BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0619200 Os07g0619200|AK100302
         (103 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0619200  Calcium-binding EF-hand domain containing protein   210   2e-55
Os03g0395200                                                      105   8e-24
Os09g0458800  Calcium-binding EF-hand domain containing protein    83   4e-17
Os09g0458900  Calcium-binding EF-hand domain containing protein    81   1e-16
Os02g0762900  EF-Hand type domain containing protein               75   9e-15
Os06g0213300                                                       72   9e-14

>Os07g0619200 Calcium-binding EF-hand domain containing protein
          Length = 103

 Score =  210 bits (534), Expect = 2e-55,   Method: Compositional matrix adjust.
 Identities = 103/103 (100%), Positives = 103/103 (100%)

Query: 1   AITLVKLLQTPAMAIRGIPSSREMTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWF 60
           AITLVKLLQTPAMAIRGIPSSREMTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWF
Sbjct: 1   AITLVKLLQTPAMAIRGIPSSREMTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWF 60

Query: 61  SGLKAGRAVRHADRDNSGFVDESEIENLVAFAQKTLGMKVTAW 103
           SGLKAGRAVRHADRDNSGFVDESEIENLVAFAQKTLGMKVTAW
Sbjct: 61  SGLKAGRAVRHADRDNSGFVDESEIENLVAFAQKTLGMKVTAW 103



>Os03g0395200 
          Length = 149

 Score =  105 bits (262), Expect = 8e-24,   Method: Compositional matrix adjust.
 Identities = 54/98 (55%), Positives = 70/98 (71%), Gaps = 2/98 (2%)

Query: 8   LQTPAMAIRGIPSSRE-MTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWFSGL-KA 65
           L+ PAM  R I SS   MT+++FK W  +F+T+NDGRIS  ELREAIR +G  FS   K+
Sbjct: 8   LKIPAMRCRAIISSPPPMTLEEFKKWFMKFNTNNDGRISGVELREAIRSKGFGFSAWWKS 67

Query: 66  GRAVRHADRDNSGFVDESEIENLVAFAQKTLGMKVTAW 103
             A+  AD+D +G++DE EIENLV FAQK LG+K+T W
Sbjct: 68  IVALHQADKDRNGYIDEFEIENLVTFAQKVLGIKITTW 105



>Os09g0458800 Calcium-binding EF-hand domain containing protein
          Length = 105

 Score = 83.2 bits (204), Expect = 4e-17,   Method: Compositional matrix adjust.
 Identities = 39/85 (45%), Positives = 56/85 (65%)

Query: 19  PSSREMTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWFSGLKAGRAVRHADRDNSG 78
           P +     + FK W++QFDTD DGRISR ELR A+R  G  F+G+K  R + HAD D  G
Sbjct: 21  PFNETAVREKFKEWIRQFDTDGDGRISRDELRRAMRAVGVRFTGIKCRRGMSHADADGDG 80

Query: 79  FVDESEIENLVAFAQKTLGMKVTAW 103
           ++D+SEI+ L+ +  + LG+ V A+
Sbjct: 81  YIDDSEIDGLIEYWGRRLGLGVAAY 105



>Os09g0458900 Calcium-binding EF-hand domain containing protein
          Length = 98

 Score = 81.3 bits (199), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 37/81 (45%), Positives = 60/81 (74%)

Query: 23  EMTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWFSGLKAGRAVRHADRDNSGFVDE 82
           +MTVD+FK WL++FD D DGRISR ELR A+R     F+G ++ + + +AD D  G++D+
Sbjct: 18  DMTVDEFKEWLRRFDVDRDGRISRDELRRAMRAIRARFTGWRSRQGISYADADGDGYIDD 77

Query: 83  SEIENLVAFAQKTLGMKVTAW 103
            E++ L+ +AQK+LG+++ A+
Sbjct: 78  GEVDGLIEYAQKSLGLRIVAY 98



>Os02g0762900 EF-Hand type domain containing protein
          Length = 100

 Score = 75.5 bits (184), Expect = 9e-15,   Method: Compositional matrix adjust.
 Identities = 39/86 (45%), Positives = 53/86 (61%), Gaps = 1/86 (1%)

Query: 16  RGIPSSREMTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWFSGLKAGRAVRHADRD 75
           R +P   E TV++F+ WL QFD + DGRI R EL  A+R    WF+  KA  A+R AD +
Sbjct: 8   RALPQG-ETTVEEFRAWLSQFDANGDGRIGREELERALRSLNLWFAWWKAREAMREADAN 66

Query: 76  NSGFVDESEIENLVAFAQKTLGMKVT 101
            +G VD  E+  L AFAQ+ L +K+ 
Sbjct: 67  RNGVVDRDEMVRLYAFAQRHLHLKMN 92



>Os06g0213300 
          Length = 99

 Score = 72.0 bits (175), Expect = 9e-14,   Method: Compositional matrix adjust.
 Identities = 40/88 (45%), Positives = 54/88 (61%), Gaps = 2/88 (2%)

Query: 15  IRGIPSSREMTVDDFKNWLKQFDTDNDGRISRGELREAIRRRGGWFSGLKAGRAVRHADR 74
            R +P   E+TV++F+ WL QFD D DGRISR EL+ A+R    WF+  KA   VR AD 
Sbjct: 6   YRALPQG-EVTVEEFRAWLGQFDADGDGRISRDELQRALRSLNLWFAWWKARAGVRAADA 64

Query: 75  DNSGFV-DESEIENLVAFAQKTLGMKVT 101
           +  G V  + E+  L AFAQ+ L +K+ 
Sbjct: 65  NRDGAVAGDDEVATLFAFAQRHLNVKIA 92



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,414,405
Number of extensions: 137469
Number of successful extensions: 706
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 705
Number of HSP's successfully gapped: 6
Length of query: 103
Length of database: 17,035,801
Length adjustment: 71
Effective length of query: 32
Effective length of database: 13,328,607
Effective search space: 426515424
Effective search space used: 426515424
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)