BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0125300 Os12g0125300|AK102502
         (622 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0125300  Mad3/BUB1 homology region 1 domain containing ...  1285   0.0  
Os11g0128700  Mad3/BUB1 homology region 1 domain containing ...   109   5e-24
Os02g0193600  Mad3/BUB1 homology region 1 domain containing ...    94   3e-19

>Os12g0125300 Mad3/BUB1 homology region 1 domain containing protein
          Length = 622

 Score = 1285 bits (3326), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 617/622 (99%), Positives = 617/622 (99%)

Query: 1   MAAATEQHCXXXXXDKEKDLLSAVVGDIRSYSGSDPLRPWLRGMRKMEAALPPATLRAKL 60
           MAAATEQHC     DKEKDLLSAVVGDIRSYSGSDPLRPWLRGMRKMEAALPPATLRAKL
Sbjct: 1   MAAATEQHCGGGGGDKEKDLLSAVVGDIRSYSGSDPLRPWLRGMRKMEAALPPATLRAKL 60

Query: 61  PRFLQKCAQEFQDDARYRDDSRYLRVWIQLMDYVKDAKPLLKKMEKNRIGLKRSAFYMAY 120
           PRFLQKCAQEFQDDARYRDDSRYLRVWIQLMDYVKDAKPLLKKMEKNRIGLKRSAFYMAY
Sbjct: 61  PRFLQKCAQEFQDDARYRDDSRYLRVWIQLMDYVKDAKPLLKKMEKNRIGLKRSAFYMAY 120

Query: 121 AVYYEKHKRFEDAENMYRLGTQNLAEPVGELQKAHEQFIRRVELYKRRKSRVQQERMPNK 180
           AVYYEKHKRFEDAENMYRLGTQNLAEPVGELQKAHEQFIRRVELYKRRKSRVQQERMPNK
Sbjct: 121 AVYYEKHKRFEDAENMYRLGTQNLAEPVGELQKAHEQFIRRVELYKRRKSRVQQERMPNK 180

Query: 181 VQSIAISKNEVEGQSRSCTKPKSNPVQRSGSGSNPHLGFPHPLGRPLSRGTSGETMSLSR 240
           VQSIAISKNEVEGQSRSCTKPKSNPVQRSGSGSNPHLGFPHPLGRPLSRGTSGETMSLSR
Sbjct: 181 VQSIAISKNEVEGQSRSCTKPKSNPVQRSGSGSNPHLGFPHPLGRPLSRGTSGETMSLSR 240

Query: 241 HNSDDTVVVRFVGSALVGKSETEDACHHGLVEPTINTKEAMDAISSMFLEPLEPETKLKR 300
           HNSDDTVVVRFVGSALVGKSETEDACHHGLVEPTINTKEAMDAISSMFLEPLEPETKLKR
Sbjct: 241 HNSDDTVVVRFVGSALVGKSETEDACHHGLVEPTINTKEAMDAISSMFLEPLEPETKLKR 300

Query: 301 RSNRDKPSFNQEASAFEIFVDEDEPNKSGPSKLQDKNMKQDNPKLSQQASAFEIFVDEDD 360
           RSNRDKPSFNQEASAFEIFVDEDEPNKSGPSKLQDKNMKQDNPKLSQQASAFEIFVDEDD
Sbjct: 301 RSNRDKPSFNQEASAFEIFVDEDEPNKSGPSKLQDKNMKQDNPKLSQQASAFEIFVDEDD 360

Query: 361 PYCNNQNMVQHRHFNKENTQVNQNASGFEIFVDENEAHGNGRNAMSHKSSGCPPKPSRDS 420
           PYCNNQNMVQHRHFNKENTQVNQNASGFEIFVDENEAHGNGRNAMSHKSSGCPPKPSRDS
Sbjct: 361 PYCNNQNMVQHRHFNKENTQVNQNASGFEIFVDENEAHGNGRNAMSHKSSGCPPKPSRDS 420

