BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0455900 Os10g0455900|AK072120
         (315 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0455900  Pleckstrin homology-type domain containing pro...   631   0.0  
Os02g0102800  Pleckstrin homology-type domain containing pro...   341   4e-94
Os06g0116500  Pleckstrin homology-type domain containing pro...   338   3e-93
Os08g0439100  Pleckstrin homology-type domain containing pro...    84   1e-16

>Os10g0455900 Pleckstrin homology-type domain containing protein
          Length = 315

 Score =  631 bits (1628), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 301/308 (97%), Positives = 301/308 (97%)

Query: 8   VVYEGWMVRYGRRKIGRSFIHMRYFVLETRLLSYYKRKPQHKMPKLPIKSLHIDGNCRVE 67
           VVYEGWMVRYGRRKIGRSFIHMRYFVLETRLLSYYKRKPQHKMPKLPIKSLHIDGNCRVE
Sbjct: 8   VVYEGWMVRYGRRKIGRSFIHMRYFVLETRLLSYYKRKPQHKMPKLPIKSLHIDGNCRVE 67

Query: 68  DRGLKMHHGHMLYVLCVYNKREKHQRITMAAFNIQEALIWKEKIEMVIDQQQGVVASDGN 127
           DRGLKMHHGHMLYVLCVYNKREKHQRITMAAFNIQEALIWKEKIEMVIDQQQGVVASDGN
Sbjct: 68  DRGLKMHHGHMLYVLCVYNKREKHQRITMAAFNIQEALIWKEKIEMVIDQQQGVVASDGN 127

Query: 128 LAHSSSQQKVSLENGRKSSFSDHESLYSHXXXXXXXDNQRSLMRRTTIGNGPPESLYDWT 187
           LAHSSSQQKVSLENGRKSSFSDHESLYSH       DNQRSLMRRTTIGNGPPESLYDWT
Sbjct: 128 LAHSSSQQKVSLENGRKSSFSDHESLYSHEEEEEEEDNQRSLMRRTTIGNGPPESLYDWT 187

Query: 188 RENDLGISNQGSPDHVFSRRHWRLVRCQNGLRIFEELQDVDYLARSCSRAMKAVGVVEAS 247
           RENDLGISNQGSPDHVFSRRHWRLVRCQNGLRIFEELQDVDYLARSCSRAMKAVGVVEAS
Sbjct: 188 RENDLGISNQGSPDHVFSRRHWRLVRCQNGLRIFEELQDVDYLARSCSRAMKAVGVVEAS 247

Query: 248 CEAIFQLVMSMDTTRYEWDCSFHWIGSQCLFGLVIFVMYDIGDVMMMGAMLCYFNPESTQ 307
           CEAIFQLVMSMDTTRYEWDCSFHWIGSQCLFGLVIFVMYDIGDVMMMGAMLCYFNPESTQ
Sbjct: 248 CEAIFQLVMSMDTTRYEWDCSFHWIGSQCLFGLVIFVMYDIGDVMMMGAMLCYFNPESTQ 307

Query: 308 TVVHNQDL 315
           TVVHNQDL
Sbjct: 308 TVVHNQDL 315



>Os02g0102800 Pleckstrin homology-type domain containing protein
          Length = 804

 Score =  341 bits (875), Expect = 4e-94,   Method: Compositional matrix adjust.
 Identities = 162/264 (61%), Positives = 204/264 (77%), Gaps = 11/264 (4%)

Query: 8   VVYEGWMVRYGRRKIGRSFIHMRYFVLETRLLSYYKRKPQHKMPKLPIKSLHIDGNCRVE 67
           V +EGWMVRYGRRKIGRSF H RYFVL++RLL+YYK+KP+  M  +P+KSL IDGNCRVE
Sbjct: 71  VHHEGWMVRYGRRKIGRSFFHTRYFVLDSRLLAYYKKKPKDNM--VPLKSLLIDGNCRVE 128

Query: 68  DRGLKMHHGHMLYVLCVYNKREKHQRITMAAFNIQEALIWKEKIEMVIDQQQGVVASDGN 127
           DRGLK HHG M+YVLCVYNK+EK  +ITM A++I++AL WK+ IE++IDQQ+ + + +  
Sbjct: 129 DRGLKTHHGQMVYVLCVYNKKEKEHQITMGAYDIEDALAWKKNIELIIDQQENMTSKN-- 186

Query: 128 LAHSSSQQKVSLENGRKSSFSDHESLYSHXXXXXXXDNQRSLMRRTTIGNGPPESLYDWT 187
              + +      E G +  FSDH+S           + +  L+RRTTIGNGPPES++DWT
Sbjct: 187 -RKAFASMDFDTELGGQFIFSDHDS------AAEDEEERPMLIRRTTIGNGPPESIHDWT 239

Query: 188 RENDLGISNQGSPDHVFSRRHWRLVRCQNGLRIFEELQDVDYLARSCSRAMKAVGVVEAS 247
           +E+D+G  NQ  P  V S+++WRL+RCQNGLRIFEEL + DYLARSCSRAM+AVGVVEA+
Sbjct: 240 KEHDIGPPNQIDPIQVSSKKNWRLLRCQNGLRIFEELLEFDYLARSCSRAMRAVGVVEAT 299

