BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0572700 Os10g0572700|Os10g0572700
         (361 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0264500  Similar to CoA-thioester hydrolase CHY1 (3-hyd...   342   3e-94
Os10g0572700  Four-helical cytokine family protein                341   5e-94
Os01g0752200  Enoyl-CoA hydratase/isomerase domain containin...   145   7e-35
Os10g0552900  Enoyl-CoA hydratase/isomerase domain containin...   140   2e-33

>Os12g0264500 Similar to CoA-thioester hydrolase CHY1
           (3-hydroxyisobutyryl-coenzyme A hydrolase)
          Length = 489

 Score =  342 bits (876), Expect = 3e-94,   Method: Compositional matrix adjust.
 Identities = 165/182 (90%), Positives = 170/182 (93%)

Query: 180 EESLKKVDTSDPFAICGIIDQYSQQPSLKEKSALNRLEIINKCFSKRTVEEIIASLEQEA 239
           EESLKKVDTSDPFAICGIIDQYSQQPSLKEKSALNRLEIINKCFSK+TVEEIIASLEQEA
Sbjct: 308 EESLKKVDTSDPFAICGIIDQYSQQPSLKEKSALNRLEIINKCFSKKTVEEIIASLEQEA 367

Query: 240 PNVADEWVASAIQSLRKASPTSLKISLRSIREGRTQTVGECLRREYRMVCHVMRGDFSRD 299
           PNVADEW+ASAIQS+RKASPTS+KISLRSIREGRTQTVGECLRREYRMVCHVMRGDFSRD
Sbjct: 368 PNVADEWIASAIQSMRKASPTSMKISLRSIREGRTQTVGECLRREYRMVCHVMRGDFSRD 427

Query: 300 FFEGCRAILLDKDRNPKWMPPRLEEVHDEVVEKYFSKVXXXXXXXXXXXXRRSHGRRLVP 359
           FFEGCRAILLD+DRNPKWMPPRLEEVHDEVVEKYFSKV            RRSHGRRLVP
Sbjct: 428 FFEGCRAILLDRDRNPKWMPPRLEEVHDEVVEKYFSKVDDPEWEDLDLPPRRSHGRRLVP 487

Query: 360 KL 361
           KL
Sbjct: 488 KL 489



>Os10g0572700 Four-helical cytokine family protein
          Length = 361

 Score =  341 bits (875), Expect = 5e-94,   Method: Compositional matrix adjust.
 Identities = 170/182 (93%), Positives = 170/182 (93%)

Query: 180 EESLKKVDTSDPFAICGIIDQYSQQPSLKEKSALNRLEIINKCFSKRTVEEIIASLEQEA 239
           EESLKKVDTSDPFAICGIIDQYSQQPSLKEKSALNRLEIINKCFSKRTVEEIIASLEQEA
Sbjct: 180 EESLKKVDTSDPFAICGIIDQYSQQPSLKEKSALNRLEIINKCFSKRTVEEIIASLEQEA 239

Query: 240 PNVADEWVASAIQSLRKASPTSLKISLRSIREGRTQTVGECLRREYRMVCHVMRGDFSRD 299
           PNVADEWVASAIQSLRKASPTSLKISLRSIREGRTQTVGECLRREYRMVCHVMRGDFSRD
Sbjct: 240 PNVADEWVASAIQSLRKASPTSLKISLRSIREGRTQTVGECLRREYRMVCHVMRGDFSRD 299

Query: 300 FFEGCRAILLDKDRNPKWMPPRLEEVHDEVVEKYFSKVXXXXXXXXXXXXRRSHGRRLVP 359
           FFEGCRAILLDKDRNPKWMPPRLEEVHDEVVEKYFSKV            RRSHGRRLVP
Sbjct: 300 FFEGCRAILLDKDRNPKWMPPRLEEVHDEVVEKYFSKVDDPEWEDLDLPPRRSHGRRLVP 359

Query: 360 KL 361
           KL
Sbjct: 360 KL 361



>Os01g0752200 Enoyl-CoA hydratase/isomerase domain containing protein
          Length = 389

 Score =  145 bits (365), Expect = 7e-35,   Method: Compositional matrix adjust.
 Identities = 66/155 (42%), Positives = 104/155 (67%)

Query: 180 EESLKKVDTSDPFAICGIIDQYSQQPSLKEKSALNRLEIINKCFSKRTVEEIIASLEQEA 239
           E+ L  ++T D  A+   I+++S      E S LN+L  INKCFS  T+E+II + E E 
Sbjct: 202 EKCLLNLNTGDESAVRAAIEEFSTDVQPDEDSILNKLPTINKCFSAETIEDIIKAFESEG 261

Query: 240 PNVADEWVASAIQSLRKASPTSLKISLRSIREGRTQTVGECLRREYRMVCHVMRGDFSRD 299
               ++W+A+ ++ +R++SPTSLK++LRSIREGR Q++ ECL++E+R+  + +R   + D
Sbjct: 262 SIDGNQWIATVLKGMRRSSPTSLKMTLRSIREGRKQSLPECLKKEFRLTMNTLRSVVTGD 321

Query: 300 FFEGCRAILLDKDRNPKWMPPRLEEVHDEVVEKYF 334
            +EG RA+ +DKD  PKW P  LEEV +E +++ F
Sbjct: 322 VYEGIRALSIDKDNAPKWSPATLEEVKNEDIDRLF 356



>Os10g0552900 Enoyl-CoA hydratase/isomerase domain containing protein
          Length = 411

 Score =  140 bits (353), Expect = 2e-33,   Method: Compositional matrix adjust.
 Identities = 72/158 (45%), Positives = 103/158 (65%)

Query: 180 EESLKKVDTSDPFAICGIIDQYSQQPSLKEKSALNRLEIINKCFSKRTVEEIIASLEQEA 239
           +E L  + T DP  I   +  Y       + S ++RLE+I+KCFS  TVEEI+ ++E EA
Sbjct: 236 DERLATLLTDDPSVIDTSLTHYGDLVYPDKSSIVHRLEVIDKCFSLETVEEIVDAMEIEA 295

Query: 240 PNVADEWVASAIQSLRKASPTSLKISLRSIREGRTQTVGECLRREYRMVCHVMRGDFSRD 299
             + ++W   A++ L++ASP +LK+SLRSIREGR QT+ ECL REYRM  + +   FS +
Sbjct: 296 ARLNEDWSTLALKRLKEASPLALKVSLRSIREGRYQTLDECLVREYRMSINGISEKFSHE 355

Query: 300 FFEGCRAILLDKDRNPKWMPPRLEEVHDEVVEKYFSKV 337
           F EG RA L++KD  PKW PP LE V  ++V+ YF+ +
Sbjct: 356 FREGVRARLVEKDLAPKWDPPALEYVSADMVDSYFAPL 393



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.134    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,017,482
Number of extensions: 207817
Number of successful extensions: 520
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 520
Number of HSP's successfully gapped: 4
Length of query: 361
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 259
Effective length of database: 11,709,973
Effective search space: 3032883007
Effective search space used: 3032883007
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 157 (65.1 bits)