Query: 421 KQQANFDFQKPFVGGFAILPDDEDEQLEKNDNGVKINSGTVQLTDDKDTSLCSRQTDSKI 480
           KQQANFDFQKPFVGGFAILPDDEDEQLEKNDNGVKINSGTVQLTDDKDTSLCSRQTDSKI
Sbjct: 421 KQQANFDFQKPFVGGFAILPDDEDEQLEKNDNGVKINSGTVQLTDDKDTSLCSRQTDSKI 480

Query: 481 RCDDLRPAISGLREDTVFHRFVGSAVVGEPKVENACHHGLVEPTVNLKEAMDDINNMFGI 540
           RCDDLRPAISGLREDTVFHRFVGSAVVGEPKVENACHHGLVEPTVNLKEAMDDINNMFGI
Sbjct: 481 RCDDLRPAISGLREDTVFHRFVGSAVVGEPKVENACHHGLVEPTVNLKEAMDDINNMFGI 540

Query: 541 PLNFKGDKPKNKKTTALSERKAALLSGFSILADDEPGENPAAQVKPSNASKFECQSGLFE 600
           PLNFKGDKPKNKKTTALSERKAALLSGFSILADDEPGENPAAQVKPSNASKFECQSGLFE
Sbjct: 541 PLNFKGDKPKNKKTTALSERKAALLSGFSILADDEPGENPAAQVKPSNASKFECQSGLFE 600

Query: 601 PTITTRDVMAEINDMFGMPLDF 622
           PTITTRDVMAEINDMFGMPLDF
Sbjct: 601 PTITTRDVMAEINDMFGMPLDF 622



>Os11g0128700 Mad3/BUB1 homology region 1 domain containing protein
          Length = 53

 Score =  109 bits (273), Expect = 5e-24,   Method: Compositional matrix adjust.
 Identities = 51/52 (98%), Positives = 52/52 (100%)

Query: 91  MDYVKDAKPLLKKMEKNRIGLKRSAFYMAYAVYYEKHKRFEDAENMYRLGTQ 142
           MDYVKDAKPLLKKMEKNRIGLKRSAFYMAYA+YYEKHKRFEDAENMYRLGTQ
Sbjct: 1   MDYVKDAKPLLKKMEKNRIGLKRSAFYMAYALYYEKHKRFEDAENMYRLGTQ 52



>Os02g0193600 Mad3/BUB1 homology region 1 domain containing protein
          Length = 398

 Score = 94.0 bits (232), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 40/138 (28%), Positives = 78/138 (56%)

Query: 24  VVGDIRSYSGSDPLRPWLRGMRKMEAALPPATLRAKLPRFLQKCAQEFQDDARYRDDSRY 83
           ++  I  YSG DPL+PW+  ++ ++ + P     + L    ++C + F  D RY++D RY
Sbjct: 84  MIEAIDEYSGEDPLQPWIDCIKWVQESFPTGGDCSGLVVIYEQCVRAFWHDDRYKNDLRY 143

Query: 84  LRVWIQLMDYVKDAKPLLKKMEKNRIGLKRSAFYMAYAVYYEKHKRFEDAENMYRLGTQN 143
           L+VW++      D++ + + +E N+IG   + +Y++YA   E   + + A  ++ LG   
Sbjct: 144 LKVWLEYAGNCADSEVIFRFLEANQIGQSHTNYYLSYASVMESKNKLKKANEIFNLGIAR 203

Query: 144 LAEPVGELQKAHEQFIRR 161
            A+PV +L+  +  F++R
Sbjct: 204 KAKPVEKLETTYRAFLQR 221



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.131    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 21,824,619
Number of extensions: 968282
Number of successful extensions: 1988
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1978
Number of HSP's successfully gapped: 3
Length of query: 622
Length of database: 17,035,801
Length adjustment: 107
Effective length of query: 515
Effective length of database: 11,448,903
Effective search space: 5896185045
Effective search space used: 5896185045
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 159 (65.9 bits)