BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0521400 Os09g0521400|AK101064
         (476 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0521400  Similar to HMG-CoA synthase                         937   0.0  
Os08g0544900  Similar to HMG-CoA synthase                         818   0.0  
Os03g0118800  Similar to HMG-CoA synthase                         778   0.0  

>Os09g0521400 Similar to HMG-CoA synthase
          Length = 476

 Score =  937 bits (2423), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 450/476 (94%), Positives = 450/476 (94%)

Query: 1   MDGRKDVGILAMDIYFPPTCVLQESLEAHDGASKGKYTIGLGQDCMAFCSEVEDVISMSM 60
           MDGRKDVGILAMDIYFPPTCVLQESLEAHDGASKGKYTIGLGQDCMAFCSEVEDVISMSM
Sbjct: 1   MDGRKDVGILAMDIYFPPTCVLQESLEAHDGASKGKYTIGLGQDCMAFCSEVEDVISMSM 60

Query: 61  TVVTSLLKKYKVDPKLIGRLEVGSETVIDKSKSIKTWLMQIFEECGNTDIEGVDSSNACY 120
           TVVTSLLKKYKVDPKLIGRLEVGSETVIDKSKSIKTWLMQIFEECGNTDIEGVDSSNACY
Sbjct: 61  TVVTSLLKKYKVDPKLIGRLEVGSETVIDKSKSIKTWLMQIFEECGNTDIEGVDSSNACY 120

Query: 121 GGTAALLNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPIAFE 180
           GGTAALLNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPIAFE
Sbjct: 121 GGTAALLNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPIAFE 180

Query: 181 SKYKASHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYRQFCKKYEKLAGKQFSI 240
           SKYKASHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYRQFCKKYEKLAGKQFSI
Sbjct: 181 SKYKASHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYRQFCKKYEKLAGKQFSI 240

Query: 241 SDADYFVFHSPYNKLVQKSFARLYYNDFTRDCSSVDNDAKEKLQPFSNLTGEESYQSRDL 300
           SDADYFVFHSPYNKLVQKSFARLYYNDFTRDCSSVDNDAKEKLQPFSNLTGEESYQSRDL
Sbjct: 241 SDADYFVFHSPYNKLVQKSFARLYYNDFTRDCSSVDNDAKEKLQPFSNLTGEESYQSRDL 300

Query: 301 EKASQQVAKPLYDIKVQPSTLLPKQIGNMYTXXXXXXXXXXXXNKHASLDDQRIVMFSYG 360
           EKASQQVAKPLYDIKVQPSTLLPKQIGNMYT            NKHASLDDQRIVMFSYG
Sbjct: 301 EKASQQVAKPLYDIKVQPSTLLPKQIGNMYTASLYAALASVLYNKHASLDDQRIVMFSYG 360

Query: 361 SGLTSTMFSLKLNNGQDPFSLSNIASVLNATEKLESRHMTLPEKFVETLKLMEHRYGAKD 420
           SGLTSTMFSLKLNNGQDPFSLSNIASVLNATEKLESRHMTLPEKFVETLKLMEHRYGAKD
Sbjct: 361 SGLTSTMFSLKLNNGQDPFSLSNIASVLNATEKLESRHMTLPEKFVETLKLMEHRYGAKD 420

Query: 421 FETCKDTSLLPPGTFYLTRVDSMYRRFYXXXXXXXXXXXXXXYSNGHATNGYANGH 476
           FETCKDTSLLPPGTFYLTRVDSMYRRFY              YSNGHATNGYANGH
Sbjct: 421 FETCKDTSLLPPGTFYLTRVDSMYRRFYERKADEEIAAAKAKYSNGHATNGYANGH 476



>Os08g0544900 Similar to HMG-CoA synthase
          Length = 467

 Score =  818 bits (2113), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 394/476 (82%), Positives = 417/476 (87%), Gaps = 9/476 (1%)

Query: 1   MDGRKDVGILAMDIYFPPTCVLQESLEAHDGASKGKYTIGLGQDCMAFCSEVEDVISMSM 60
           M+   DVGILAMDIYFPP CV QE+LEAHDGASKGKYTIGLGQDCMA+CSEVEDVISMS+
Sbjct: 1   MEQPNDVGILAMDIYFPPACVHQEALEAHDGASKGKYTIGLGQDCMAYCSEVEDVISMSL 60

Query: 61  TVVTSLLKKYKVDPKLIGRLEVGSETVIDKSKSIKTWLMQIFEECGNTDIEGVDSSNACY 120
           TVV SLLKKY +DPK IGRLEVGSETVIDKSKSIKTWLM IFEE GNTDIEGVDSSNACY
Sbjct: 61  TVVASLLKKYNIDPKQIGRLEVGSETVIDKSKSIKTWLMHIFEESGNTDIEGVDSSNACY 120

Query: 121 GGTAALLNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPIAFE 180
           GGTAALLNCVNWVES SWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPI+FE
Sbjct: 121 GGTAALLNCVNWVESKSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPISFE 180

Query: 181 SKYKASHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYRQFCKKYEKLAGKQFSI 240
           SKY+ASHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYRQ+C KYEK+ G+QFSI
Sbjct: 181 SKYRASHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYRQYCTKYEKIVGEQFSI 240

