BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os07g0587400 Os07g0587400|AK103740
(437 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os07g0587400 Similar to Eukaryotic peptide chain release fa... 902 0.0
Os03g0702800 Similar to Eukaryotic peptide chain release fa... 868 0.0
Os01g0939500 Similar to Eukaryotic peptide chain release fa... 800 0.0
Os05g0373900 Similar to Eukaryotic peptide chain release fa... 733 0.0
>Os07g0587400 Similar to Eukaryotic peptide chain release factor subunit 1-3
(eRF1-3) (Eukaryotic release factor 1-3) (Omnipotent
suppressor protein 1 homolog 3) (SUP1 homolog 3)
Length = 437
Score = 902 bits (2331), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 437/437 (100%), Positives = 437/437 (100%)
Query: 1 MSDSHETDRNIEIWKIKKLIKALESARGNGTSMISLIMPPRDQIARVAKMLGDEYGTASN 60
MSDSHETDRNIEIWKIKKLIKALESARGNGTSMISLIMPPRDQIARVAKMLGDEYGTASN
Sbjct: 1 MSDSHETDRNIEIWKIKKLIKALESARGNGTSMISLIMPPRDQIARVAKMLGDEYGTASN 60
Query: 61 IKSRVNRQSVLAAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFKPIN 120
IKSRVNRQSVLAAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFKPIN
Sbjct: 61 IKSRVNRQSVLAAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFKPIN 120
Query: 121 VSLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180
VSLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG
Sbjct: 121 VSLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180
Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS 240
RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS 240
Query: 241 DMFDQRLQAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKKLIGKYFEEISQDTG 300
DMFDQRLQAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKKLIGKYFEEISQDTG
Sbjct: 241 DMFDQRLQAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKKLIGKYFEEISQDTG 300
Query: 301 KYVFGVDDTLKALEMGAVETLIVWENLETNRYVLKNSASGETVIKHFNKEQEADQSNFRD 360
KYVFGVDDTLKALEMGAVETLIVWENLETNRYVLKNSASGETVIKHFNKEQEADQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVETLIVWENLETNRYVLKNSASGETVIKHFNKEQEADQSNFRD 360
Query: 361 PASNAELEVQEKMSLLEWFANEYKKFGCSLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
PASNAELEVQEKMSLLEWFANEYKKFGCSLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 PASNAELEVQEKMSLLEWFANEYKKFGCSLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
Query: 421 RSFDELSDDEGLYEDSD 437
RSFDELSDDEGLYEDSD
Sbjct: 421 RSFDELSDDEGLYEDSD 437
>Os03g0702800 Similar to Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1) (TB3-1) (Cl1 protein)
Length = 437
Score = 868 bits (2244), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 416/437 (95%), Positives = 427/437 (97%)
Query: 1 MSDSHETDRNIEIWKIKKLIKALESARGNGTSMISLIMPPRDQIARVAKMLGDEYGTASN 60
MSD ETD+NIEIWKIKKLIKALESARGNGTSMISLIMPPRDQ++RV KMLGDEYGTASN
Sbjct: 1 MSDGQETDKNIEIWKIKKLIKALESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60
Query: 61 IKSRVNRQSVLAAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFKPIN 120
IKSRVNRQSVLAAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFKPIN
Sbjct: 61 IKSRVNRQSVLAAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFKPIN 120
Query: 121 VSLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180
SLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTRE+LHKFTVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREILHKFTVDLPKKHG 180
Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS 240
RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS 240
Query: 241 DMFDQRLQAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKKLIGKYFEEISQDTG 300
DMFDQRLQAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKKLIGKYFEEISQDTG
