BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0162000 Os08g0162000|AK119463
         (68 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0162000  Nonaspanin (TM9SF) family protein                    91   3e-19
Os03g0237000  Nonaspanin (TM9SF) family protein                    83   4e-17
Os10g0112600  Nonaspanin (TM9SF) family protein                    79   1e-15

>Os08g0162000 Nonaspanin (TM9SF) family protein
          Length = 68

 Score = 90.5 bits (223), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 42/42 (100%), Positives = 42/42 (100%)

Query: 27 DGSDHRYKDGDYVPLYANKVGPFHNPSETYRYYDLPFCAPGL 68
          DGSDHRYKDGDYVPLYANKVGPFHNPSETYRYYDLPFCAPGL
Sbjct: 27 DGSDHRYKDGDYVPLYANKVGPFHNPSETYRYYDLPFCAPGL 68



>Os03g0237000 Nonaspanin (TM9SF) family protein
          Length = 595

 Score = 83.2 bits (204), Expect = 4e-17,   Method: Composition-based stats.
 Identities = 34/40 (85%), Positives = 37/40 (92%)

Query: 27 DGSDHRYKDGDYVPLYANKVGPFHNPSETYRYYDLPFCAP 66
          DGSDHRYK  + VPLYANKVGPFHNPSETYRY+DLPFC+P
Sbjct: 28 DGSDHRYKANELVPLYANKVGPFHNPSETYRYFDLPFCSP 67



>Os10g0112600 Nonaspanin (TM9SF) family protein
          Length = 585

 Score = 78.6 bits (192), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 32/36 (88%), Positives = 34/36 (94%)

Query: 31 HRYKDGDYVPLYANKVGPFHNPSETYRYYDLPFCAP 66
          HRY+ GD VPLYANKVGPFHNPSETYRY+DLPFCAP
Sbjct: 25 HRYRPGDAVPLYANKVGPFHNPSETYRYFDLPFCAP 60



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.146    0.490 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,927,062
Number of extensions: 55538
Number of successful extensions: 71
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 71
Number of HSP's successfully gapped: 3
Length of query: 68
Length of database: 17,035,801
Length adjustment: 40
Effective length of query: 28
Effective length of database: 14,947,241
Effective search space: 418522748
Effective search space used: 418522748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)