Query: 241 SDADYFVFHSPYNKLVQKSFARLYYNDFTRDCSSVDNDAKEKLQPFSNLTGEESYQSRDL 300
           SDA+Y VFHSPYNKLVQKSFARLY+NDF R+CS+VDNDAKEKLQPF+NLT EESYQSRDL
Sbjct: 241 SDAEYCVFHSPYNKLVQKSFARLYFNDFMRNCSTVDNDAKEKLQPFANLTSEESYQSRDL 300

Query: 301 EKASQQVAKPLYDIKVQPSTLLPKQIGNMYTXXXXXXXXXXXXNKHASLDDQRIVMFSYG 360
           EKASQQVAK LYDIKVQPSTLLPKQIGNMYT            NKH SLD QRI+MFSYG
Sbjct: 301 EKASQQVAKHLYDIKVQPSTLLPKQIGNMYTASLYAALASVIYNKHDSLDGQRILMFSYG 360

Query: 361 SGLTSTMFSLKLNNGQDPFSLSNIASVLNATEKLESRHMTLPEKFVETLKLMEHRYGAKD 420
           SGLTSTMFSL+L++GQ PFSL NIASVL+ T KLESRH+T PEKF+ETLKLMEHRYGAKD
Sbjct: 361 SGLTSTMFSLRLHDGQHPFSLMNIASVLDVTTKLESRHVTSPEKFIETLKLMEHRYGAKD 420

Query: 421 FETCKDTSLLPPGTFYLTRVDSMYRRFYXXXXXXXXXXXXXXYSNGHATNGYANGH 476
           FET KDTSLLPPGTFYLT+VDSMYRRFY                     NG ANGH
Sbjct: 421 FETSKDTSLLPPGTFYLTKVDSMYRRFYDKKAAEGKIK---------GCNGIANGH 467



>Os03g0118800 Similar to HMG-CoA synthase
          Length = 463

 Score =  778 bits (2010), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 371/473 (78%), Positives = 401/473 (84%), Gaps = 14/473 (2%)

Query: 4   RKDVGILAMDIYFPPTCVLQESLEAHDGASKGKYTIGLGQDCMAFCSEVEDVISMSMTVV 63
           RKDVGILAMDIYFPPTCVLQ+ LE HDG SKGKYTIGLGQD MAFC+EVEDVISMS+TVV
Sbjct: 5   RKDVGILAMDIYFPPTCVLQDELENHDGVSKGKYTIGLGQDSMAFCTEVEDVISMSLTVV 64

Query: 64  TSLLKKYKVDPKLIGRLEVGSETVIDKSKSIKTWLMQIFEECGNTDIEGVDSSNACYGGT 123
            SLL+ YK+DPK IGRLEVGSETVIDKSKSIKTWLMQIFEECGNTDIEGVDSSNACYGGT
Sbjct: 65  KSLLENYKIDPKCIGRLEVGSETVIDKSKSIKTWLMQIFEECGNTDIEGVDSSNACYGGT 124

Query: 124 AALLNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPIAFESKY 183
           AAL NCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAP++FESKY
Sbjct: 125 AALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPNAPVSFESKY 184

Query: 184 KASHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYRQFCKKYEKLAGKQFSISDA 243
           + SHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCY  FCKKYEKL GKQFSI DA
Sbjct: 185 RGSHMAHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYNVFCKKYEKLEGKQFSIHDA 244

Query: 244 DYFVFHSPYNKLVQKSFARLYYNDFTRDCSSVDNDAKEKLQPFSNLTGEESYQSRDLEKA 303
           DYFVFHSPYNKLVQKSFARLYYNDF R CS+V++ ++EKL+P+S L+ EESYQSR+LEKA
Sbjct: 245 DYFVFHSPYNKLVQKSFARLYYNDFLRKCSTVEDGSREKLEPYSGLSSEESYQSRELEKA 304

Query: 304 SQQVAKPLYDIKVQPSTLLPKQIGNMYTXXXXXXXXXXXXNKHASLDDQRIVMFSYGSGL 363
           SQQVAK LYD KVQP TL+PKQ+GNMYT            NK+ +L  QRIVMFSYGSGL
Sbjct: 305 SQQVAKHLYDSKVQPITLIPKQVGNMYTASLYAALASVMHNKNETLAGQRIVMFSYGSGL 364

Query: 364 TSTMFSLKLNNGQDPFSLSNIASVLNATEKLESRHMTLPEKFVETLKLMEHRYGAKDFET 423
           TSTMFS K+N GQ PF LSNIA +L+ ++KLESRH+  PEKFV  LKLMEHRYGAKDF T
Sbjct: 365 TSTMFSFKINEGQHPFILSNIAGILDVSKKLESRHVVAPEKFVAALKLMEHRYGAKDFTT 424

Query: 424 CKDTSLLPPGTFYLTRVDSMYRRFYXXXXXXXXXXXXXXYSNGHATNGYANGH 476
            +DTSLL PGT+YLT VDSMYRRFY                 G A    +NGH
Sbjct: 425 SQDTSLLAPGTYYLTHVDSMYRRFYAV--------------KGQAVTEVSNGH 463



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.133    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,315,076
Number of extensions: 596280
Number of successful extensions: 1291
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1288
Number of HSP's successfully gapped: 3
Length of query: 476
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 371
Effective length of database: 11,553,331
Effective search space: 4286285801
Effective search space used: 4286285801
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)