BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os12g0421000 Os12g0421000|AK071491
(239 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os12g0421000 Similar to Barley stem rust resistance protein 150 7e-37
Os03g0126700 Similar to Barley stem rust resistance protein 137 5e-33
Os10g0532300 Heavy metal transport/detoxification protein d... 111 6e-25
>Os12g0421000 Similar to Barley stem rust resistance protein
Length = 239
Score = 150 bits (380), Expect = 7e-37, Method: Compositional matrix adjust.
Identities = 75/75 (100%), Positives = 75/75 (100%)
Query: 1 MSKKIVVKLDLHDNKDKQKAMKAVSSVIGIDAISMDMASRKMTVIGTVDPVDVVSKLRKA 60
MSKKIVVKLDLHDNKDKQKAMKAVSSVIGIDAISMDMASRKMTVIGTVDPVDVVSKLRKA
Sbjct: 1 MSKKIVVKLDLHDNKDKQKAMKAVSSVIGIDAISMDMASRKMTVIGTVDPVDVVSKLRKA 60
Query: 61 SWAAYIESVGPAKEP 75
SWAAYIESVGPAKEP
Sbjct: 61 SWAAYIESVGPAKEP 75
>Os03g0126700 Similar to Barley stem rust resistance protein
Length = 195
Score = 137 bits (346), Expect = 5e-33, Method: Compositional matrix adjust.
Identities = 68/75 (90%), Positives = 72/75 (96%)
Query: 1 MSKKIVVKLDLHDNKDKQKAMKAVSSVIGIDAISMDMASRKMTVIGTVDPVDVVSKLRKA 60
MSKKIVVKLDLHDNKDKQKAMK VS++ GIDAISMDMASRKMTVIGTVDPV+VVSKLRKA
Sbjct: 1 MSKKIVVKLDLHDNKDKQKAMKVVSTLAGIDAISMDMASRKMTVIGTVDPVNVVSKLRKA 60
Query: 61 SWAAYIESVGPAKEP 75
SW AYIES+GPAKEP
Sbjct: 61 SWPAYIESLGPAKEP 75
>Os10g0532300 Heavy metal transport/detoxification protein domain containing
protein
Length = 179
Score = 111 bits (277), Expect = 6e-25, Method: Compositional matrix adjust.
Identities = 58/72 (80%), Positives = 66/72 (91%), Gaps = 1/72 (1%)
Query: 1 MSKKIVVKLDLHDNKDKQKAMKAVSSVIGIDAISMDMASRKMTVIGTVDPVDVVSKLRKA 60
MSKKIVVKL++HD +KQKAMKAVS++IGID +SMDMAS+KMTVIG VDPV+VVSKLRK
Sbjct: 1 MSKKIVVKLNVHDKAEKQKAMKAVSALIGIDELSMDMASQKMTVIGMVDPVNVVSKLRK- 59
Query: 61 SWAAYIESVGPA 72
SWAA IESVGPA
Sbjct: 60 SWAATIESVGPA 71
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.316 0.127 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,861,973
Number of extensions: 60988
Number of successful extensions: 208
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 207
Number of HSP's successfully gapped: 5
Length of query: 239
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 141
Effective length of database: 11,918,829
Effective search space: 1680554889
Effective search space used: 1680554889
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 154 (63.9 bits)