BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0422600 Os10g0422600|AK109893
         (345 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0422600  Protein of unknown function DUF581 family protein   500   e-142
Os03g0665200  Protein of unknown function DUF581 family protein   112   3e-25
Os01g0593200  Protein of unknown function DUF581 family protein    73   3e-13
Os01g0719000  Protein of unknown function DUF581 family protein    65   6e-11

>Os10g0422600 Protein of unknown function DUF581 family protein
          Length = 345

 Score =  500 bits (1287), Expect = e-142,   Method: Compositional matrix adjust.
 Identities = 256/345 (74%), Positives = 256/345 (74%)

Query: 1   LATHTVHAADLLRKLKITXXXXXXXXXXXXRIIAVVIIDFDASISMLRKRAKPSSSSGGS 60
           LATHTVHAADLLRKLKIT            RIIAVVIIDFDASISMLRKRAKPSSSSGGS
Sbjct: 1   LATHTVHAADLLRKLKITASSACLASLASCRIIAVVIIDFDASISMLRKRAKPSSSSGGS 60

Query: 61  NKQQHEIAFDHXXXXXXXXXISAPKLLVPSSSGEASPEAVLMSPTSTLQTASATSPCSGR 120
           NKQQHEIAFDH         ISAPKLLVPSSSGEASPEAVLMSPTSTLQTASATSPCSGR
Sbjct: 61  NKQQHEIAFDHGGGGGGAAAISAPKLLVPSSSGEASPEAVLMSPTSTLQTASATSPCSGR 120

Query: 121 TTGAAATAVPFSXXXXXXXXXXXXXXXXXXXQCRTHRPWDATRPVGLGLVGALNXXXXXX 180
           TTGAAATAVPFS                   QCRTHRPWDATRPVGLGLVGALN      
Sbjct: 121 TTGAAATAVPFSRRRRHGGGGVGRGPADGDGQCRTHRPWDATRPVGLGLVGALNDDEDDD 180

Query: 181 XXXXXXXXXXXXXXXXLTGQIRLRVQTNYYAPCTEFGVKSGGAAVQYAXXXXXXXXXXXX 240
                           LTGQIRLRVQTNYYAPCTEFGVKSGGAAVQYA            
Sbjct: 181 DGEVDAAPGDAAASSVLTGQIRLRVQTNYYAPCTEFGVKSGGAAVQYAPPPRRRWMSPRE 240

Query: 241 XXXXXXXDYTCVIARGANPRTTHIFDNRVVESSGDGEYFPPELWPPSAAGKXXXXXXXXF 300
                  DYTCVIARGANPRTTHIFDNRVVESSGDGEYFPPELWPPSAAGK        F
Sbjct: 241 MMEEMSEDYTCVIARGANPRTTHIFDNRVVESSGDGEYFPPELWPPSAAGKGDDDGDGDF 300

Query: 301 LRYCHGCSKDLGLGKDIFMYRGEKAFCSHECRYHEMLFDEGIEEL 345
           LRYCHGCSKDLGLGKDIFMYRGEKAFCSHECRYHEMLFDEGIEEL
Sbjct: 301 LRYCHGCSKDLGLGKDIFMYRGEKAFCSHECRYHEMLFDEGIEEL 345



>Os03g0665200 Protein of unknown function DUF581 family protein
          Length = 302

 Score =  112 bits (281), Expect = 3e-25,   Method: Compositional matrix adjust.
 Identities = 53/98 (54%), Positives = 64/98 (65%), Gaps = 2/98 (2%)

Query: 248 DYTCVIARGANPRTTHIFDNRVVESSGDGEYFPPELWPPSAAGKXXXXXXXXFLRYCHGC 307
           DYTCVI+RG NPRTTHIFD+ +VES GD      ++      G         FL  C+ C
Sbjct: 206 DYTCVISRGPNPRTTHIFDDCIVESCGD--VLVEKVAGGGGDGDGDAVRTNGFLNSCYAC 263

Query: 308 SKDLGLGKDIFMYRGEKAFCSHECRYHEMLFDEGIEEL 345
           +K LG G DIF+YRG+KAFCS ECRY EMLFDE ++ L
Sbjct: 264 NKQLGHGNDIFIYRGDKAFCSSECRYQEMLFDEAVDNL 301



>Os01g0593200 Protein of unknown function DUF581 family protein
          Length = 260

 Score = 72.8 bits (177), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 38/99 (38%), Positives = 56/99 (56%), Gaps = 10/99 (10%)

Query: 248 DYTCVIARGANPRTTHIFDNRVVESSGDGEYFPPELWPPSAAGKXXX--XXXXXFLRYCH 305
           DYT V+ RG NPRTTH F + V+E  G+      +L P  +  +           + +C+
Sbjct: 146 DYTRVVCRGPNPRTTHFFGDHVLEFEGE------QLMPDESKSEESLPPRLEEGMMSFCY 199

Query: 306 GCSKDLGLGKDIFMYRGEKAFCSHECRYHEMLFDEGIEE 344
            C + L  GKDI++Y+G+KAFCS ECR  E   ++ +EE
Sbjct: 200 FCGEKLEEGKDIYVYQGDKAFCSMECR--ENFMEDEMEE 236



>Os01g0719000 Protein of unknown function DUF581 family protein
          Length = 262

 Score = 65.5 bits (158), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 35/86 (40%), Positives = 44/86 (51%), Gaps = 1/86 (1%)

Query: 248 DYTCVIARGANPRTTHIFDNRVVE-SSGDGEYFPPELWPPSAAGKXXXXXXXXFLRYCHG 306
           +YTCVI  G NPRTTHI  +  +E   G        ++     G+          R C  
Sbjct: 149 EYTCVIEHGPNPRTTHILGDETLEVCKGVPRSSKKSIFTIEPIGELPSTLACAVSRSCCY 208

Query: 307 CSKDLGLGKDIFMYRGEKAFCSHECR 332
           C K L   +DI+MY GEKAFCS+ECR
Sbjct: 209 CRKRLQQDRDIYMYLGEKAFCSNECR 234



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.133    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,555,191
Number of extensions: 315681
Number of successful extensions: 1051
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1046
Number of HSP's successfully gapped: 4
Length of query: 345
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 243
Effective length of database: 11,709,973
Effective search space: 2845523439
Effective search space used: 2845523439
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)