BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0210300 Os12g0210300|AK065921
         (212 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0210300  Similar to Glutathione S-transferase GST 18 (E...   404   e-113
AK073415                                                          404   e-113
Os12g0210200  Similar to Glutathione S-transferase GST 18 (E...   315   1e-86
Os02g0564000  Similar to Glutathione S-transferase                238   2e-63
Os11g0245100  Glutathione S-transferase, N-terminal domain c...    70   9e-13

>Os12g0210300 Similar to Glutathione S-transferase GST 18 (EC 2.5.1.18)
          Length = 212

 Score =  404 bits (1037), Expect = e-113,   Method: Compositional matrix adjust.
 Identities = 199/212 (93%), Positives = 199/212 (93%)

Query: 1   MASSKPILYGAWISSCSHRIRIVLNLKGVDYEYKSVNPRTDPDYEKINPIKYIPALVDGD 60
           MASSKPILYGAWISSCSHRIRIVLNLKGVDYEYKSVNPRTDPDYEKINPIKYIPALVDGD
Sbjct: 1   MASSKPILYGAWISSCSHRIRIVLNLKGVDYEYKSVNPRTDPDYEKINPIKYIPALVDGD 60

Query: 61  LVVSDSLAIALYLEDKYPQHAXXXXXXXXXXXXXQIANIVCSSIQPLQGYAVIGLHEGKL 120
           LVVSDSLAIALYLEDKYPQHA             QIANIVCSSIQPLQGYAVIGLHEGKL
Sbjct: 61  LVVSDSLAIALYLEDKYPQHALLPKDLKKKALNLQIANIVCSSIQPLQGYAVIGLHEGKL 120

Query: 121 SPDESLQIVQHYIDKGFKAIEKLLEGSNFKYATGDEVQLGDVFLAPQIHAGINRFQIDIT 180
           SPDESLQIVQHYIDKGFKAIEKLLEGSNFKYATGDEVQLGDVFLAPQIHAGINRFQIDIT
Sbjct: 121 SPDESLQIVQHYIDKGFKAIEKLLEGSNFKYATGDEVQLGDVFLAPQIHAGINRFQIDIT 180

Query: 181 KYPNLARLHDTYMEIPAFQAALPKNQPDAPSC 212
           KYPNLARLHDTYMEIPAFQAALPKNQPDAPSC
Sbjct: 181 KYPNLARLHDTYMEIPAFQAALPKNQPDAPSC 212



>AK073415 
          Length = 212

 Score =  404 bits (1037), Expect = e-113,   Method: Compositional matrix adjust.
 Identities = 199/212 (93%), Positives = 199/212 (93%)

Query: 1   MASSKPILYGAWISSCSHRIRIVLNLKGVDYEYKSVNPRTDPDYEKINPIKYIPALVDGD 60
           MASSKPILYGAWISSCSHRIRIVLNLKGVDYEYKSVNPRTDPDYEKINPIKYIPALVDGD
Sbjct: 1   MASSKPILYGAWISSCSHRIRIVLNLKGVDYEYKSVNPRTDPDYEKINPIKYIPALVDGD 60

Query: 61  LVVSDSLAIALYLEDKYPQHAXXXXXXXXXXXXXQIANIVCSSIQPLQGYAVIGLHEGKL 120
           LVVSDSLAIALYLEDKYPQHA             QIANIVCSSIQPLQGYAVIGLHEGKL
Sbjct: 61  LVVSDSLAIALYLEDKYPQHALLPKDLKKKALNLQIANIVCSSIQPLQGYAVIGLHEGKL 120

Query: 121 SPDESLQIVQHYIDKGFKAIEKLLEGSNFKYATGDEVQLGDVFLAPQIHAGINRFQIDIT 180
           SPDESLQIVQHYIDKGFKAIEKLLEGSNFKYATGDEVQLGDVFLAPQIHAGINRFQIDIT
Sbjct: 121 SPDESLQIVQHYIDKGFKAIEKLLEGSNFKYATGDEVQLGDVFLAPQIHAGINRFQIDIT 180

Query: 181 KYPNLARLHDTYMEIPAFQAALPKNQPDAPSC 212
           KYPNLARLHDTYMEIPAFQAALPKNQPDAPSC
Sbjct: 181 KYPNLARLHDTYMEIPAFQAALPKNQPDAPSC 212



>Os12g0210200 Similar to Glutathione S-transferase GST 18 (EC 2.5.1.18)
          Length = 214

 Score =  315 bits (807), Expect = 1e-86,   Method: Compositional matrix adjust.
 Identities = 151/208 (72%), Positives = 171/208 (82%), Gaps = 1/208 (0%)

