BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0600500 Os11g0600500|Os11g0600500
         (191 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0600500  EF-Hand type domain containing protein              397   e-111
Os09g0412300  Calcium-binding EF-hand domain containing protein    71   6e-13
Os03g0812400  EF-Hand type domain containing protein               70   1e-12
Os11g0143800                                                       70   1e-12
Os07g0223900                                                       66   2e-11

>Os11g0600500 EF-Hand type domain containing protein
          Length = 191

 Score =  397 bits (1019), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 191/191 (100%), Positives = 191/191 (100%)

Query: 1   MEKSPAMHFLLELESQEPVCLLILFTMFTWFISKVQMILPSSCQHCNYAVTKVTSTPVLA 60
           MEKSPAMHFLLELESQEPVCLLILFTMFTWFISKVQMILPSSCQHCNYAVTKVTSTPVLA
Sbjct: 1   MEKSPAMHFLLELESQEPVCLLILFTMFTWFISKVQMILPSSCQHCNYAVTKVTSTPVLA 60

Query: 61  DRKISKNLSKHKDDGIEMTHEDVESVMTKMGPDFDHGKTMVYKEIGSNCMSELFDDDEPS 120
           DRKISKNLSKHKDDGIEMTHEDVESVMTKMGPDFDHGKTMVYKEIGSNCMSELFDDDEPS
Sbjct: 61  DRKISKNLSKHKDDGIEMTHEDVESVMTKMGPDFDHGKTMVYKEIGSNCMSELFDDDEPS 120

Query: 121 LDEVKQAFLVFDEDNDGYIDALDLYRVLRNLGLREGVGVDECEQMIAKYDMNRDRRIDMV 180
           LDEVKQAFLVFDEDNDGYIDALDLYRVLRNLGLREGVGVDECEQMIAKYDMNRDRRIDMV
Sbjct: 121 LDEVKQAFLVFDEDNDGYIDALDLYRVLRNLGLREGVGVDECEQMIAKYDMNRDRRIDMV 180

Query: 181 EFIRVLEASFF 191
           EFIRVLEASFF
Sbjct: 181 EFIRVLEASFF 191



>Os09g0412300 Calcium-binding EF-hand domain containing protein
          Length = 75

 Score = 70.9 bits (172), Expect = 6e-13,   Method: Compositional matrix adjust.
 Identities = 32/73 (43%), Positives = 47/73 (64%)

Query: 117 DEPSLDEVKQAFLVFDEDNDGYIDALDLYRVLRNLGLREGVGVDECEQMIAKYDMNRDRR 176
           +E +L E   AF VFD + DG+IDA +L  VL +LG   G G  EC++MI  YD ++D R
Sbjct: 1   EEATLGEAAAAFRVFDRNGDGFIDAGELGSVLASLGFAAGAGHAECQRMIDAYDADKDGR 60

Query: 177 IDMVEFIRVLEAS 189
           +D  EF++ +E +
Sbjct: 61  VDFREFLKFMETA 73



>Os03g0812400 EF-Hand type domain containing protein
          Length = 213

 Score = 69.7 bits (169), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 45/148 (30%), Positives = 70/148 (47%), Gaps = 9/148 (6%)

Query: 45  HCNYAVTKVTSTPVLADRKISKNLSKHKDDGIEMTHEDVESVMTKMGPDFDHGKTMVYKE 104
            C  AV  V   P L  R+  +  ++    G  ++  DV +V+  +G            E
Sbjct: 67  RCAGAVAVVEPPPPL--RRECELCARRGGAG--LSRHDVAAVVASLGMVAAGEDDDDDDE 122

Query: 105 IGSNC-----MSELFDDDEPSLDEVKQAFLVFDEDNDGYIDALDLYRVLRNLGLREGVGV 159
               C     + E+ +       E+++AF VFD D DGY+ A +L+ V+R LG+ EG   
Sbjct: 123 ACGVCEAVAAVEEMAEGKVAGEGELREAFYVFDRDEDGYVSAAELWNVMRRLGIEEGARY 182

Query: 160 DECEQMIAKYDMNRDRRIDMVEFIRVLE 187
            +C +MIA YD + D RI   EF  ++E
Sbjct: 183 GDCVRMIAAYDGDGDGRISFQEFRAMME 210



>Os11g0143800 
          Length = 146

 Score = 69.7 bits (169), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 40/106 (37%), Positives = 60/106 (56%), Gaps = 5/106 (4%)

Query: 77  EMTHEDVESVMTKMGPDFDHGKTMVYKEIGSNCMSELFDDDEPSLDEVKQAFLVFDEDND 136
           E+TH DV  VM  +G  F+     V  E        L ++ + S +E+++AF VFD D D
Sbjct: 38  ELTHGDVAVVMAALGLSFNAEGNEVEDEA-----LVLLEEKQASWEELEEAFSVFDGDGD 92

Query: 137 GYIDALDLYRVLRNLGLREGVGVDECEQMIAKYDMNRDRRIDMVEF 182
           G+I  L+L  V+R LGL+   G +ECE+M+  +D + D  I+  EF
Sbjct: 93  GFISPLELQNVMRRLGLQHDAGHEECERMLKVFDRDGDGMINFDEF 138



>Os07g0223900 
          Length = 192

 Score = 65.9 bits (159), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 34/76 (44%), Positives = 49/76 (64%)

Query: 112 ELFDDDEPSLDEVKQAFLVFDEDNDGYIDALDLYRVLRNLGLREGVGVDECEQMIAKYDM 171
           EL D    S  E+K AF VFD + DG+I A +L+ V+R LG +EG   ++C +MI  +D 
Sbjct: 109 ELLDRKMASEGELKDAFYVFDRNEDGFICASELWSVMRRLGFKEGQRYEDCMRMIHTFDE 168

Query: 172 NRDRRIDMVEFIRVLE 187
           +RD RI  +EF R++E
Sbjct: 169 DRDGRISYLEFRRMME 184



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.137    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,127,321
Number of extensions: 250629
Number of successful extensions: 910
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 927
Number of HSP's successfully gapped: 6
Length of query: 191
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 96
Effective length of database: 12,075,471
Effective search space: 1159245216
Effective search space used: 1159245216
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 153 (63.5 bits)