BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0143500 Os11g0143500|AK102697
         (583 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0143500  Similar to L-Galactono-1,4-lactone dehydrogenase    991   0.0  
Os12g0139600  Similar to L-Galactono-1,4-lactone dehydrogenase    107   2e-23
Os08g0519100  Plant-specific FAD-dependent oxidoreductase fa...    68   2e-11

>Os11g0143500 Similar to L-Galactono-1,4-lactone dehydrogenase
          Length = 583

 Score =  991 bits (2562), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 482/522 (92%), Positives = 482/522 (92%)

Query: 62  SFPLPPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQXXXXXXXXXXXXXXXG 121
           SFPLPPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQ               G
Sbjct: 62  SFPLPPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQPDSLPALHDALAAAHG 121

Query: 122 ECRKLRPLGSGLSPNGLALSRAGMVNLALMDKVLGVDAKKKTVTVQAGIRVAELVDALRE 181
           ECRKLRPLGSGLSPNGLALSRAGMVNLALMDKVLGVDAKKKTVTVQAGIRVAELVDALRE
Sbjct: 122 ECRKLRPLGSGLSPNGLALSRAGMVNLALMDKVLGVDAKKKTVTVQAGIRVAELVDALRE 181

Query: 182 HGLTLQNFASIREQQVGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPD 241
           HGLTLQNFASIREQQVGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPD
Sbjct: 182 HGLTLQNFASIREQQVGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPD 241

Query: 242 LFYLARCGLGGLGVVAEVTLQCVERHQLIEHTFVSNADEVKKNHKKWLSENKHIKYLWIP 301
           LFYLARCGLGGLGVVAEVTLQCVERHQLIEHTFVSNADEVKKNHKKWLSENKHIKYLWIP
Sbjct: 242 LFYLARCGLGGLGVVAEVTLQCVERHQLIEHTFVSNADEVKKNHKKWLSENKHIKYLWIP 301

Query: 302 YTDTVVVVQCNPPSRWRTPKFTSKYGKDEAIQHVRDLYHESLKKYRTKAESNDPEVDQLS 361
           YTDTVVVVQCNPPSRWRTPKFTSKYGKDEAIQHVRDLYHESLKKYRTKAESNDPEVDQLS
Sbjct: 302 YTDTVVVVQCNPPSRWRTPKFTSKYGKDEAIQHVRDLYHESLKKYRTKAESNDPEVDQLS 361

Query: 362 FTEXXXXXXXXXXXXXXHVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCF 421
           FTE              HVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCF
Sbjct: 362 FTELRDRLLTLDPLDKDHVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCF 421

Query: 422 PAGTLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTACXXXXXXXXXXXQEDDIFSW 481
           PAGTLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTAC           QEDDIFSW
Sbjct: 422 PAGTLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSW 481

Query: 482 VGIIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELAELQA 541
           VGIIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELAELQA
Sbjct: 482 VGIIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELAELQA 541

Query: 542 RLRKRFPVDAYNKARMELDPNKVLSNAKLEKLFPVTEVQHEK 583
           RLRKRFPVDAYNKARMELDPNKVLSNAKLEKLFPVTEVQHEK
Sbjct: 542 RLRKRFPVDAYNKARMELDPNKVLSNAKLEKLFPVTEVQHEK 583



>Os12g0139600 Similar to L-Galactono-1,4-lactone dehydrogenase
          Length = 63

 Score =  107 bits (267), Expect = 2e-23,   Method: Composition-based stats.
 Identities = 48/48 (100%), Positives = 48/48 (100%)

Query: 482 VGIIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEV 529
           VGIIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEV
Sbjct: 11  VGIIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEV 58



>Os08g0519100 Plant-specific FAD-dependent oxidoreductase family protein
          Length = 450

 Score = 68.2 bits (165), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 40/124 (32%), Positives = 73/124 (58%), Gaps = 8/124 (6%)

Query: 145 MVNLALMDKVLGVDAKKKTVTVQAGIRVAELVDALREHGLTLQNFASIREQQVGGIIQVG 204
           +++   +++V+GV+A +  +TV++G+ ++EL+DA    GL L +     +  VGG++  G
Sbjct: 107 VISTNALNRVVGVNAARMEITVESGVTLSELIDAAAHAGLALPHAPYWLDLTVGGLLSTG 166

Query: 205 AHGT--GARLPPIDEQVISMKLVTPAKGT-----IELSREKDPDLFYLARCGLGGLGVVA 257
           AHG+    +   + E V  M++VTPA  +     + +    DPDL  + +  LG LGV++
Sbjct: 167 AHGSSVSGKGGAVHEYVTGMRVVTPAPASEGYAKVRVVNAGDPDLDAV-KVSLGVLGVIS 225

Query: 258 EVTL 261
           +VTL
Sbjct: 226 QVTL 229



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.134    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 17,598,816
Number of extensions: 698090
Number of successful extensions: 1625
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1628
Number of HSP's successfully gapped: 4
Length of query: 583
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 477
Effective length of database: 11,501,117
Effective search space: 5486032809
Effective search space used: 5486032809
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 159 (65.9 bits)