BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0102800 Os08g0102800|AK120363
(156 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os08g0102800 Conserved hypothetical protein 304 2e-83
Os07g0189700 Conserved hypothetical protein 107 3e-24
Os07g0189800 Conserved hypothetical protein 103 5e-23
Os07g0475000 Conserved hypothetical protein 84 6e-17
Os03g0850800 Conserved hypothetical protein 71 3e-13
>Os08g0102800 Conserved hypothetical protein
Length = 156
Score = 304 bits (778), Expect = 2e-83, Method: Compositional matrix adjust.
Identities = 149/156 (95%), Positives = 149/156 (95%)
Query: 1 MAPTLPSWWXXXXXXXGVGATAAARGSKKLTPPVSTAYEWPERFHAVVVSINLTNHDRGG 60
MAPTLPSWW GVGATAAARGSKKLTPPVSTAYEWPERFHAVVVSINLTNHDRGG
Sbjct: 1 MAPTLPSWWLLFLLLLGVGATAAARGSKKLTPPVSTAYEWPERFHAVVVSINLTNHDRGG 60
Query: 61 GRLQLIEIYYDWPHGRDLNIVRDQLSGDPPLYNVEWVNGTSYLFDTAASSCRTFQFPVGI 120
GRLQLIEIYYDWPHGRDLNIVRDQLSGDPPLYNVEWVNGTSYLFDTAASSCRTFQFPVGI
Sbjct: 61 GRLQLIEIYYDWPHGRDLNIVRDQLSGDPPLYNVEWVNGTSYLFDTAASSCRTFQFPVGI 120
Query: 121 LPRSGVTPWTPTSPLAAPSAGYSTAIRGMCSCSKPV 156
LPRSGVTPWTPTSPLAAPSAGYSTAIRGMCSCSKPV
Sbjct: 121 LPRSGVTPWTPTSPLAAPSAGYSTAIRGMCSCSKPV 156
>Os07g0189700 Conserved hypothetical protein
Length = 225
Score = 107 bits (267), Expect = 3e-24, Method: Compositional matrix adjust.
Identities = 54/83 (65%), Positives = 64/83 (77%), Gaps = 7/83 (8%)
Query: 40 WPERFHAVVVSINLTNHDRGGGRLQLIEIYYDWPHGRDLNIVRDQLSGDPPLYNVEWVNG 99
WPE+FHAVV TN GGRLQLI++YYDWP GR+LN++R QLSGDP Y+VEW NG
Sbjct: 27 WPEQFHAVV----FTNLTGSGGRLQLIDLYYDWPRGRNLNLIRGQLSGDPT-YDVEWTNG 81
Query: 100 TSYLFDTAASSCRTFQFPVGILP 122
TSY F+ A+SCRT FPVG+LP
Sbjct: 82 TSYFFN--ATSCRTKLFPVGLLP 102
>Os07g0189800 Conserved hypothetical protein
Length = 226
Score = 103 bits (257), Expect = 5e-23, Method: Compositional matrix adjust.
Identities = 53/81 (65%), Positives = 64/81 (79%), Gaps = 5/81 (6%)
Query: 42 ERFHAVVVSINLTNHDRGGGRLQLIEIYYDWPHGRDLNIVRDQLSGDPPLYNVEWVNGTS 101
ERFHAV+ + NLT GGG+LQ+ ++YYDWP GR+LN+V QLSGD P+Y+VEW NGTS
Sbjct: 36 ERFHAVLFT-NLT-QTSGGGKLQMTDLYYDWPGGRNLNLVAGQLSGD-PVYDVEWTNGTS 92
Query: 102 YLFDTAASSCRTFQFPVGILP 122
Y FD+A SCRT FPVGILP
Sbjct: 93 YYFDSA--SCRTLLFPVGILP 111
>Os07g0475000 Conserved hypothetical protein
Length = 246
Score = 83.6 bits (205), Expect = 6e-17, Method: Compositional matrix adjust.
Identities = 45/93 (48%), Positives = 62/93 (66%), Gaps = 6/93 (6%)
Query: 32 PPVSTAYEWPERFHAVVVSINLTNHDRG--GGRLQLIEIYYDWPHGRDLNIVRDQLSG-D 88
PP WP+ FHA + + NLTNH G L++ ++YYDW R+LN++R QL+ D
Sbjct: 26 PPEPKPARWPDVFHARLFT-NLTNHSAASTGPPLRVADLYYDWSRRRNLNLIRYQLAAAD 84
Query: 89 PPLYNVEWVNGTSYLFDTAASSCRTFQFPVGIL 121
PLY+VEW NGT++ FD+ SCRT +FPVG+L
Sbjct: 85 DPLYDVEWDNGTTFYFDS--RSCRTERFPVGVL 115
>Os03g0850800 Conserved hypothetical protein
Length = 214
Score = 71.2 bits (173), Expect = 3e-13, Method: Compositional matrix adjust.
Identities = 31/68 (45%), Positives = 46/68 (67%)
Query: 61 GRLQLIEIYYDWPHGRDLNIVRDQLSGDPPLYNVEWVNGTSYLFDTAASSCRTFQFPVGI 120
G L L +++YDWP GR+L+++R QL+ D P Y+ EW NGTS+ + A +CR+ VGI
Sbjct: 46 GNLSLADLWYDWPGGRNLHVIRYQLAADEPFYDNEWNNGTSFFYTPARRACRSAAVGVGI 105
Query: 121 LPRSGVTP 128
LP + + P
Sbjct: 106 LPPNWLVP 113
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.318 0.135 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,405,390
Number of extensions: 279590
Number of successful extensions: 659
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 649
Number of HSP's successfully gapped: 5
Length of query: 156
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 64
Effective length of database: 12,232,113
Effective search space: 782855232
Effective search space used: 782855232
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 151 (62.8 bits)