BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0424200 Os09g0424200|AK107431
         (272 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0424200  Glutamine amidotransferase class-I domain cont...   475   e-134
Os02g0179200  Glutamine amidotransferase class-I domain cont...   273   7e-74
Os03g0685300  Glutamine amidotransferase class-I domain cont...   222   2e-58

>Os09g0424200 Glutamine amidotransferase class-I domain containing protein
          Length = 272

 Score =  475 bits (1223), Expect = e-134,   Method: Compositional matrix adjust.
 Identities = 236/247 (95%), Positives = 236/247 (95%)

Query: 26  DSDYVRKVYGGYLEVFIRAFXXXXXXXXXXXEEWDMFRAVDGELPGADEVDGYDGFVISG 85
           DSDYVRKVYGGYLEVFIRAF           EEWDMFRAVDGELPGADEVDGYDGFVISG
Sbjct: 26  DSDYVRKVYGGYLEVFIRAFGDDGDVGDGGGEEWDMFRAVDGELPGADEVDGYDGFVISG 85

Query: 86  SPHDAYADDLWILRLCLLVRDLVAMRKRLLGICFGHQVICRALGGRVGKARGGWDIGIRE 145
           SPHDAYADDLWILRLCLLVRDLVAMRKRLLGICFGHQVICRALGGRVGKARGGWDIGIRE
Sbjct: 86  SPHDAYADDLWILRLCLLVRDLVAMRKRLLGICFGHQVICRALGGRVGKARGGWDIGIRE 145

Query: 146 VAMAESLPPYRFLDDALQGITAAAAPYAKITEVHQDEVWELPAGAEVLASSSKTGVEMFC 205
           VAMAESLPPYRFLDDALQGITAAAAPYAKITEVHQDEVWELPAGAEVLASSSKTGVEMFC
Sbjct: 146 VAMAESLPPYRFLDDALQGITAAAAPYAKITEVHQDEVWELPAGAEVLASSSKTGVEMFC 205

Query: 206 AGDRVLGIQGHPEYTADILLNLVDRLSSAGSITMAVAEGVRRQLEDTGPDREFWIKLCKS 265
           AGDRVLGIQGHPEYTADILLNLVDRLSSAGSITMAVAEGVRRQLEDTGPDREFWIKLCKS
Sbjct: 206 AGDRVLGIQGHPEYTADILLNLVDRLSSAGSITMAVAEGVRRQLEDTGPDREFWIKLCKS 265

Query: 266 FLKTEEE 272
           FLKTEEE
Sbjct: 266 FLKTEEE 272



>Os02g0179200 Glutamine amidotransferase class-I domain containing protein
          Length = 299

 Score =  273 bits (699), Expect = 7e-74,   Method: Compositional matrix adjust.
 Identities = 138/243 (56%), Positives = 166/243 (68%), Gaps = 7/243 (2%)

Query: 26  DSDYVRKVYGGYLEVFIRAFXXXXXXXXXXXEEWDMFRAVDGELPGADEVDGYDGFVISG 85
           DS+Y RKVYGGY  VF+ A            E WD FR +DGE P A+EV  Y+GFV+SG
Sbjct: 25  DSEYARKVYGGYGNVFVSALGGGGGGGEE--ERWDCFRVIDGEFPAAEEVGRYEGFVVSG 82

Query: 86  SPHDAYADDLWILRLCLLVRDLVAMRKRLLGICFGHQVICRALGGRVGKARGGWDIGIRE 145
           SPHDAY D+ WILRLC L+R L AM KR+LGICFGHQV+CRALGGR+GKAR GW+IG+++
Sbjct: 83  SPHDAYGDERWILRLCSLLRALHAMGKRILGICFGHQVLCRALGGRIGKARSGWNIGVKK 142

Query: 146 VAMAESLPPYRFLDDALQGITAAAAPYAKITEVHQDEVWELPAGAEVLASSSKTGVEMFC 205
           +         +   D L+ I  +A+    I EVHQDEV E+P    VLA S KT VEMF 
Sbjct: 143 MTFVRDFEGSKLFGD-LKEIPQSAS----IIEVHQDEVLEVPPMGRVLAYSDKTPVEMFA 197

Query: 206 AGDRVLGIQGHPEYTADILLNLVDRLSSAGSITMAVAEGVRRQLEDTGPDREFWIKLCKS 265
            GD VLGIQGHPEYT+DILLNL+DRL +  +IT  + E  RR +E + PDR FW  LCK 
Sbjct: 198 VGDNVLGIQGHPEYTSDILLNLIDRLVNNNTITSGIGEEARRTVEASEPDRRFWTGLCKG 257

Query: 266 FLK 268
           FLK
Sbjct: 258 FLK 260



>Os03g0685300 Glutamine amidotransferase class-I domain containing protein
          Length = 293

 Score =  222 bits (566), Expect = 2e-58,   Method: Compositional matrix adjust.
 Identities = 120/247 (48%), Positives = 151/247 (61%), Gaps = 23/247 (9%)

Query: 26  DSDYVRKVYGGYLEVFIRAFXXXXXXXXXXXEEWDMFRAVDGELPGADEVDGYDGFVISG 85
           DS+YVRK YGGY EVF RA            E W ++RAV GELPG +E  G DGFVISG
Sbjct: 41  DSEYVRKAYGGYFEVF-RALLAEDG------ERWRVYRAVRGELPGEEEAAGIDGFVISG 93

Query: 86  SPHDAYADDLWILRLCLLVRDLVAMRKRLLGICFGHQVICRALGGRVGKARGGWDIGIRE 145
           S  DA+ADD WI+ L  L+R   A  KR+LG+CFGHQV+CRALGG+ G+++ GWDIG+  
Sbjct: 94  SCSDAHADDPWIVALVDLIRRQNAAGKRILGVCFGHQVLCRALGGKTGRSKKGWDIGVNC 153

Query: 146 V----AMAESLPPYRFLDDALQGITAAAAPYAKITEVHQDEVWELPAGAEVLASSSKTGV 201
           +    AMA    P +               +  I E HQDEVWELP  AEVLA S  TGV
Sbjct: 154 IHPTAAMARLFSPIKL------------PVHMPIIEFHQDEVWELPPQAEVLARSDMTGV 201

Query: 202 EMFCAGDRVLGIQGHPEYTADILLNLVDRLSSAGSITMAVAEGVRRQLEDTGPDREFWIK 261
           EMF  GDR +G+QGHPEY+ DIL+++ DRL     I     +  +   +   PD++ W K
Sbjct: 202 EMFRLGDRAMGVQGHPEYSKDILMSIADRLLRNDLILDHQVDKAKASFDLRQPDKDLWKK 261

Query: 262 LCKSFLK 268
           +C+ FLK
Sbjct: 262 VCRGFLK 268



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.141    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,928,220
Number of extensions: 372102
Number of successful extensions: 833
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 828
Number of HSP's successfully gapped: 3
Length of query: 272
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 173
Effective length of database: 11,866,615
Effective search space: 2052924395
Effective search space used: 2052924395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 155 (64.3 bits)