BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os10g0532300 Os10g0532300|AK069934
(179 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os10g0532300 Heavy metal transport/detoxification protein d... 217 5e-57
Os12g0421000 Similar to Barley stem rust resistance protein 105 2e-23
Os03g0126700 Similar to Barley stem rust resistance protein 101 3e-22
>Os10g0532300 Heavy metal transport/detoxification protein domain containing
protein
Length = 179
Score = 217 bits (552), Expect = 5e-57, Method: Compositional matrix adjust.
Identities = 115/179 (64%), Positives = 115/179 (64%)
Query: 1 MSKKIVVKLNVHDKAEKQKAMKAVSALIGIDELSMDMASQKMTVIGMVDPVNVVSKLRKS 60
MSKKIVVKLNVHDKAEKQKAMKAVSALIGIDELSMDMASQKMTVIGMVDPVNVVSKLRKS
Sbjct: 1 MSKKIVVKLNVHDKAEKQKAMKAVSALIGIDELSMDMASQKMTVIGMVDPVNVVSKLRKS 60
Query: 61 WAATIESVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120
WAATIESV
Sbjct: 61 WAATIESVGPAKEPEKKEEKKDGGGDGKKDGGGDGKKEGEAGDKKDGDAAKKDGDKDGEA 120
Query: 121 XXXXXXXXXXXXTEQQLFAELMNQYYHRPAAYGYNPYMSVPPHYVVQSMEENPNSCAIC 179
TEQQLFAELMNQYYHRPAAYGYNPYMSVPPHYVVQSMEENPNSCAIC
Sbjct: 121 KKEDGDKKPAAPTEQQLFAELMNQYYHRPAAYGYNPYMSVPPHYVVQSMEENPNSCAIC 179
>Os12g0421000 Similar to Barley stem rust resistance protein
Length = 239
Score = 105 bits (262), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 55/69 (79%), Positives = 63/69 (91%), Gaps = 1/69 (1%)
Query: 1 MSKKIVVKLNVHDKAEKQKAMKAVSALIGIDELSMDMASQKMTVIGMVDPVNVVSKLRK- 59
MSKKIVVKL++HD +KQKAMKAVS++IGID +SMDMAS+KMTVIG VDPV+VVSKLRK
Sbjct: 1 MSKKIVVKLDLHDNKDKQKAMKAVSSVIGIDAISMDMASRKMTVIGTVDPVDVVSKLRKA 60
Query: 60 SWAATIESV 68
SWAA IESV
Sbjct: 61 SWAAYIESV 69
>Os03g0126700 Similar to Barley stem rust resistance protein
Length = 195
Score = 101 bits (251), Expect = 3e-22, Method: Compositional matrix adjust.
Identities = 53/69 (76%), Positives = 59/69 (85%), Gaps = 1/69 (1%)
Query: 1 MSKKIVVKLNVHDKAEKQKAMKAVSALIGIDELSMDMASQKMTVIGMVDPVNVVSKLRK- 59
MSKKIVVKL++HD +KQKAMK VS L GID +SMDMAS+KMTVIG VDPVNVVSKLRK
Sbjct: 1 MSKKIVVKLDLHDNKDKQKAMKVVSTLAGIDAISMDMASRKMTVIGTVDPVNVVSKLRKA 60
Query: 60 SWAATIESV 68
SW A IES+
Sbjct: 61 SWPAYIESL 69
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.316 0.127 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,465,879
Number of extensions: 81995
Number of successful extensions: 256
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 256
Number of HSP's successfully gapped: 4
Length of query: 179
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 85
Effective length of database: 12,127,685
Effective search space: 1030853225
Effective search space used: 1030853225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 153 (63.5 bits)