BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0458800 Os09g0458800|009-148-H10
         (105 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0458800  Calcium-binding EF-hand domain containing protein   211   7e-56
Os09g0458900  Calcium-binding EF-hand domain containing protein    94   2e-20
Os07g0619200  Calcium-binding EF-hand domain containing protein    83   4e-17
Os06g0213300                                                       69   1e-12
Os02g0762900  EF-Hand type domain containing protein               67   2e-12

>Os09g0458800 Calcium-binding EF-hand domain containing protein
          Length = 105

 Score =  211 bits (538), Expect = 7e-56,   Method: Compositional matrix adjust.
 Identities = 105/105 (100%), Positives = 105/105 (100%)

Query: 1   MACFGFDLSARARGIGDVYHPFNETAVREKFKEWIRQFDTDGDGRISRDELRRAMRAVGV 60
           MACFGFDLSARARGIGDVYHPFNETAVREKFKEWIRQFDTDGDGRISRDELRRAMRAVGV
Sbjct: 1   MACFGFDLSARARGIGDVYHPFNETAVREKFKEWIRQFDTDGDGRISRDELRRAMRAVGV 60

Query: 61  RFTGIKCRRGMSHADADGDGYIDDSEIDGLIEYWGRRLGLGVAAY 105
           RFTGIKCRRGMSHADADGDGYIDDSEIDGLIEYWGRRLGLGVAAY
Sbjct: 61  RFTGIKCRRGMSHADADGDGYIDDSEIDGLIEYWGRRLGLGVAAY 105



>Os09g0458900 Calcium-binding EF-hand domain containing protein
          Length = 98

 Score = 94.4 bits (233), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 54/98 (55%), Positives = 71/98 (72%)

Query: 8   LSARARGIGDVYHPFNETAVREKFKEWIRQFDTDGDGRISRDELRRAMRAVGVRFTGIKC 67
           ++ R+  +       +     ++FKEW+R+FD D DGRISRDELRRAMRA+  RFTG + 
Sbjct: 1   MAIRSMTVATTTRSLDGDMTVDEFKEWLRRFDVDRDGRISRDELRRAMRAIRARFTGWRS 60

Query: 68  RRGMSHADADGDGYIDDSEIDGLIEYWGRRLGLGVAAY 105
           R+G+S+ADADGDGYIDD E+DGLIEY  + LGL + AY
Sbjct: 61  RQGISYADADGDGYIDDGEVDGLIEYAQKSLGLRIVAY 98



>Os07g0619200 Calcium-binding EF-hand domain containing protein
          Length = 103

 Score = 83.2 bits (204), Expect = 4e-17,   Method: Compositional matrix adjust.
 Identities = 39/85 (45%), Positives = 56/85 (65%)

Query: 21  PFNETAVREKFKEWIRQFDTDGDGRISRDELRRAMRAVGVRFTGIKCRRGMSHADADGDG 80
           P +     + FK W++QFDTD DGRISR ELR A+R  G  F+G+K  R + HAD D  G
Sbjct: 19  PSSREMTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWFSGLKAGRAVRHADRDNSG 78

Query: 81  YIDDSEIDGLIEYWGRRLGLGVAAY 105
           ++D+SEI+ L+ +  + LG+ V A+
Sbjct: 79  FVDESEIENLVAFAQKTLGMKVTAW 103



>Os06g0213300 
          Length = 99

 Score = 68.6 bits (166), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 38/84 (45%), Positives = 53/84 (63%), Gaps = 2/84 (2%)

Query: 21  PFNETAVREKFKEWIRQFDTDGDGRISRDELRRAMRAVGVRFTGIKCRRGMSHADADGDG 80
           P  E  V E+F+ W+ QFD DGDGRISRDEL+RA+R++ + F   K R G+  ADA+ DG
Sbjct: 10  PQGEVTV-EEFRAWLGQFDADGDGRISRDELQRALRSLNLWFAWWKARAGVRAADANRDG 68

Query: 81  YID-DSEIDGLIEYWGRRLGLGVA 103
            +  D E+  L  +  R L + +A
Sbjct: 69  AVAGDDEVATLFAFAQRHLNVKIA 92



>Os02g0762900 EF-Hand type domain containing protein
          Length = 100

 Score = 67.4 bits (163), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 37/87 (42%), Positives = 52/87 (59%), Gaps = 4/87 (4%)

Query: 19  YH---PFNETAVREKFKEWIRQFDTDGDGRISRDELRRAMRAVGVRFTGIKCRRGMSHAD 75
           YH   P  ET V E+F+ W+ QFD +GDGRI R+EL RA+R++ + F   K R  M  AD
Sbjct: 6   YHRALPQGETTV-EEFRAWLSQFDANGDGRIGREELERALRSLNLWFAWWKAREAMREAD 64

Query: 76  ADGDGYIDDSEIDGLIEYWGRRLGLGV 102
           A+ +G +D  E+  L  +  R L L +
Sbjct: 65  ANRNGVVDRDEMVRLYAFAQRHLHLKM 91



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.144    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,175,035
Number of extensions: 182505
Number of successful extensions: 1243
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 1247
Number of HSP's successfully gapped: 5
Length of query: 105
Length of database: 17,035,801
Length adjustment: 73
Effective length of query: 32
Effective length of database: 13,224,179
Effective search space: 423173728
Effective search space used: 423173728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)