BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0129300 Os12g0129300|AK062739
         (89 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0129300  Similar to Arabinoxylan arabinofuranohydrolase...   133   2e-32
Os11g0132600  Similar to Arabinoxylan arabinofuranohydrolase...    93   5e-20
Os11g0131900  Similar to Arabinoxylan arabinofuranohydrolase...    82   1e-16
Os12g0128700  Similar to Arabinoxylan arabinofuranohydrolase...    80   3e-16

>Os12g0129300 Similar to Arabinoxylan arabinofuranohydrolase isoenzyme AXAH-II
          Length = 89

 Score =  133 bits (335), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 67/89 (75%), Positives = 67/89 (75%)

Query: 1  MGSKDAXXXXXXXXXXXXXXXXXXXXXXDLPQTALVEVDASWEVSRKIPDTLFGLFFEEI 60
          MGSKDA                      DLPQTALVEVDASWEVSRKIPDTLFGLFFEEI
Sbjct: 1  MGSKDAFFCTFCSLLLFCFSSKCLSSELDLPQTALVEVDASWEVSRKIPDTLFGLFFEEI 60

Query: 61 NHAGAGGIWAELVSNRSNSQFDKHSSWKL 89
          NHAGAGGIWAELVSNRSNSQFDKHSSWKL
Sbjct: 61 NHAGAGGIWAELVSNRSNSQFDKHSSWKL 89



>Os11g0132600 Similar to Arabinoxylan arabinofuranohydrolase isoenzyme AXAH-II
          Length = 657

 Score = 92.8 bits (229), Expect = 5e-20,   Method: Compositional matrix adjust.
 Identities = 42/48 (87%), Positives = 46/48 (95%)

Query: 29 DLPQTALVEVDASWEVSRKIPDTLFGLFFEEINHAGAGGIWAELVSNR 76
          D+ QTA++EVDASW +SRKIPDTLFGLFFEEINHAGAGGIWAELVSNR
Sbjct: 29 DMAQTAVLEVDASWNLSRKIPDTLFGLFFEEINHAGAGGIWAELVSNR 76



>Os11g0131900 Similar to Arabinoxylan arabinofuranohydrolase isoenzyme AXAH-II
          Length = 658

 Score = 81.6 bits (200), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 36/45 (80%), Positives = 44/45 (97%)

Query: 32 QTALVEVDASWEVSRKIPDTLFGLFFEEINHAGAGGIWAELVSNR 76
          QTA+++VDAS +++R+IPDTLFG+FFEEINHAGAGGIWAELVSNR
Sbjct: 35 QTAVLKVDASPQLARQIPDTLFGIFFEEINHAGAGGIWAELVSNR 79



>Os12g0128700 Similar to Arabinoxylan arabinofuranohydrolase isoenzyme AXAH-II
          Length = 660

 Score = 80.5 bits (197), Expect = 3e-16,   Method: Compositional matrix adjust.
 Identities = 36/45 (80%), Positives = 43/45 (95%)

Query: 32 QTALVEVDASWEVSRKIPDTLFGLFFEEINHAGAGGIWAELVSNR 76
          QTA+++VDAS + +R+IPDTLFG+FFEEINHAGAGGIWAELVSNR
Sbjct: 37 QTAVLKVDASLQHARQIPDTLFGIFFEEINHAGAGGIWAELVSNR 81



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.130    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,529,928
Number of extensions: 72751
Number of successful extensions: 123
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 123
Number of HSP's successfully gapped: 4
Length of query: 89
Length of database: 17,035,801
Length adjustment: 59
Effective length of query: 30
Effective length of database: 13,955,175
Effective search space: 418655250
Effective search space used: 418655250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 149 (62.0 bits)