Query: 4   SKPILYGAWISSCSHRIRIVLNLKGVDYEYKSVNPRTDPDYEKINPIKYIPALVDGDLVV 63
           +KP+LY  W+SSCS+R+RI LNLKG+DYEY++V  R DPDY KINPIKY+PALVDGD  +
Sbjct: 7   TKPVLYSEWMSSCSYRVRIALNLKGIDYEYRAVT-RGDPDYGKINPIKYVPALVDGDFTI 65

Query: 64  SDSLAIALYLEDKYPQHAXXXXXXXXXXXXXQIANIVCSSIQPLQGYAVIGLHEGKLSPD 123
           SDSLAI LYLEDKYPQH              QIANIVCSSIQPLQ YAVIGL +GK+S +
Sbjct: 66  SDSLAIILYLEDKYPQHPLLPQDLKKKALNMQIANIVCSSIQPLQCYAVIGLADGKMSAN 125

Query: 124 ESLQIVQHYIDKGFKAIEKLLEGSNFKYATGDEVQLGDVFLAPQIHAGINRFQIDITKYP 183
           ESLQIVQHY DKGF+AIEKLLEG   KYATGDEVQL DVFLAPQIHAGI RFQID++KYP
Sbjct: 126 ESLQIVQHYTDKGFRAIEKLLEGCRSKYATGDEVQLADVFLAPQIHAGITRFQIDMSKYP 185

Query: 184 NLARLHDTYMEIPAFQAALPKNQPDAPS 211
            LAR +  YME+PAFQAA+P+NQPDAPS
Sbjct: 186 ILARFYKAYMELPAFQAAVPENQPDAPS 213



>Os02g0564000 Similar to Glutathione S-transferase
          Length = 237

 Score =  238 bits (608), Expect = 2e-63,   Method: Compositional matrix adjust.
 Identities = 122/220 (55%), Positives = 149/220 (67%), Gaps = 16/220 (7%)

Query: 8   LYGAWISSCSHRIRIVLNLKGVDYEYKSVN----PRTDPDYEKINPIKYIPALVDGDLVV 63
           LY  W SSCSHR+RI LNLKG++YEYK+VN      +DP++ K+NP+K++PALVDGD V+
Sbjct: 16  LYSYWRSSCSHRVRIALNLKGLEYEYKAVNLLKGEHSDPEFMKVNPMKFVPALVDGDAVI 75

Query: 64  SDSLAIALYLEDKYPQHAXXXXXXXXXXXXXQIANIVCSSIQPLQGYAVI---GLH---- 116
            DS AIALYLEDKYP+H              QIA+IVCS IQPL    V+    LH    
Sbjct: 76  GDSYAIALYLEDKYPEHPLLPQDLKMKALNLQIASIVCSGIQPLHNLTVLVRTDLHSISY 135

Query: 117 -----EGKLSPDESLQIVQHYIDKGFKAIEKLLEGSNFKYATGDEVQLGDVFLAPQIHAG 171
                E K+   ES+   Q  ID+GF A E L++G   KYATGDEV+L DVFLAPQI+A 
Sbjct: 136 CHRFIEKKVGTGESIPWTQQQIDRGFAAAENLVKGCAGKYATGDEVRLADVFLAPQIYAA 195

Query: 172 INRFQIDITKYPNLARLHDTYMEIPAFQAALPKNQPDAPS 211
           + RFQI++  YP LARLH+ YM+ PAFQAALP  QPDAPS
Sbjct: 196 VTRFQINMLNYPTLARLHEEYMKHPAFQAALPDRQPDAPS 235



>Os11g0245100 Glutathione S-transferase, N-terminal domain containing protein
          Length = 104

 Score = 70.5 bits (171), Expect = 9e-13,   Method: Compositional matrix adjust.
 Identities = 30/53 (56%), Positives = 42/53 (79%), Gaps = 4/53 (7%)

Query: 8  LYGAWISSCSHRIRIVLNLKGVDYEYKSV----NPRTDPDYEKINPIKYIPAL 56
          LY  W SSCS+R+RI L+LKG+DYEYK +    N ++ P++EK+NP+KY+PAL
Sbjct: 28 LYSFWRSSCSYRVRIALSLKGLDYEYKPINLLANEQSHPEFEKLNPMKYVPAL 80



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.138    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,919,139
Number of extensions: 264864
Number of successful extensions: 555
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 553
Number of HSP's successfully gapped: 5
Length of query: 212
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 115
Effective length of database: 11,971,043
Effective search space: 1376669945
Effective search space used: 1376669945
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 154 (63.9 bits)