BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os11g0621500 Os11g0621500|AK068037
(117 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os11g0621500 Similar to EARLY FLOWERING 4 (Fragment) 236 2e-63
Os03g0410300 Protein of unknown function DUF1313 family pro... 127 3e-30
Os08g0366200 Protein of unknown function DUF1313 family pro... 93 4e-20
Os08g0366300 70 2e-13
>Os11g0621500 Similar to EARLY FLOWERING 4 (Fragment)
Length = 117
Score = 236 bits (603), Expect = 2e-63, Method: Compositional matrix adjust.
Identities = 117/117 (100%), Positives = 117/117 (100%)
Query: 1 MEGDSFSGMANGGQVDNKLIQTFHKSFVQVQSILDQNRMLINEINQNHESRAPDNLTRNV 60
MEGDSFSGMANGGQVDNKLIQTFHKSFVQVQSILDQNRMLINEINQNHESRAPDNLTRNV
Sbjct: 1 MEGDSFSGMANGGQVDNKLIQTFHKSFVQVQSILDQNRMLINEINQNHESRAPDNLTRNV 60
Query: 61 GLIRELNNNIRRVVGLYADLSASFARTMDASSEGDSSGTLRSSDGAGRSGQKRVRPG 117
GLIRELNNNIRRVVGLYADLSASFARTMDASSEGDSSGTLRSSDGAGRSGQKRVRPG
Sbjct: 61 GLIRELNNNIRRVVGLYADLSASFARTMDASSEGDSSGTLRSSDGAGRSGQKRVRPG 117
>Os03g0410300 Protein of unknown function DUF1313 family protein
Length = 114
Score = 127 bits (318), Expect = 3e-30, Method: Compositional matrix adjust.
Identities = 70/117 (59%), Positives = 83/117 (70%), Gaps = 10/117 (8%)
Query: 1 MEGDSFSGMANGGQVDNKLIQTFHKSFVQVQSILDQNRMLINEINQNHESRAPDNLTRNV 60
M+GD+ S A D K++ F SFVQVQS+LDQNR+LINEINQNHES+ P +L+RNV
Sbjct: 1 MDGDTLSAAA---AEDGKVLHAFQTSFVQVQSLLDQNRVLINEINQNHESKVPGDLSRNV 57
Query: 61 GLIRELNNNIRRVVGLYADLSASFARTM--DASSEGDSSGTLRSSDGAGRSGQKRVR 115
GLIRELNNNIRRVV LYADLS+ FA + A+SEG S GT +G KRVR
Sbjct: 58 GLIRELNNNIRRVVDLYADLSSLFAASSPGPAASEGASVGT-----AVRHAGHKRVR 109
>Os08g0366200 Protein of unknown function DUF1313 family protein
Length = 144
Score = 92.8 bits (229), Expect = 4e-20, Method: Compositional matrix adjust.
Identities = 44/71 (61%), Positives = 57/71 (80%)
Query: 18 KLIQTFHKSFVQVQSILDQNRMLINEINQNHESRAPDNLTRNVGLIRELNNNIRRVVGLY 77
K++Q ++F +VQ IL+QNR+LI EI+QNHE+R D LTRNV LIRELN NI RVV LY
Sbjct: 39 KVVQVLQRNFGEVQGILEQNRVLIQEISQNHEARDADGLTRNVALIRELNTNIARVVDLY 98
Query: 78 ADLSASFARTM 88
A+LS SF+R++
Sbjct: 99 ANLSGSFSRSV 109
>Os08g0366300
Length = 176
Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 36/65 (55%), Positives = 47/65 (72%)
Query: 19 LIQTFHKSFVQVQSILDQNRMLINEINQNHESRAPDNLTRNVGLIRELNNNIRRVVGLYA 78
++Q S +V+ IL+QN LI EI+QNH++R D LTRNV LIR+LN NI RVV LYA
Sbjct: 68 VVQDPPLSLGEVRGILEQNHTLIQEISQNHKARDADRLTRNVALIRDLNTNIARVVDLYA 127
Query: 79 DLSAS 83
+L+ S
Sbjct: 128 NLTGS 132
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.313 0.130 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,667,020
Number of extensions: 122373
Number of successful extensions: 344
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 344
Number of HSP's successfully gapped: 4
Length of query: 117
Length of database: 17,035,801
Length adjustment: 84
Effective length of query: 33
Effective length of database: 12,649,825
Effective search space: 417444225
Effective search space used: 417444225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)