BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0485900 Os08g0485900|AK110716
         (324 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0485900  Haloacid dehalogenase-like hydrolase domain co...   527   e-150
Os03g0565200  Haloacid dehalogenase-like hydrolase domain co...   167   8e-42
Os10g0464400  Haloacid dehalogenase/epoxide hydrolase family...    69   6e-12

>Os08g0485900 Haloacid dehalogenase-like hydrolase domain containing protein
          Length = 324

 Score =  527 bits (1358), Expect = e-150,   Method: Compositional matrix adjust.
 Identities = 259/295 (87%), Positives = 259/295 (87%)

Query: 30  VLSPPRRLATLRCLGYXXXXXXXXXXXXXXXXXXXXXXXXLIFDCDGVILESEHLHRQAY 89
           VLSPPRRLATLRCLGY                        LIFDCDGVILESEHLHRQAY
Sbjct: 30  VLSPPRRLATLRCLGYARRRAQARHVVACASAASAGSLDALIFDCDGVILESEHLHRQAY 89

Query: 90  NDAFAHFGVSCEPASAAATDAPLYWDEAFYDDLQNRIGGGKPKMRWYFGENGWPTSKIFE 149
           NDAFAHFGVSCEPASAAATDAPLYWDEAFYDDLQNRIGGGKPKMRWYFGENGWPTSKIFE
Sbjct: 90  NDAFAHFGVSCEPASAAATDAPLYWDEAFYDDLQNRIGGGKPKMRWYFGENGWPTSKIFE 149

Query: 150 TPPSSDSDKEKLVDIIQDWKTERYKEILKSGTVKPRPGVLRLMDEVKGAGIKLAVCSAAT 209
           TPPSSDSDKEKLVDIIQDWKTERYKEILKSGTVKPRPGVLRLMDEVKGAGIKLAVCSAAT
Sbjct: 150 TPPSSDSDKEKLVDIIQDWKTERYKEILKSGTVKPRPGVLRLMDEVKGAGIKLAVCSAAT 209

Query: 210 KSSVIMCLENLIGLERFNGLDCFLAGDDVKLKKPDPSIYITAAEKLGVQSQNCLVVEDSV 269
           KSSVIMCLENLIGLERFNGLDCFLAGDDVKLKKPDPSIYITAAEKLGVQSQNCLVVEDSV
Sbjct: 210 KSSVIMCLENLIGLERFNGLDCFLAGDDVKLKKPDPSIYITAAEKLGVQSQNCLVVEDSV 269

Query: 270 IGLQAAKGAGMSCIITYTPSTANQDFSDAIATYPDLSNVGXXXXXXXXXXXXVTG 324
           IGLQAAKGAGMSCIITYTPSTANQDFSDAIATYPDLSNVG            VTG
Sbjct: 270 IGLQAAKGAGMSCIITYTPSTANQDFSDAIATYPDLSNVGLEDLKLLLQKSLVTG 324



>Os03g0565200 Haloacid dehalogenase-like hydrolase domain containing protein
          Length = 320

 Score =  167 bits (424), Expect = 8e-42,   Method: Compositional matrix adjust.
 Identities = 95/234 (40%), Positives = 137/234 (58%), Gaps = 24/234 (10%)

Query: 72  FDCDGVILESEH-LHRQAYNDAFAH--FGVSCEPASAAATDAPLYWDEAFYDDLQNRIGG 128
           FDCDGV++++E   HR ++N+ FA    GVS              WD   Y +L  +IGG
Sbjct: 82  FDCDGVLVDTEKDGHRISFNETFAERELGVS--------------WDVELYGELL-KIGG 126

Query: 129 GKPKMRWYFGENGWPTSKIFETPPSSDSDKEKLVDIIQDWKTERYKEILKSGTVKPRPGV 188
           GK +M  YF + GWP        P +D ++++ +  +   KTE +  +++   +  RPGV
Sbjct: 127 GKERMTAYFSKMGWPAKA-----PKTDDERKEFIASLHKRKTELFMALIEKKLLPLRPGV 181

Query: 189 LRLMDEVKGAGIKLAVCSAATKSSVIMCLENLIGLERFNGLDCFLAGDDVKLKKPDPSIY 248
            RL+DE  G G+K+AVCS + + +V   +  L+G +R   +  F AGD V  KKPDP+IY
Sbjct: 182 QRLIDEALGKGVKVAVCSTSNEKAVSAIVSCLLGPDRAEKITIF-AGDVVPRKKPDPAIY 240

Query: 249 ITAAEKLGVQSQNCLVVEDSVIGLQAAKGAGMSCIITYTPSTANQDFSDAIATY 302
           + AA  LGV   +C+VVEDS IGL AAK AGM CI+T +  TA +DF+ A A +
Sbjct: 241 LLAATTLGVDPSSCVVVEDSTIGLAAAKAAGMKCIVTKSGYTAEEDFATADAVF 294



>Os10g0464400 Haloacid dehalogenase/epoxide hydrolase family protein
          Length = 397

 Score = 68.6 bits (166), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 40/121 (33%), Positives = 65/121 (53%), Gaps = 8/121 (6%)

Query: 181 TVKPRPGVLRLMDEVKGAGIKLAVCSAATKSSV---IMCLENLIGLERFNGLDCFLAGDD 237
            +K  PG  RL+  +K  G+  A+ S + +S++   I C +     E F+ +   + GD+
Sbjct: 91  NIKALPGANRLIKHLKSNGVPAALASNSPRSNIDAKISCHQGW--KESFSAI---VGGDE 145

Query: 238 VKLKKPDPSIYITAAEKLGVQSQNCLVVEDSVIGLQAAKGAGMSCIITYTPSTANQDFSD 297
           V+  KP P I++ AA+++     NCLV+EDS+ G+ A K AGM  I   +      +FS 
Sbjct: 146 VEKGKPSPDIFLEAAKRMNTNPPNCLVIEDSLPGVAAGKAAGMHVIAVPSVPKRTAEFSS 205

Query: 298 A 298
           A
Sbjct: 206 A 206



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.136    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,943,607
Number of extensions: 419585
Number of successful extensions: 1024
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1022
Number of HSP's successfully gapped: 3
Length of query: 324
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 223
Effective length of database: 11,762,187
Effective search space: 2622967701
Effective search space used: 2622967701
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)