BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0562600 Os08g0562600|AK064971
         (291 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0562600  C2 calcium/lipid-binding region, CaLB domain c...   315   2e-86
Os09g0571200  C2 domain containing protein                        266   1e-71
Os01g0853800  C2 calcium/lipid-binding region, CaLB domain c...   112   3e-25

>Os08g0562600 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 291

 Score =  315 bits (808), Expect = 2e-86,   Method: Compositional matrix adjust.
 Identities = 158/158 (100%), Positives = 158/158 (100%)

Query: 1   MGSRYEVEVTVSSARDLKNVNWRNGDLKPYAVLWVDDGAKCSTRVDLDNADNPNWDDKLT 60
           MGSRYEVEVTVSSARDLKNVNWRNGDLKPYAVLWVDDGAKCSTRVDLDNADNPNWDDKLT
Sbjct: 1   MGSRYEVEVTVSSARDLKNVNWRNGDLKPYAVLWVDDGAKCSTRVDLDNADNPNWDDKLT 60

Query: 61  LPLPPSSRLDDALLYLDVVHANAAEGVKPLVGSARLPLRDVLDDAGVGARVSRSLRLKRP 120
           LPLPPSSRLDDALLYLDVVHANAAEGVKPLVGSARLPLRDVLDDAGVGARVSRSLRLKRP
Sbjct: 61  LPLPPSSRLDDALLYLDVVHANAAEGVKPLVGSARLPLRDVLDDAGVGARVSRSLRLKRP 120

Query: 121 SGRPQGRLDLRLAVRESARYYDPSPYPAPYGQPAGTRD 158
           SGRPQGRLDLRLAVRESARYYDPSPYPAPYGQPAGTRD
Sbjct: 121 SGRPQGRLDLRLAVRESARYYDPSPYPAPYGQPAGTRD 158



>Os09g0571200 C2 domain containing protein
          Length = 290

 Score =  266 bits (680), Expect = 1e-71,   Method: Compositional matrix adjust.
 Identities = 130/152 (85%), Positives = 141/152 (92%), Gaps = 1/152 (0%)

Query: 2   GSRYEVEVTVSSARDLKNVNWRNGDLKPYAVLWVDDGAKCSTRVDLDNADNPNWDDKLTL 61
            SRYEVEVTV+SARDLKNVNWRNGDLKPYAV+W+DDGAKCSTRVDLDNADNP WDDKLT+
Sbjct: 3   ASRYEVEVTVASARDLKNVNWRNGDLKPYAVVWIDDGAKCSTRVDLDNADNPTWDDKLTV 62

Query: 62  PLPPSSRLDDALLYLDVVHANAAEGVKPLVGSARLPLRDVLDDAGVGARVSRSLRLKRPS 121
           PLPPS+RLDDA+LYLDVVHANA +GVKPLVGSARLPLRDVL D G+GAR SRSLRLKRPS
Sbjct: 63  PLPPSTRLDDAVLYLDVVHANATDGVKPLVGSARLPLRDVLADTGIGARASRSLRLKRPS 122

Query: 122 GRPQGRLDLRLAVRESARYYDPSP-YPAPYGQ 152
           GRP GRL++R+AVRE  RYYDPSP YPAPY Q
Sbjct: 123 GRPHGRLEVRVAVREPKRYYDPSPAYPAPYHQ 154



>Os01g0853800 C2 calcium/lipid-binding region, CaLB domain containing protein
          Length = 327

 Score =  112 bits (281), Expect = 3e-25,   Method: Compositional matrix adjust.
 Identities = 66/141 (46%), Positives = 88/141 (62%), Gaps = 12/141 (8%)

Query: 6   EVEVTVSSARDLKNVNWRNGDLKPYAVLWVDDGAKCSTRVDLDNADNPNWDDKLTLPLPP 65
           ++EVTV S + LKNVNWR GDL+ Y V ++D   + +TR D      P W++++ LPLPP
Sbjct: 49  DLEVTVVSGKHLKNVNWRRGDLRAYVVAYLDPSRRAATRPDDVGGCKPAWNERVVLPLPP 108

Query: 66  SSRLDDA--LLYLDVVHANAAEGVKPLVGSARLPLRDVL--------DDAGVGARVSRSL 115
                D   LL LDV H+  ++  KPLVGSAR PLRD+L         D+   A +  +L
Sbjct: 109 HLSPHDPSLLLSLDVFHSKPSDSPKPLVGSARSPLRDLLFSTNPNPSPDSPASALI--TL 166

Query: 116 RLKRPSGRPQGRLDLRLAVRE 136
            L RPSGRPQG+L +RLA+RE
Sbjct: 167 PLLRPSGRPQGKLRIRLALRE 187



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,832,801
Number of extensions: 296540
Number of successful extensions: 608
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 606
Number of HSP's successfully gapped: 3
Length of query: 291
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 191
Effective length of database: 11,814,401
Effective search space: 2256550591
Effective search space used: 2256550591
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 155 (64.3 bits)