BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os07g0671400 Os07g0671400|AK067933
(296 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os07g0671400 Heavy metal transport/detoxification protein d... 115 5e-26
Os05g0150700 Heavy metal transport/detoxification protein d... 71 1e-12
Os01g0933200 Heavy metal transport/detoxification protein d... 68 6e-12
AK063326 68 6e-12
>Os07g0671400 Heavy metal transport/detoxification protein domain containing
protein
Length = 296
Score = 115 bits (287), Expect = 5e-26, Method: Compositional matrix adjust.
Identities = 54/54 (100%), Positives = 54/54 (100%)
Query: 1 MASGEAEPLQYTTTVLRVSIHCEGCKKKVKKVLQNIEGVYKVTIDAAQHKPDEV 54
MASGEAEPLQYTTTVLRVSIHCEGCKKKVKKVLQNIEGVYKVTIDAAQHKPDEV
Sbjct: 1 MASGEAEPLQYTTTVLRVSIHCEGCKKKVKKVLQNIEGVYKVTIDAAQHKPDEV 54
Score = 108 bits (269), Expect = 6e-24, Method: Compositional matrix adjust.
Identities = 61/106 (57%), Positives = 61/106 (57%)
Query: 191 TLSSPERPHGGFPYYSPQPVMSYNVAHPSASVSSYYAXXXXXXXXXXXXXXXXXXXXXXX 250
TLSSPERPHGGFPYYSPQPVMSYNVAHPSASVSSYYA
Sbjct: 191 TLSSPERPHGGFPYYSPQPVMSYNVAHPSASVSSYYAPTPVMSMQPTPMPPPPQMSYGYS 250
Query: 251 XXXXXXXXXXXXXXXXXXXXXXRSSPPQESYSNMFNEENPSSCSVM 296
RSSPPQESYSNMFNEENPSSCSVM
Sbjct: 251 PYPPMMMPPPPPPEYLYGPPGMRSSPPQESYSNMFNEENPSSCSVM 296
>Os05g0150700 Heavy metal transport/detoxification protein domain containing
protein
Length = 368
Score = 70.9 bits (172), Expect = 1e-12, Method: Compositional matrix adjust.
Identities = 34/52 (65%), Positives = 38/52 (73%), Gaps = 2/52 (3%)
Query: 1 MASGE--AEPLQYTTTVLRVSIHCEGCKKKVKKVLQNIEGVYKVTIDAAQHK 50
MA+ E EPL+Y T L+VSIHCEGCKKKVKKVL +IEGVYK ID K
Sbjct: 1 MAAAEEGPEPLKYQTLALKVSIHCEGCKKKVKKVLHSIEGVYKTDIDVQHQK 52
>Os01g0933200 Heavy metal transport/detoxification protein domain containing
protein
Length = 336
Score = 68.2 bits (165), Expect = 6e-12, Method: Compositional matrix adjust.
Identities = 31/50 (62%), Positives = 40/50 (80%), Gaps = 1/50 (2%)
Query: 1 MASGEAEPLQYTTTVLRVSIHCEGCKKKVKKVLQNIEGVYKVTIDAAQHK 50
MASG +EP++ VLRVSIHCEGCKKKVKKVLQ++ GV++ +DA +K
Sbjct: 1 MASG-SEPIECQVLVLRVSIHCEGCKKKVKKVLQHVPGVFRCDVDARSNK 49
>AK063326
Length = 336
Score = 68.2 bits (165), Expect = 6e-12, Method: Compositional matrix adjust.
Identities = 31/50 (62%), Positives = 40/50 (80%), Gaps = 1/50 (2%)
Query: 1 MASGEAEPLQYTTTVLRVSIHCEGCKKKVKKVLQNIEGVYKVTIDAAQHK 50
MASG +EP++ VLRVSIHCEGCKKKVKKVLQ++ GV++ +DA +K
Sbjct: 1 MASG-SEPIECQVLVLRVSIHCEGCKKKVKKVLQHVPGVFRCDVDARSNK 49
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.308 0.123 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,384,815
Number of extensions: 96934
Number of successful extensions: 253
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 253
Number of HSP's successfully gapped: 5
Length of query: 296
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 196
Effective length of database: 11,814,401
Effective search space: 2315622596
Effective search space used: 2315622596
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 156 (64.7 bits)