Query: 248 CEAIFQLVMSMDTTRYEWDCSFHW 271
           CEAIF LVMSMD TRYEWDCSF +
Sbjct: 300 CEAIFGLVMSMDVTRYEWDCSFRY 323



>Os06g0116500 Pleckstrin homology-type domain containing protein
          Length = 366

 Score =  338 bits (867), Expect = 3e-93,   Method: Compositional matrix adjust.
 Identities = 156/264 (59%), Positives = 207/264 (78%), Gaps = 9/264 (3%)

Query: 8   VVYEGWMVRYGRRKIGRSFIHMRYFVLETRLLSYYKRKPQHKMPKLPIKSLHIDGNCRVE 67
           V +EGWM+RYGRRKIGRSF+  RYFVL+ +LL+YYK++P+  M  +P+K+L IDGNCRVE
Sbjct: 52  VRHEGWMLRYGRRKIGRSFVRTRYFVLDNKLLAYYKKQPKDNM--VPVKALQIDGNCRVE 109

Query: 68  DRGLKMHHGHMLYVLCVYNKREKHQRITMAAFNIQEALIWKEKIEMVIDQQQGVVASDGN 127
           DRGLK HHG M+YVLC+YNK+EK   ITM A +I++AL+WK+K+E++IDQQQ  + +   
Sbjct: 110 DRGLKTHHGQMVYVLCIYNKKEKENHITMGAHDIEDALVWKKKLELLIDQQQDTMTAKNR 169

Query: 128 LAHSSSQQKVSLENGRKSSFSDHESLYSHXXXXXXXDNQRSLMRRTTIGNGPPESLYDWT 187
            A +S      +E G   SFSD +S           + + +L+RRTTIGNGPP+S++DWT
Sbjct: 170 KAFAS--LDFDMEFGGPLSFSDRDS-----GPEDEEEPRPTLLRRTTIGNGPPDSVHDWT 222

Query: 188 RENDLGISNQGSPDHVFSRRHWRLVRCQNGLRIFEELQDVDYLARSCSRAMKAVGVVEAS 247
           +E D+G+S+Q   +H +SR++WRL+RCQNGLRIFEEL +V+YLARSCSRAM+AVGVVEA+
Sbjct: 223 KEPDIGLSDQNDTNHAYSRKNWRLLRCQNGLRIFEELVEVEYLARSCSRAMRAVGVVEAT 282

Query: 248 CEAIFQLVMSMDTTRYEWDCSFHW 271
           CE+IF L+MSMD TRYEWDCSF +
Sbjct: 283 CESIFGLIMSMDVTRYEWDCSFQY 306



>Os08g0439100 Pleckstrin homology-type domain containing protein
          Length = 763

 Score = 84.0 bits (206), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 68/259 (26%), Positives = 112/259 (43%), Gaps = 24/259 (9%)

Query: 13  WMVRYGRRKIGRSFIHMRYFVLETRLLSYYKRKPQHKMPKLPIKSLHIDGNCRVEDRGLK 72
           W+   G   IG  + H+R+ V+  + ++ YKR P       PI+   +     VE+ G +
Sbjct: 52  WVYHLGVNSIGHEYRHLRFLVIRGKTVAMYKRDPSKNPGIQPIRKGVVSHTLMVEELGRR 111

Query: 73  MHHGHMLYVLCVYNKREKHQRITMAAFNIQEALIWKEKIEMVIDQQQGVVASDG-NLAHS 131
           +     LYVL  YN+ ++ ++  +A  +  EA  W E  E    Q    + + G +   S
Sbjct: 112 ITSHGELYVLRFYNRLDQTKKGEIACGDPGEARKWVEAFEQAKQQADYDLMTRGVSWNRS 171

Query: 132 SSQQKVSLENGRKSSFSDHESLYSHXXXXXXXDNQRSLMRRTTIGNGPPESLYDWTREND 191
            ++ +++L+  R                       + L +   IG GP + L   +    
Sbjct: 172 QNENELNLDGHRP----------------RVRRYAQGLGKLVRIGKGPEKLLRQSSNLQS 215

Query: 192 LGISNQ---GSPDHVFSRRHWRLVRCQNGLRIFEELQDVDYLARSCSRAMKAVGVVEASC 248
             I N    G     F    WR VR  NG+RIFE++ +           +K+VGVV A+ 
Sbjct: 216 HEIINTNFGGDSGDAFEAHEWRYVRTFNGIRIFEDIANT---KGGKGVLLKSVGVVGANP 272

Query: 249 EAIFQLVMSMDT-TRYEWD 266
           + +F +V+S D   RYEWD
Sbjct: 273 DTVFAVVLSSDKHKRYEWD 291



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.136    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,157,837
Number of extensions: 394909
Number of successful extensions: 997
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 992
Number of HSP's successfully gapped: 4
Length of query: 315
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 214
Effective length of database: 11,762,187
Effective search space: 2517108018
Effective search space used: 2517108018
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 156 (64.7 bits)