BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os11g0245100 Os11g0245100|J065182P11
(104 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os11g0245100 Glutathione S-transferase, N-terminal domain c... 216 3e-57
Os02g0564000 Similar to Glutathione S-transferase 107 2e-24
Os12g0210200 Similar to Glutathione S-transferase GST 18 (E... 87 3e-18
Os12g0210300 Similar to Glutathione S-transferase GST 18 (E... 84 2e-17
AK073415 84 2e-17
>Os11g0245100 Glutathione S-transferase, N-terminal domain containing protein
Length = 104
Score = 216 bits (550), Expect = 3e-57, Method: Compositional matrix adjust.
Identities = 104/104 (100%), Positives = 104/104 (100%)
Query: 1 MASSGSPEARQTHGEIAGAAAPERRLKLYSFWRSSCSYRVRIALSLKGLDYEYKPINLLA 60
MASSGSPEARQTHGEIAGAAAPERRLKLYSFWRSSCSYRVRIALSLKGLDYEYKPINLLA
Sbjct: 1 MASSGSPEARQTHGEIAGAAAPERRLKLYSFWRSSCSYRVRIALSLKGLDYEYKPINLLA 60
Query: 61 NEQSHPEFEKLNPMKYVPALVDGDDTVVVDSFAILLVRGIYMRK 104
NEQSHPEFEKLNPMKYVPALVDGDDTVVVDSFAILLVRGIYMRK
Sbjct: 61 NEQSHPEFEKLNPMKYVPALVDGDDTVVVDSFAILLVRGIYMRK 104
>Os02g0564000 Similar to Glutathione S-transferase
Length = 237
Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust.
Identities = 51/72 (70%), Positives = 61/72 (84%), Gaps = 1/72 (1%)
Query: 25 RLKLYSFWRSSCSYRVRIALSLKGLDYEYKPINLLANEQSHPEFEKLNPMKYVPALVDGD 84
+L LYS+WRSSCS+RVRIAL+LKGL+YEYK +NLL E S PEF K+NPMK+VPALVDG
Sbjct: 13 KLGLYSYWRSSCSHRVRIALNLKGLEYEYKAVNLLKGEHSDPEFMKVNPMKFVPALVDG- 71
Query: 85 DTVVVDSFAILL 96
D V+ DS+AI L
Sbjct: 72 DAVIGDSYAIAL 83
>Os12g0210200 Similar to Glutathione S-transferase GST 18 (EC 2.5.1.18)
Length = 214
Score = 87.0 bits (214), Expect = 3e-18, Method: Compositional matrix adjust.
Identities = 45/78 (57%), Positives = 58/78 (74%), Gaps = 6/78 (7%)
Query: 19 AAAPERRLKLYSFWRSSCSYRVRIALSLKGLDYEYKPINLLANEQSHPEFEKLNPMKYVP 78
AAA + + LYS W SSCSYRVRIAL+LKG+DYEY+ + + P++ K+NP+KYVP
Sbjct: 2 AAAEKTKPVLYSEWMSSCSYRVRIALNLKGIDYEYRAVT-----RGDPDYGKINPIKYVP 56
Query: 79 ALVDGDDTVVVDSFAILL 96
ALVDGD T + DS AI+L
Sbjct: 57 ALVDGDFT-ISDSLAIIL 73
>Os12g0210300 Similar to Glutathione S-transferase GST 18 (EC 2.5.1.18)
Length = 212
Score = 84.3 bits (207), Expect = 2e-17, Method: Compositional matrix adjust.
Identities = 41/69 (59%), Positives = 53/69 (76%), Gaps = 5/69 (7%)
Query: 28 LYSFWRSSCSYRVRIALSLKGLDYEYKPINLLANEQSHPEFEKLNPMKYVPALVDGDDTV 87
LY W SSCS+R+RI L+LKG+DYEYK + N ++ P++EK+NP+KY+PALVDG D V
Sbjct: 8 LYGAWISSCSHRIRIVLNLKGVDYEYKSV----NPRTDPDYEKINPIKYIPALVDG-DLV 62
Query: 88 VVDSFAILL 96
V DS AI L
Sbjct: 63 VSDSLAIAL 71
>AK073415
Length = 212
Score = 84.3 bits (207), Expect = 2e-17, Method: Compositional matrix adjust.
Identities = 41/69 (59%), Positives = 53/69 (76%), Gaps = 5/69 (7%)
Query: 28 LYSFWRSSCSYRVRIALSLKGLDYEYKPINLLANEQSHPEFEKLNPMKYVPALVDGDDTV 87
LY W SSCS+R+RI L+LKG+DYEYK + N ++ P++EK+NP+KY+PALVDG D V
Sbjct: 8 LYGAWISSCSHRIRIVLNLKGVDYEYKSV----NPRTDPDYEKINPIKYIPALVDG-DLV 62
Query: 88 VVDSFAILL 96
V DS AI L
Sbjct: 63 VSDSLAIAL 71
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.135 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,652,382
Number of extensions: 129582
Number of successful extensions: 378
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 376
Number of HSP's successfully gapped: 5
Length of query: 104
Length of database: 17,035,801
Length adjustment: 72
Effective length of query: 32
Effective length of database: 13,276,393
Effective search space: 424844576
Effective search space used: 424844576
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)