BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0116700 Os12g0116700|J065143F14
         (172 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0116700  Similar to WRKY transcription factor 64             287   2e-78
Os11g0117500  DNA-binding WRKY domain containing protein          259   5e-70
Os05g0233100                                                      118   2e-27
Os11g0117400  DNA-binding WRKY domain containing protein           68   4e-12

>Os12g0116700 Similar to WRKY transcription factor 64
          Length = 172

 Score =  287 bits (735), Expect = 2e-78,   Method: Compositional matrix adjust.
 Identities = 145/172 (84%), Positives = 145/172 (84%)

Query: 1   MKNSSNKRPLVADQWHPSSVCCDHRAALREIARGQSLVTQLRAIVLPALHSDERGDLAAQ 60
           MKNSSNKRPLVADQWHPSSVCCDHRAALREIARGQSLVTQLRAIVLPALHSDERGDLAAQ
Sbjct: 1   MKNSSNKRPLVADQWHPSSVCCDHRAALREIARGQSLVTQLRAIVLPALHSDERGDLAAQ 60

Query: 61  MLEGILDCSRKAISELQLQXXXXXXXXXXXXXXXKRRVRKIVXXXXXXXXXXXXKAAEDH 120
           MLEGILDCSRKAISELQLQ               KRRVRKIV            KAAEDH
Sbjct: 61  MLEGILDCSRKAISELQLQLSSDSPHDDDGHLDDKRRVRKIVSSSSDDDDHSSSKAAEDH 120

Query: 121 NAKPLRQHKRRRFGDSVSLETPVPHYDGHQWRKYGQKHINNSKHPRYVIRHS 172
           NAKPLRQHKRRRFGDSVSLETPVPHYDGHQWRKYGQKHINNSKHPRYVIRHS
Sbjct: 121 NAKPLRQHKRRRFGDSVSLETPVPHYDGHQWRKYGQKHINNSKHPRYVIRHS 172



>Os11g0117500 DNA-binding WRKY domain containing protein
          Length = 320

 Score =  259 bits (663), Expect = 5e-70,   Method: Compositional matrix adjust.
 Identities = 132/170 (77%), Positives = 136/170 (80%), Gaps = 1/170 (0%)

Query: 1   MKNSSNKRPLVADQWHPSSVCCDHRAALREIARGQSLVTQLRAIVLPALHSDERGDLAAQ 60
           MKNSSNKR LVADQWHPSSVCCDHRAALREIA+GQSLVTQLRAIVLPALHSDER DLAAQ
Sbjct: 1   MKNSSNKRSLVADQWHPSSVCCDHRAALREIAKGQSLVTQLRAIVLPALHSDERCDLAAQ 60

Query: 61  MLEGILDCSRKAISELQLQXXXXXXXXXXXXXXXKRRVRKIVXXXXXXXXXXXXKAAEDH 120
           MLEGILDCSRKA+S+LQL                KRRVRKI+            KAAED 
Sbjct: 61  MLEGILDCSRKAVSQLQLLLSSPHDDDDHHHVDDKRRVRKII-SSSDDDDHCSSKAAEDD 119

Query: 121 NAKPLRQHKRRRFGDSVSLETPVPHYDGHQWRKYGQKHINNSKHPRYVIR 170
           NAKPLRQHKRRRFGDSVSLETPVPHYDGHQWRKYGQKHINNSKHPR   R
Sbjct: 120 NAKPLRQHKRRRFGDSVSLETPVPHYDGHQWRKYGQKHINNSKHPRSYYR 169



>Os05g0233100 
          Length = 321

 Score =  118 bits (296), Expect = 2e-27,   Method: Compositional matrix adjust.
 Identities = 70/154 (45%), Positives = 83/154 (53%), Gaps = 12/154 (7%)

Query: 22  CDHRAALREIARGQSLVTQLRAIVLPALHS---DERGDLAAQMLEGILDCSRKAISELQL 78
           CDHR A+ EI R QSLVTQLRA+VLPAL +   D R ++ AQ+   ILDCSRK IS L  
Sbjct: 28  CDHRPAIDEIVREQSLVTQLRAVVLPALETKADDGRAEIVAQLFGSILDCSRKVISALNS 87

Query: 79  QXXXXXXXXXXXXXXXKRRVRKIVXXXXXXXXXXXXKAAEDHNAKPLRQHKRRRFGDSVS 138
           +               +R  RK              K  +    KP    + RR+ +S S
Sbjct: 88  RYVGESPPDDDEIVDKRRAKRK---------NSEGKKGDDQVKVKPHEHKRSRRYTNSTS 138

Query: 139 LETPVPHYDGHQWRKYGQKHINNSKHPRYVIRHS 172
             T VPHYDGHQWRKYGQK+INNS H R   R S
Sbjct: 139 QITAVPHYDGHQWRKYGQKNINNSNHQRSYYRCS 172



>Os11g0117400 DNA-binding WRKY domain containing protein
          Length = 263

 Score = 67.8 bits (164), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 47/141 (33%), Positives = 66/141 (46%), Gaps = 14/141 (9%)

Query: 31  IARGQSLVTQLRAIVLPALHSD-ERGDLAAQMLEGILDCSRKAISELQLQXXXXXXXXXX 89
           I   ++L+ +LR +V+P L SD E+  LA Q+L  IL CS KAIS L+L           
Sbjct: 2   IEHQKALMVELRGMVMPLLPSDNEQAKLALQLLGDILSCSDKAISMLEL----------- 50

Query: 90  XXXXXKRRVRKIVXXXXXXXXXXXXKAAEDHNAKPLRQHKRRRFGDSVSLETPVPHYDGH 149
                 +++  +V                +  AK     +R+    + S     PH DGH
Sbjct: 51  --GGDTKKLTNLVGGKRKGDKHSMDNHNLEEEAKESVSKRRKNAEHTGSTVAQAPHNDGH 108

Query: 150 QWRKYGQKHINNSKHPRYVIR 170
           QWRKYGQK I+ +KH R   R
Sbjct: 109 QWRKYGQKWISRAKHSRSYYR 129



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.132    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,056,047
Number of extensions: 167450
Number of successful extensions: 474
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 469
Number of HSP's successfully gapped: 4
Length of query: 172
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 78
Effective length of database: 12,127,685
Effective search space: 945959430
Effective search space used: 945959430
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 152 (63.2 bits)