BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0320400 Os08g0320400|J065059G01
(103 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os08g0320400 Indole-3-glycerol phosphate synthase domain co... 206 3e-54
Os09g0255400 Similar to Indole-3-glycerol phosphate synthas... 174 2e-44
Os04g0467700 Similar to Indole-3-glycerol phosphate synthas... 153 3e-38
>Os08g0320400 Indole-3-glycerol phosphate synthase domain containing protein
Length = 103
Score = 206 bits (524), Expect = 3e-54, Method: Compositional matrix adjust.
Identities = 103/103 (100%), Positives = 103/103 (100%)
Query: 1 VHDEREMERVLNISGVQLIGINNRSLETFVVDTSNTKMLLDMHGDTIREKGILVVGESGL 60
VHDEREMERVLNISGVQLIGINNRSLETFVVDTSNTKMLLDMHGDTIREKGILVVGESGL
Sbjct: 1 VHDEREMERVLNISGVQLIGINNRSLETFVVDTSNTKMLLDMHGDTIREKGILVVGESGL 60
Query: 61 FNPDDVAYVQNAGVSAVLVGESLVKQDDPGRAIAGLFGKELLH 103
FNPDDVAYVQNAGVSAVLVGESLVKQDDPGRAIAGLFGKELLH
Sbjct: 61 FNPDDVAYVQNAGVSAVLVGESLVKQDDPGRAIAGLFGKELLH 103
>Os09g0255400 Similar to Indole-3-glycerol phosphate synthase, chloroplast
precursor (EC 4.1.1.48) (IGPS)
Length = 381
Score = 174 bits (440), Expect = 2e-44, Method: Composition-based stats.
Identities = 85/103 (82%), Positives = 95/103 (92%)
Query: 1 VHDEREMERVLNISGVQLIGINNRSLETFVVDTSNTKMLLDMHGDTIREKGILVVGESGL 60
VHDERE++RVL I GVQLIGINNRSLETF VDTSNTK LL+ GD IR+KGILVVGESGL
Sbjct: 279 VHDERELDRVLKIDGVQLIGINNRSLETFKVDTSNTKTLLEKRGDLIRDKGILVVGESGL 338
Query: 61 FNPDDVAYVQNAGVSAVLVGESLVKQDDPGRAIAGLFGKELLH 103
F PDDVAYVQNAGVSA+LVGESLVKQ++PG+AIAGL+GK+LLH
Sbjct: 339 FTPDDVAYVQNAGVSAILVGESLVKQENPGQAIAGLYGKDLLH 381
>Os04g0467700 Similar to Indole-3-glycerol phosphate synthase, chloroplast
precursor (EC 4.1.1.48) (IGPS)
Length = 246
Score = 153 bits (386), Expect = 3e-38, Method: Compositional matrix adjust.
Identities = 76/102 (74%), Positives = 89/102 (87%), Gaps = 1/102 (0%)
Query: 1 VHDEREMERVLNISGVQLIGINNRSLETFVVDTSNTKMLLD-MHGDTIREKGILVVGESG 59
VHDEREM+RVL I GVQLIGINNR+LETF VD SNTK LL+ G TI +KGI+VVGESG
Sbjct: 139 VHDEREMDRVLGIDGVQLIGINNRNLETFQVDISNTKNLLEGERGQTIAQKGIIVVGESG 198
Query: 60 LFNPDDVAYVQNAGVSAVLVGESLVKQDDPGRAIAGLFGKEL 101
LF PD +++VQNAGV AVLVGESL+KQ+DPG+AIAGLFGK++
Sbjct: 199 LFTPDHISFVQNAGVKAVLVGESLIKQEDPGKAIAGLFGKDI 240
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.317 0.138 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,217,891
Number of extensions: 115015
Number of successful extensions: 381
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 380
Number of HSP's successfully gapped: 3
Length of query: 103
Length of database: 17,035,801
Length adjustment: 71
Effective length of query: 32
Effective length of database: 13,328,607
Effective search space: 426515424
Effective search space used: 426515424
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 149 (62.0 bits)