BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0588300 Os11g0588300|AF402793
         (246 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0588300  Similar to Glutathione transferase AtGST 10 (E...   505   e-143
Os11g0588500  Glutathione S-transferase, C-terminal-like dom...   172   3e-43
Os03g0135100  Glutathione S-transferase GSTF15                     67   1e-11
Os01g0933900  Similar to Glutathione transferase III(B) (EC ...    67   1e-11

>Os11g0588300 Similar to Glutathione transferase AtGST 10 (EC 2.5.1.18)
          Length = 246

 Score =  505 bits (1300), Expect = e-143,   Method: Compositional matrix adjust.
 Identities = 246/246 (100%), Positives = 246/246 (100%)

Query: 1   MQPLLKVYADRRSQPSRAIIIFCRVNRIDFEEVTVDLFKREHLSPEFKKINPMGQVPAIV 60
           MQPLLKVYADRRSQPSRAIIIFCRVNRIDFEEVTVDLFKREHLSPEFKKINPMGQVPAIV
Sbjct: 1   MQPLLKVYADRRSQPSRAIIIFCRVNRIDFEEVTVDLFKREHLSPEFKKINPMGQVPAIV 60

Query: 61  DGRFRLFESHAILRYLATVFPGVADHWYPADLFTRAKLEAILDWHHSNLRRGAATFILNT 120
           DGRFRLFESHAILRYLATVFPGVADHWYPADLFTRAKLEAILDWHHSNLRRGAATFILNT
Sbjct: 61  DGRFRLFESHAILRYLATVFPGVADHWYPADLFTRAKLEAILDWHHSNLRRGAATFILNT 120

Query: 121 VLAPSLGLPSSPQAAKEAEKVLFRSLGLIESMWLKGNAKFLLGNPQLSIADLSLVCEIMQ 180
           VLAPSLGLPSSPQAAKEAEKVLFRSLGLIESMWLKGNAKFLLGNPQLSIADLSLVCEIMQ
Sbjct: 121 VLAPSLGLPSSPQAAKEAEKVLFRSLGLIESMWLKGNAKFLLGNPQLSIADLSLVCEIMQ 180

Query: 181 LEVLGDSERDRILGPHEKIRSWVQNVKKATSPHFDEVHELIFKMKERMAAKRQSEPSKDL 240
           LEVLGDSERDRILGPHEKIRSWVQNVKKATSPHFDEVHELIFKMKERMAAKRQSEPSKDL
Sbjct: 181 LEVLGDSERDRILGPHEKIRSWVQNVKKATSPHFDEVHELIFKMKERMAAKRQSEPSKDL 240

Query: 241 KTASKL 246
           KTASKL
Sbjct: 241 KTASKL 246



>Os11g0588500 Glutathione S-transferase, C-terminal-like domain containing
           protein
          Length = 95

 Score =  172 bits (435), Expect = 3e-43,   Method: Compositional matrix adjust.
 Identities = 84/95 (88%), Positives = 86/95 (90%)

Query: 152 MWLKGNAKFLLGNPQLSIADLSLVCEIMQLEVLGDSERDRILGPHEKIRSWVQNVKKATS 211
           MWLKGNAKFLLGNPQLS+ADLSLVCEIMQLEVL DSERDRILGPHEKIR WVQNVKKATS
Sbjct: 1   MWLKGNAKFLLGNPQLSMADLSLVCEIMQLEVLSDSERDRILGPHEKIRGWVQNVKKATS 60

Query: 212 PHFDEVHELIFKMKERMAAKRQSEPSKDLKTASKL 246
           PHFDEVHELI K+K R AA   SE SKDLKTASKL
Sbjct: 61  PHFDEVHELILKVKTRTAATLGSELSKDLKTASKL 95



>Os03g0135100 Glutathione S-transferase GSTF15
          Length = 225

 Score = 67.0 bits (162), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 51/175 (29%), Positives = 83/175 (47%), Gaps = 6/175 (3%)

Query: 1   MQPLLKVYADRRSQPSRAIIIFCRVNRIDFEEVTVDLFKREHLSPEFKKI-NPMGQVPAI 59
           M   L+V+    S     ++       ++FE + +D FK+EH  PEF K+ +P GQV   
Sbjct: 1   MAAGLQVFGQPASTDVARVLTCLFEKNLEFELIRIDTFKKEHKLPEFIKLRDPTGQV-TF 59

Query: 60  VDGRFRLFESHAILRYLATVFPGVADHW-YPADLFTRAKLEAILDWHHSNLRRGAATFIL 118
             G   L +S AI RYL+T FP   +   Y      RA +E  L     +    ++  + 
Sbjct: 60  KHGDKTLVDSRAICRYLSTQFPDDGNRTIYGTGSLERASIEQWLQAEAQSFDAPSSELVF 119

Query: 119 NTVLAPSLGLPSSPQAAKEAEKVLFRSLGLIESMWLKGNAKFLLGNPQLSIADLS 173
           +   AP L +P+      E E+ L + L + + +  K   K+L G+ + ++ADLS
Sbjct: 120 HLAFAPQLNIPADEARIAENERKLQQMLNVYDEILAKN--KYLAGD-EFTLADLS 171



>Os01g0933900 Similar to Glutathione transferase III(B) (EC 2.5.1.18)
          Length = 221

 Score = 67.0 bits (162), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 53/202 (26%), Positives = 93/202 (46%), Gaps = 7/202 (3%)

Query: 5   LKVYADRRSQPSRAIIIFCRVNRIDFEEVTVDLFKREHLSPEFKKINPMGQVPAIVDGRF 64
           L+VY    S     +        +DF+ V VDL    H  P F  +NP GQ+P + DG  
Sbjct: 8   LRVYGMALSANVVRVATVLNEKGLDFDLVPVDLRTAAHKQPHFLALNPFGQIPVLQDGDE 67

Query: 65  RLFESHAILRYLATVFPGVADHWYPADLFTRAKLEAILDWHHSNLRRGAATFILNTVLAP 124
            L+ES AI RY+AT +        PA+  + AKLE  L+    +     +  +   ++ P
Sbjct: 68  VLYESRAINRYIATKYKAEGADLLPAEA-SPAKLEVWLEVESHHFYPAISGLVFQLLIKP 126

Query: 125 SLGLPSSPQAAKEAEKVLFRSLGLIESMWLKGNAKFLLGNPQLSIADLSLVCEIMQLEVL 184
            LG  +   A  E    L + L + ++  L G+ ++L GN + S+AD + +  ++    L
Sbjct: 127 LLGGATDTAAVDEHAAALAQVLDVYDAH-LAGS-RYLAGN-RFSLADANHMSYLL---FL 180

Query: 185 GDSERDRILGPHEKIRSWVQNV 206
             +    ++     +++W  ++
Sbjct: 181 SKTPMAELVASRPHVKAWWDDI 202



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,981,888
Number of extensions: 317404
Number of successful extensions: 727
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 729
Number of HSP's successfully gapped: 4
Length of query: 246
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 148
Effective length of database: 11,918,829
Effective search space: 1763986692
Effective search space used: 1763986692
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 155 (64.3 bits)