BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0279400 Os09g0279400|AK067071
         (239 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0279400  Rhodanese-like domain containing protein            437   e-123
Os02g0596000  Rhodanese-like domain containing protein             84   9e-17
Os03g0289400  Similar to Rhodanese-like family protein             79   3e-15

>Os09g0279400 Rhodanese-like domain containing protein
          Length = 239

 Score =  437 bits (1123), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 219/239 (91%), Positives = 219/239 (91%)

Query: 1   MAVLGLSTAFSPPRGSCIAVRIRHXXXXXXXXXXXXXXXXXXXXIGVRAEVSFVDGDEAK 60
           MAVLGLSTAFSPPRGSCIAVRIRH                    IGVRAEVSFVDGDEAK
Sbjct: 1   MAVLGLSTAFSPPRGSCIAVRIRHGARSARSNLSLRRRSAGGGAIGVRAEVSFVDGDEAK 60

Query: 61  RLVAEEGYTVLDIRDRTQRERAHIKNSAHVPLFVENDDGDIGTIIKRTVHSNFAGLFFGL 120
           RLVAEEGYTVLDIRDRTQRERAHIKNSAHVPLFVENDDGDIGTIIKRTVHSNFAGLFFGL
Sbjct: 61  RLVAEEGYTVLDIRDRTQRERAHIKNSAHVPLFVENDDGDIGTIIKRTVHSNFAGLFFGL 120

Query: 121 PFTKRNLEFTKMVKDKFSPESKLLVVCQEGLRSTGAADVLEREGFQNLACIKSGLQTLKP 180
           PFTKRNLEFTKMVKDKFSPESKLLVVCQEGLRSTGAADVLEREGFQNLACIKSGLQTLKP
Sbjct: 121 PFTKRNLEFTKMVKDKFSPESKLLVVCQEGLRSTGAADVLEREGFQNLACIKSGLQTLKP 180

Query: 181 GTFESVGKSELQNAGKAGLVTVQGKISAVLGTVLISAYLFITLFPDQAEKLFDLAGIKL 239
           GTFESVGKSELQNAGKAGLVTVQGKISAVLGTVLISAYLFITLFPDQAEKLFDLAGIKL
Sbjct: 181 GTFESVGKSELQNAGKAGLVTVQGKISAVLGTVLISAYLFITLFPDQAEKLFDLAGIKL 239



>Os02g0596000 Rhodanese-like domain containing protein
          Length = 292

 Score = 84.0 bits (206), Expect = 9e-17,   Method: Compositional matrix adjust.
 Identities = 47/142 (33%), Positives = 75/142 (52%), Gaps = 7/142 (4%)

Query: 65  EEGYT-------VLDIRDRTQRERAHIKNSAHVPLFVENDDGDIGTIIKRTVHSNFAGLF 117
           E GYT       +LD+R   +R++A +K S  +P+F  +   D+G   K+  +    G +
Sbjct: 95  EAGYTFKLTDKVLLDVRPSNERQKAWVKGSTWIPVFDVDTSFDLGGAGKKFTNYVMGGWW 154

Query: 118 FGLPFTKRNLEFTKMVKDKFSPESKLLVVCQEGLRSTGAADVLEREGFQNLACIKSGLQT 177
            G      N  F + V++KFS ++ ++VVCQ+GLRS  A + L   GFQNL  ++ GL+ 
Sbjct: 155 SGSSTMTVNKNFVQQVEEKFSKDTDIIVVCQKGLRSLAACEQLYGAGFQNLFWVQGGLEA 214

Query: 178 LKPGTFESVGKSELQNAGKAGL 199
            +   FE  G    + AG  G+
Sbjct: 215 AEEEDFEREGPQPFKLAGIGGV 236



>Os03g0289400 Similar to Rhodanese-like family protein
          Length = 148

 Score = 79.0 bits (193), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 41/97 (42%), Positives = 57/97 (58%), Gaps = 2/97 (2%)

Query: 58  EAKRLVAEEGYTVLDIRDRTQRERAHIKNSAHVPLFVENDDGDIGTIIKRTVHSNFAGLF 117
           EA   ++ EG+ +LD+R   +R RA ++ SAH PLFV +DD    T++K+ VH  + GL+
Sbjct: 52  EAAGAMSAEGFRLLDVRPEWERARAAVRGSAHAPLFVGDDDTGPVTLLKKWVHFGYIGLW 111

Query: 118 FGLPFTKRNLEFTKMVKDKF--SPESKLLVVCQEGLR 152
            G  FTK N  F   V        ++KLLV C EGLR
Sbjct: 112 TGQSFTKMNDRFLDDVAAAAGEGKDAKLLVACGEGLR 148



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.138    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,711,108
Number of extensions: 236512
Number of successful extensions: 500
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 499
Number of HSP's successfully gapped: 3
Length of query: 239
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 141
Effective length of database: 11,918,829
Effective search space: 1680554889
Effective search space used: 1680554889
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 154 (63.9 bits)