BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0592200 Os11g0592200|AK121127
         (146 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0592200  Similar to Chitin-binding allergen Bra r 2 (Fr...   259   4e-70
Os11g0592000  Similar to Barwin                                   206   6e-54
Os11g0592100  Similar to Barwin                                   199   4e-52
Os11g0591800  Barwin-related endoglucanase domain containing...   189   8e-49
Os11g0591700  Barwin-related endoglucanase domain containing...   136   5e-33

>Os11g0592200 Similar to Chitin-binding allergen Bra r 2 (Fragments)
          Length = 146

 Score =  259 bits (663), Expect = 4e-70,   Method: Compositional matrix adjust.
 Identities = 129/146 (88%), Positives = 129/146 (88%)

Query: 1   MAGITGSRXXXXXXXXXXXXXXXXXQEASNVRATYHYYNPQQNNWDLNKVSAYCATWDAN 60
           MAGITGSR                 QEASNVRATYHYYNPQQNNWDLNKVSAYCATWDAN
Sbjct: 1   MAGITGSRALMVVALLCAAAAMTAAQEASNVRATYHYYNPQQNNWDLNKVSAYCATWDAN 60

Query: 61  KPLSWRQKYGWTAFCGPAGPRGRDSCGKCIQVKNRGTGATIIARIVDQCSNGGLDLDYET 120
           KPLSWRQKYGWTAFCGPAGPRGRDSCGKCIQVKNRGTGATIIARIVDQCSNGGLDLDYET
Sbjct: 61  KPLSWRQKYGWTAFCGPAGPRGRDSCGKCIQVKNRGTGATIIARIVDQCSNGGLDLDYET 120

Query: 121 IFKKIDTDGRGYQMGHLQVDYKFVNC 146
           IFKKIDTDGRGYQMGHLQVDYKFVNC
Sbjct: 121 IFKKIDTDGRGYQMGHLQVDYKFVNC 146



>Os11g0592000 Similar to Barwin
          Length = 150

 Score =  206 bits (523), Expect = 6e-54,   Method: Compositional matrix adjust.
 Identities = 96/123 (78%), Positives = 106/123 (86%), Gaps = 2/123 (1%)

Query: 26  QEASNVRATYHYYNPQQNNWDLNK--VSAYCATWDANKPLSWRQKYGWTAFCGPAGPRGR 83
           QEASNVRATYHYY P QNNWDL    VSAYCATWDANKPLSWRQKYGWTAFCGP GPRG+
Sbjct: 25  QEASNVRATYHYYRPAQNNWDLGAPAVSAYCATWDANKPLSWRQKYGWTAFCGPVGPRGQ 84

Query: 84  DSCGKCIQVKNRGTGATIIARIVDQCSNGGLDLDYETIFKKIDTDGRGYQMGHLQVDYKF 143
            +CGKC+ V N  TGA I ARIVDQC+NGGLDLD++T+F KIDTDG+GYQ GHL V+YKF
Sbjct: 85  AACGKCLLVTNTATGAQITARIVDQCANGGLDLDWDTVFTKIDTDGQGYQKGHLIVNYKF 144

Query: 144 VNC 146
           V+C
Sbjct: 145 VDC 147



>Os11g0592100 Similar to Barwin
          Length = 152

 Score =  199 bits (507), Expect = 4e-52,   Method: Compositional matrix adjust.
 Identities = 91/123 (73%), Positives = 104/123 (84%), Gaps = 2/123 (1%)

Query: 26  QEASNVRATYHYYNPQQNNWDLNK--VSAYCATWDANKPLSWRQKYGWTAFCGPAGPRGR 83
           QEASNVRATYHYY P +NNWDL    VSAYCATWDA+KPL WRQKYGWTAFCGP GP G+
Sbjct: 27  QEASNVRATYHYYRPAENNWDLGAPAVSAYCATWDADKPLEWRQKYGWTAFCGPVGPTGQ 86

Query: 84  DSCGKCIQVKNRGTGATIIARIVDQCSNGGLDLDYETIFKKIDTDGRGYQMGHLQVDYKF 143
           D+CGKC+ V N  TG  I ARIVDQC+NGGLDLD++T+F KID+DG+GYQ GHL VDY+F
Sbjct: 87  DACGKCLSVTNTATGDQITARIVDQCANGGLDLDWDTVFSKIDSDGQGYQNGHLIVDYQF 146

Query: 144 VNC 146
           V+C
Sbjct: 147 VDC 149



>Os11g0591800 Barwin-related endoglucanase domain containing protein
          Length = 158

 Score =  189 bits (479), Expect = 8e-49,   Method: Compositional matrix adjust.
 Identities = 82/121 (67%), Positives = 95/121 (78%)

Query: 26  QEASNVRATYHYYNPQQNNWDLNKVSAYCATWDANKPLSWRQKYGWTAFCGPAGPRGRDS 85
           Q+A  VRATYH+Y P  N WDL    A+C+TWDA KP  WR KY WTAFCGP GP GRDS
Sbjct: 27  QQAFGVRATYHFYRPAANGWDLTATGAFCSTWDAGKPFDWRSKYEWTAFCGPVGPTGRDS 86

Query: 86  CGKCIQVKNRGTGATIIARIVDQCSNGGLDLDYETIFKKIDTDGRGYQMGHLQVDYKFVN 145
           CGKC++V NR TGA   ARIVD+C+NGGLDLD++T+F KIDTDG+G+Q GHL VDY FVN
Sbjct: 87  CGKCLRVTNRVTGAQTTARIVDKCANGGLDLDWDTVFSKIDTDGQGFQRGHLTVDYSFVN 146

Query: 146 C 146
           C
Sbjct: 147 C 147



>Os11g0591700 Barwin-related endoglucanase domain containing protein
          Length = 167

 Score =  136 bits (343), Expect = 5e-33,   Method: Compositional matrix adjust.
 Identities = 73/142 (51%), Positives = 86/142 (60%), Gaps = 27/142 (19%)

Query: 28  ASNVRATYHYYNPQQNNWDLNKVSAYCATWDANKPLSWRQKYGWTAFCGPAGPRGRDSCG 87
           AS V ATY   NP   NWDL  VSAYC TWDA+ PL+WR+ YGWTAFCGPAG  G  SC 
Sbjct: 28  ASGVVATY---NPDTINWDLRAVSAYCLTWDADMPLAWRRCYGWTAFCGPAGAHGEPSCA 84

Query: 88  -----------------KCIQ------VKNRGTGATIIARIVDQCSNGGLDLDYETIFKK 124
                             C++      V N  T A+ +AR+VDQCS GGLDLD   +F++
Sbjct: 85  TRRACGEDCVGVGVEQFACMRDAARVGVTNTATAASAVARVVDQCSTGGLDLDV-AVFRQ 143

Query: 125 IDTDGRGYQMGHLQVDYKFVNC 146
           IDTDG G   GHL VDY+FV+C
Sbjct: 144 IDTDGGGMANGHLVVDYEFVDC 165



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.136    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,030,411
Number of extensions: 211282
Number of successful extensions: 428
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 424
Number of HSP's successfully gapped: 5
Length of query: 146
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 55
Effective length of database: 12,284,327
Effective search space: 675637985
Effective search space used: 675637985
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 151 (62.8 bits)