BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0269700 Os08g0269700|AK063209
(136 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os08g0269700 Conserved hypothetical protein 155 8e-39
Os08g0269500 Conserved hypothetical protein 92 8e-20
Os08g0269600 EGF-like region domain containing protein 69 1e-12
>Os08g0269700 Conserved hypothetical protein
Length = 136
Score = 155 bits (392), Expect = 8e-39, Method: Compositional matrix adjust.
Identities = 78/107 (72%), Positives = 78/107 (72%)
Query: 1 MKKXXXXXXXXXXXXXXQGTWCAAERSHRINGAHLRPHVQVEESHITEGKKLLEIQVPRK 60
MKK QGTWCAAERSHRINGAHLRPHVQVEESHITEGKKLLEIQVPRK
Sbjct: 1 MKKSSLAVFLLLLTLASQGTWCAAERSHRINGAHLRPHVQVEESHITEGKKLLEIQVPRK 60
Query: 61 LGHEVKFPKRMAIAHKXXXXXXXXXXXXXXXASNVNSGPADTRPRKN 107
LGHEVKFPKRMAIAHK ASNVNSGPADTRPRKN
Sbjct: 61 LGHEVKFPKRMAIAHKGGSTGGGSTGGTTGGASNVNSGPADTRPRKN 107
>Os08g0269500 Conserved hypothetical protein
Length = 131
Score = 92.4 bits (228), Expect = 8e-20, Method: Compositional matrix adjust.
Identities = 47/60 (78%), Positives = 50/60 (83%), Gaps = 5/60 (8%)
Query: 19 GTWCAA--ERSHRINGAHLRPHVQVEESHITEGKKLLEIQVPRKLGHEVKFPKRMAIAHK 76
GTWCAA +RSHRI AHLRPH+ VEE H GKKL+EIQVPRKLGHEVK PKRMAIAHK
Sbjct: 19 GTWCAAAADRSHRITTAHLRPHLHVEELH---GKKLMEIQVPRKLGHEVKVPKRMAIAHK 75
>Os08g0269600 EGF-like region domain containing protein
Length = 143
Score = 68.6 bits (166), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 38/67 (56%), Positives = 42/67 (62%), Gaps = 8/67 (11%)
Query: 18 QGTWCAAERSHRINGAHLRPHVQVEESHITEGKKLLEIQV--------PRKLGHEVKFPK 69
Q TWC AERSH INGA LRPH+Q EE H+T+GKKL + K EVK P
Sbjct: 19 QATWCDAERSHAINGARLRPHLQFEELHVTDGKKLGQAADTDTDTDTESHKHHDEVKVPM 78
Query: 70 RMAIAHK 76
RMAIAHK
Sbjct: 79 RMAIAHK 85
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.315 0.129 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,646,283
Number of extensions: 75038
Number of successful extensions: 96
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 94
Number of HSP's successfully gapped: 3
Length of query: 136
Length of database: 17,035,801
Length adjustment: 90
Effective length of query: 46
Effective length of database: 12,336,541
Effective search space: 567480886
Effective search space used: 567480886
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 150 (62.4 bits)