLERGKFYDFTSLVRELDIPGEIYEKKTYPFEFSTVEMPYESY 120
           R+EH GVKIELLGQIELY ERG FYDFTSLVREL++ GEIYE+KTYPFEFSTVEMPYESY
Sbjct: 61  RVEHQGVKIELLGQIELYHERGHFYDFTSLVRELEVAGEIYERKTYPFEFSTVEMPYESY 120

Query: 121 NGTNVRLRYILKVTISRNIMGMGSIVEFREFCVRNYSPAPTINNSIKMEVGIEDCLHIEF 180
           NGTNVRLRYILKVTI R      S+VE R+FCVRNY+P P+INNSIKMEVGIEDCLHIEF
Sbjct: 121 NGTNVRLRYILKVTIGRPYAT--SVVECRDFCVRNYTPLPSINNSIKMEVGIEDCLHIEF 178

Query: 181 EYSKSKYHLKDVIIGKIYFLLVRIKIKNMELEVRRRESTGAGSSAYVETETLAKFELMDG 240
           EYSKSKYHLKDVI+GKIYFLLVRIKIKNMELE+RRRESTG+G   Y+ETETLAKFELMDG
Sbjct: 179 EYSKSKYHLKDVIVGKIYFLLVRIKIKNMELEIRRRESTGSGPGTYIETETLAKFELMDG 238

Query: 241 APVRGESIPVRLFLTPYELTPSYRNINNKFSVKYYLNLVLVDEEDRRYFKQQEITMYRLL 300
           APVRGESIPVRLFLTPYELTP+YRNINNKF+VKYYLNLVLVDEEDRRYFKQQEITMYRL 
Sbjct: 239 APVRGESIPVRLFLTPYELTPTYRNINNKFNVKYYLNLVLVDEEDRRYFKQQEITMYRLQ 298

Query: 301 ETPQAS 306
           ETPQ+S
Sbjct: 299 ETPQSS 304



>Os03g0679100 Vacuolar protein sorting-associated protein 26 family protein
          Length = 58

 Score = 92.8 bits (229), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 44/57 (77%), Positives = 48/57 (84%)

Query: 38 MVPVFQSLETISGEVSVVPVPGKRIEHLGVKIELLGQIELYLERGKFYDFTSLVREL 94
          MV  FQSLETI+GEVS+ P+PGKR+EH GVKIELLGQIELY ERG FYDFTSL   L
Sbjct: 1  MVLAFQSLETIAGEVSIAPIPGKRVEHQGVKIELLGQIELYHERGHFYDFTSLGSSL 57



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.139    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,707,385
Number of extensions: 389956
Number of successful extensions: 666
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 665
Number of HSP's successfully gapped: 3
Length of query: 306
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 205
Effective length of database: 11,762,187
Effective search space: 2411248335
Effective search space used: 2411248335
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)