BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os11g0284900 Os11g0284900|AK101583
(144 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os11g0284900 Similar to Phospholipid hydroperoxide glutathi... 293 2e-80
Os02g0664000 Similar to Phospholipid hydroperoxide glutathi... 209 6e-55
Os04g0556300 Glutathione peroxidase 209 8e-55
Os03g0358100 Similar to Glutathione peroxidase 162 5e-41
Os06g0185900 Similar to Phospholipid hydroperoxide glutathi... 100 6e-22
>Os11g0284900 Similar to Phospholipid hydroperoxide glutathione peroxidase (EC
1.11.1.9)
Length = 144
Score = 293 bits (751), Expect = 2e-80, Method: Compositional matrix adjust.
Identities = 144/144 (100%), Positives = 144/144 (100%)
Query: 1 CGLYEHLCFITSPLLFCSGLTNSNYKELNVLYEKYKEKGLEILAFPCNQFAGQEPGSNEE 60
CGLYEHLCFITSPLLFCSGLTNSNYKELNVLYEKYKEKGLEILAFPCNQFAGQEPGSNEE
Sbjct: 1 CGLYEHLCFITSPLLFCSGLTNSNYKELNVLYEKYKEKGLEILAFPCNQFAGQEPGSNEE 60
Query: 61 IEQTVCTRFKAEFPIFDKIDVNGKEAAPLYKFLKSQKGGFLGDGIKWNFTKFLVGKDGKV 120
IEQTVCTRFKAEFPIFDKIDVNGKEAAPLYKFLKSQKGGFLGDGIKWNFTKFLVGKDGKV
Sbjct: 61 IEQTVCTRFKAEFPIFDKIDVNGKEAAPLYKFLKSQKGGFLGDGIKWNFTKFLVGKDGKV 120
Query: 121 VERYAPTTSPLKIENDIQKLLGTS 144
VERYAPTTSPLKIENDIQKLLGTS
Sbjct: 121 VERYAPTTSPLKIENDIQKLLGTS 144
>Os02g0664000 Similar to Phospholipid hydroperoxide glutathione peroxidase,
chloroplast precursor (EC 1.11.1.12) (PHGPx)
Length = 238
Score = 209 bits (532), Expect = 6e-55, Method: Compositional matrix adjust.
Identities = 96/126 (76%), Positives = 109/126 (86%)
Query: 19 GLTNSNYKELNVLYEKYKEKGLEILAFPCNQFAGQEPGSNEEIEQTVCTRFKAEFPIFDK 78
GLTNSNY EL+ LYEKYK++G EILAFPCNQF GQEPG+NEEI Q CTRFKAE+PIFDK
Sbjct: 113 GLTNSNYTELSQLYEKYKDQGFEILAFPCNQFGGQEPGTNEEIVQFACTRFKAEYPIFDK 172
Query: 79 IDVNGKEAAPLYKFLKSQKGGFLGDGIKWNFTKFLVGKDGKVVERYAPTTSPLKIENDIQ 138
+DVNG AP+YKFLKS KGG GD IKWNF+KFLV K+G+VVERYAPTTSPL +E DI+
Sbjct: 173 VDVNGDNTAPIYKFLKSSKGGLFGDNIKWNFSKFLVDKEGRVVERYAPTTSPLSMEKDIK 232
Query: 139 KLLGTS 144
KLLG+S
Sbjct: 233 KLLGSS 238
>Os04g0556300 Glutathione peroxidase
Length = 168
Score = 209 bits (531), Expect = 8e-55, Method: Compositional matrix adjust.
Identities = 98/126 (77%), Positives = 109/126 (86%)
Query: 19 GLTNSNYKELNVLYEKYKEKGLEILAFPCNQFAGQEPGSNEEIEQTVCTRFKAEFPIFDK 78
GLTNSNY EL+ LYEKYK +G EILAFPCNQF GQEPGSNEEI Q CTRFKAE+PIFDK
Sbjct: 43 GLTNSNYTELSQLYEKYKVQGFEILAFPCNQFGGQEPGSNEEIVQFACTRFKAEYPIFDK 102
Query: 79 IDVNGKEAAPLYKFLKSQKGGFLGDGIKWNFTKFLVGKDGKVVERYAPTTSPLKIENDIQ 138
+DVNG AAPLYK+LKS KGG GD IKWNF+KFLV K+G+VV+RYAPTTSPL IE DI+
Sbjct: 103 VDVNGNNAAPLYKYLKSNKGGLFGDSIKWNFSKFLVDKEGRVVDRYAPTTSPLSIEKDIK 162
Query: 139 KLLGTS 144
KLLG+S
Sbjct: 163 KLLGSS 168
>Os03g0358100 Similar to Glutathione peroxidase
Length = 169
Score = 162 bits (411), Expect = 5e-41, Method: Compositional matrix adjust.
Identities = 74/123 (60%), Positives = 93/123 (75%)
Query: 19 GLTNSNYKELNVLYEKYKEKGLEILAFPCNQFAGQEPGSNEEIEQTVCTRFKAEFPIFDK 78
G T +NY +L LY+K+++K EILAFPCNQF QEPGS+++I+ CTRFKAE+P+F K
Sbjct: 45 GFTETNYTQLTELYQKHRDKDFEILAFPCNQFLRQEPGSDQQIKDFACTRFKAEYPVFQK 104
Query: 79 IDVNGKEAAPLYKFLKSQKGGFLGDGIKWNFTKFLVGKDGKVVERYAPTTSPLKIENDIQ 138
+ VNG +AAPLYKFLK+ K G G IKWNFTKFL+ K+GKV+ RY+ TSPL E DI
Sbjct: 105 VRVNGPDAAPLYKFLKASKPGLFGSRIKWNFTKFLIDKNGKVINRYSTATSPLSFEKDIL 164
Query: 139 KLL 141
K L
Sbjct: 165 KAL 167
>Os06g0185900 Similar to Phospholipid hydroperoxide glutathione peroxidase,
chloroplast precursor (EC 1.11.1.12) (PHGPx)
Length = 67
Score = 99.8 bits (247), Expect = 6e-22, Method: Compositional matrix adjust.
Identities = 47/65 (72%), Positives = 51/65 (78%)
Query: 79 IDVNGKEAAPLYKFLKSQKGGFLGDGIKWNFTKFLVGKDGKVVERYAPTTSPLKIENDIQ 138
+DVNG AP+YKFLKS GGFLGD +KWNF KFLV K GKVVERY PTTSP +IE DIQ
Sbjct: 3 VDVNGPNTAPIYKFLKSSAGGFLGDLVKWNFEKFLVDKTGKVVERYPPTTSPFQIEKDIQ 62
Query: 139 KLLGT 143
KLL
Sbjct: 63 KLLAA 67
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.140 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,002,220
Number of extensions: 204382
Number of successful extensions: 367
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 367
Number of HSP's successfully gapped: 5
Length of query: 144
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 53
Effective length of database: 12,284,327
Effective search space: 651069331
Effective search space used: 651069331
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 151 (62.8 bits)