BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0566900 Os08g0566900|AK100851
         (187 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0566900  Mpv17/PMP22 family protein                          360   e-100
Os02g0226000  Similar to Peroxisomal membrane protein PMP22 ...   238   2e-63
AK060354                                                           74   6e-14
Os01g0228300  Mpv17/PMP22 family protein                           73   9e-14

>Os08g0566900 Mpv17/PMP22 family protein
          Length = 187

 Score =  360 bits (925), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 175/187 (93%), Positives = 175/187 (93%)

Query: 1   MSDVVAMAGQAYMRQLQAHPLRTKAITSGVLAGCSDAIAQKISGVPNLQRRRLLLIMLYG 60
           MSDVVAMAGQAYMRQLQAHPLRTKAITSGVLAGCSDAIAQKISGVPNLQRRRLLLIMLYG
Sbjct: 1   MSDVVAMAGQAYMRQLQAHPLRTKAITSGVLAGCSDAIAQKISGVPNLQRRRLLLIMLYG 60

Query: 61  FAYAGPFGHFLHKLMDRFFXXXXXXXXXXXXVLVEQLTASPWNNMMFMMYYGLVVEGRPF 120
           FAYAGPFGHFLHKLMDRFF            VLVEQLTASPWNNMMFMMYYGLVVEGRPF
Sbjct: 61  FAYAGPFGHFLHKLMDRFFKGKKGKETTAKKVLVEQLTASPWNNMMFMMYYGLVVEGRPF 120

Query: 121 SQVKSKLKKDYASVQLTAWKFWPIVSWINYEYMPLQLRVLFHSFVASCWAVFLNLKAARS 180
           SQVKSKLKKDYASVQLTAWKFWPIVSWINYEYMPLQLRVLFHSFVASCWAVFLNLKAARS
Sbjct: 121 SQVKSKLKKDYASVQLTAWKFWPIVSWINYEYMPLQLRVLFHSFVASCWAVFLNLKAARS 180

Query: 181 IATSKKA 187
           IATSKKA
Sbjct: 181 IATSKKA 187



>Os02g0226000 Similar to Peroxisomal membrane protein PMP22 (22 kDa peroxisomal
           membrane protein)
          Length = 205

 Score =  238 bits (607), Expect = 2e-63,   Method: Compositional matrix adjust.
 Identities = 106/170 (62%), Positives = 133/170 (78%)

Query: 8   AGQAYMRQLQAHPLRTKAITSGVLAGCSDAIAQKISGVPNLQRRRLLLIMLYGFAYAGPF 67
           A + Y+RQLQ HPLRTK IT+G LAG SD++AQK+SG   +++RRLLL ML+GFAY GPF
Sbjct: 28  AWRQYLRQLQLHPLRTKMITAGCLAGVSDSVAQKLSGYQRIEKRRLLLKMLFGFAYGGPF 87

Query: 68  GHFLHKLMDRFFXXXXXXXXXXXXVLVEQLTASPWNNMMFMMYYGLVVEGRPFSQVKSKL 127
           GHFLHK++D  F            VL+EQ+T+SPWNN++F+ YYG VVE RPF +VK+++
Sbjct: 88  GHFLHKVLDYIFKGKKDTKTIAKKVLLEQITSSPWNNLLFLFYYGYVVERRPFKEVKTRV 147

Query: 128 KKDYASVQLTAWKFWPIVSWINYEYMPLQLRVLFHSFVASCWAVFLNLKA 177
           KK Y SVQL+AW FWPIV WIN+ YMPLQ RV+FHSFVA CW +FLNL+A
Sbjct: 148 KKQYPSVQLSAWMFWPIVGWINHMYMPLQFRVIFHSFVACCWGIFLNLRA 197



>AK060354 
          Length = 262

 Score = 73.9 bits (180), Expect = 6e-14,   Method: Compositional matrix adjust.
 Identities = 50/171 (29%), Positives = 77/171 (45%), Gaps = 19/171 (11%)

Query: 11  AYMRQLQAHPLRTKAITSGVLAGCSDA----IAQKISGVPNLQRRRLLLIMLYGFAYAGP 66
           AY++QLQ++PLRTK +TSG L+G  +     IA   S   +    R+  + LYG   + P
Sbjct: 48  AYLKQLQSNPLRTKILTSGTLSGLQEFLASWIAHDRSKSGHYFTSRVPKMALYGALISAP 107

Query: 67  FGHFLHKLMDRFFXXXXXXXXXXXXVLVEQLTASPWNNMMFMMYYGLVVEGRPFSQVKSK 126
            GH L  ++ + F            +L   L  SP  N ++++   ++   R F QV++ 
Sbjct: 108 LGHVLISILQKVFQGRKSLKAKILQILASNLIISPIQNSVYLVSMAIIAGARTFHQVRAT 167

Query: 127 LKKDYASVQLTAWKFWPI--VSWINYEYMPLQLRVLFHSFVASCWAVFLNL 175
           +K            FWP+  VSWI     P+ L         S W  F N+
Sbjct: 168 VKAG----------FWPVMKVSWI---VSPISLAFAQQFLPESTWVPFFNI 205



>Os01g0228300 Mpv17/PMP22 family protein
          Length = 222

 Score = 73.2 bits (178), Expect = 9e-14,   Method: Compositional matrix adjust.
 Identities = 46/176 (26%), Positives = 80/176 (45%), Gaps = 27/176 (15%)

Query: 12  YMRQLQAHPLRTKAITSGVLAGCSDAIAQKIS-----GVP------------------NL 48
           Y + L  HP+RT+ ++SG+L G  D  AQ ++     G P                   +
Sbjct: 8   YQQCLATHPVRTQVVSSGILWGLGDIGAQAVTHYSAPGRPRHHQHHAKNPPEDKDKEFKI 67

Query: 49  QRRRLLLIMLYGFAYAGPFGHFLHKLMDRFFXXXXXXXX---XXXXVLVEQLTASPWNNM 105
             +R+ +   +GFA+ GP GH+ ++ +DRF                V  + L   P + +
Sbjct: 68  DWKRVGITSSFGFAFVGPVGHYWYEYLDRFILRRYQPKTFKFVASKVAADGLLFGPVDLL 127

Query: 106 MFMMYYGLVVEGRPFSQVKSKLKKDYASVQLTAWKFWPIVSWINYEYMPLQLRVLF 161
           +F  Y GL   GR   QVK  +K+D+    +     WP V   N+ ++P++ ++L+
Sbjct: 128 LFFSYVGLA-SGRSVEQVKDDVKRDFIPALVLGGTIWPAVQIANFRFIPVRYQLLY 182



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.136    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,228,230
Number of extensions: 176151
Number of successful extensions: 376
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 374
Number of HSP's successfully gapped: 5
Length of query: 187
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 92
Effective length of database: 12,075,471
Effective search space: 1110943332
Effective search space used: 1110943332
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 153 (63.5 bits)