BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0137200 Os11g0137200|J065176G23
         (68 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0137200  Pyruvate carboxyltransferase domain containing...   134   1e-32
Os12g0134000  Similar to Hydroxymethylglutaryl-CoA lyase          120   2e-28
Os01g0269000  Similar to Hydroxymethylglutaryl-CoA lyase           99   7e-22

>Os11g0137200 Pyruvate carboxyltransferase domain containing protein
          Length = 68

 Score =  134 bits (337), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 68/68 (100%), Positives = 68/68 (100%)

Query: 1  ITGFQVLWELPRFVKIVEVGPRDGLQNEKSTVPASVKIELIHKLVASGLSVVEATSFVSP 60
          ITGFQVLWELPRFVKIVEVGPRDGLQNEKSTVPASVKIELIHKLVASGLSVVEATSFVSP
Sbjct: 1  ITGFQVLWELPRFVKIVEVGPRDGLQNEKSTVPASVKIELIHKLVASGLSVVEATSFVSP 60

Query: 61 KWVPQVIT 68
          KWVPQVIT
Sbjct: 61 KWVPQVIT 68



>Os12g0134000 Similar to Hydroxymethylglutaryl-CoA lyase
          Length = 377

 Score =  120 bits (302), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 57/62 (91%), Positives = 60/62 (96%)

Query: 5   QVLWELPRFVKIVEVGPRDGLQNEKSTVPASVKIELIHKLVASGLSVVEATSFVSPKWVP 64
            VLW+LPRFVKIVEVGPRDGLQNEK+TVP SVKIELIHKLVASGLSVVEATSFVSPKWVP
Sbjct: 74  HVLWDLPRFVKIVEVGPRDGLQNEKNTVPTSVKIELIHKLVASGLSVVEATSFVSPKWVP 133

Query: 65  QV 66
           Q+
Sbjct: 134 QL 135



>Os01g0269000 Similar to Hydroxymethylglutaryl-CoA lyase
          Length = 459

 Score = 99.0 bits (245), Expect = 7e-22,   Method: Composition-based stats.
 Identities = 47/62 (75%), Positives = 54/62 (87%)

Query: 5   QVLWELPRFVKIVEVGPRDGLQNEKSTVPASVKIELIHKLVASGLSVVEATSFVSPKWVP 64
           +++ +LPR VKIVEVGPRDGLQNEK+ VP  VKIELI +L  SGLSVVEATSFVSPKWVP
Sbjct: 149 KIIHDLPRSVKIVEVGPRDGLQNEKNIVPTHVKIELIQRLATSGLSVVEATSFVSPKWVP 208

Query: 65  QV 66
           Q+
Sbjct: 209 QL 210



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,177,855
Number of extensions: 61906
Number of successful extensions: 88
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 88
Number of HSP's successfully gapped: 3
Length of query: 68
Length of database: 17,035,801
Length adjustment: 40
Effective length of query: 28
Effective length of database: 14,947,241
Effective search space: 418522748
Effective search space used: 418522748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)