Sbjct: 241 DMFDQRLQAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKKLIGKYFEEISQDTG 300
Query: 301 KYVFGVDDTLKALEMGAVETLIVWENLETNRYVLKNSASGETVIKHFNKEQEADQSNFRD 360
KYVFGVDDTLK LEMGAVETLIVWENL+ NRYVLKNSA+GET IKH NKEQEADQSNFRD
Sbjct: 301 KYVFGVDDTLKTLEMGAVETLIVWENLDVNRYVLKNSATGETAIKHLNKEQEADQSNFRD 360
Query: 361 PASNAELEVQEKMSLLEWFANEYKKFGCSLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
++NAELEVQ+K SLLEWFANEYKKFGC+LEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 TSTNAELEVQDKTSLLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
Query: 421 RSFDELSDDEGLYEDSD 437
RSFDELSDDEG+YEDSD
Sbjct: 421 RSFDELSDDEGVYEDSD 437
>Os01g0939500 Similar to Eukaryotic peptide chain release factor subunit 1-2
(eRF1-2) (Eukaryotic release factor 1-2) (Omnipotent
suppressor protein 1 homolog 2) (SUP1 homolog 2)
Length = 436
Score = 800 bits (2066), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 376/430 (87%), Positives = 409/430 (95%)
Query: 1 MSDSHETDRNIEIWKIKKLIKALESARGNGTSMISLIMPPRDQIARVAKMLGDEYGTASN 60
M+DSHETD+NIEIWK+KKLIK L++ARGNGTSMISLIMPPRDQ++RV KMLGDEYGTASN
Sbjct: 1 MADSHETDKNIEIWKVKKLIKGLDAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60
Query: 61 IKSRVNRQSVLAAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFKPIN 120
IKSRVNRQSVLAAITSAQQRLKLYN+VPPNGLVLYTGTIVT++GKEKKVT+DFEPF+PIN
Sbjct: 61 IKSRVNRQSVLAAITSAQQRLKLYNRVPPNGLVLYTGTIVTDEGKEKKVTVDFEPFRPIN 120
Query: 121 VSLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180
SLYLCDNKFHTEALNELLESDDKFGFI+MDGNGTLFGTLSGN+REVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIIMDGNGTLFGTLSGNSREVLHKFSVDLPKKHG 180
Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS 240
RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLI+AGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLIIAGSADFKTELSQS 240
Query: 241 DMFDQRLQAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKKLIGKYFEEISQDTG 300
DMFDQRLQAKIL VVDVSYGGENGFNQAIE+SAE L+NVKFIQEKKLIGKYFEEISQDTG
Sbjct: 241 DMFDQRLQAKILKVVDVSYGGENGFNQAIEISAEELSNVKFIQEKKLIGKYFEEISQDTG 300
Query: 301 KYVFGVDDTLKALEMGAVETLIVWENLETNRYVLKNSASGETVIKHFNKEQEADQSNFRD 360
KYVF VDDT+ ALEMGAVETLIVWENL+ NRYVLKNS +GET +KH NK QE DQSNFRD
Sbjct: 301 KYVFSVDDTMSALEMGAVETLIVWENLDINRYVLKNSVTGETTVKHLNKAQETDQSNFRD 360
Query: 361 PASNAELEVQEKMSLLEWFANEYKKFGCSLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
A++AELEV EK LLEWFA Y++FGCSLEFVTNKSQEGSQF RGFGGIGGILRYQ++I
Sbjct: 361 KATSAELEVIEKTLLLEWFAENYRQFGCSLEFVTNKSQEGSQFVRGFGGIGGILRYQVEI 420
Query: 421 RSFDELSDDE 430
++++LSD+E
Sbjct: 421 NAYEDLSDEE 430
>Os05g0373900 Similar to Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1) (TB3-1) (Cl1 protein)
Length = 440
Score = 733 bits (1891), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 339/424 (79%), Positives = 392/424 (92%)
Query: 1 MSDSHETDRNIEIWKIKKLIKALESARGNGTSMISLIMPPRDQIARVAKMLGDEYGTASN 60
M + HETD+NIE+WK+KKLIKAL++ARGNGTSMISLIMPPRDQ++RV KMLGDEYGTASN
Sbjct: 1 MGEGHETDKNIEVWKVKKLIKALDAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60
Query: 61 IKSRVNRQSVLAAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFKPIN 120
IKSRVNRQSVLAAITSAQQRLKLY++VP NGLVLYTGTIVT+DGKEKKVT DFEPF+PIN
Sbjct: 61 IKSRVNRQSVLAAITSAQQRLKLYSRVPVNGLVLYTGTIVTDDGKEKKVTFDFEPFRPIN 120
Query: 121 VSLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180
SLYLCDNKFHTEALNELLESDDKFGFI+MDGNGTL+GTLSGN+REVL+KF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIIMDGNGTLYGTLSGNSREVLYKFSVDLPKKHG 180
Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS 240
RGGQSA+RFARLRME+RHNY+RK AELATQFFIN AT+QPN++GLILAGSADFKTEL +S
Sbjct: 181 RGGQSAVRFARLRMERRHNYLRKAAELATQFFINSATNQPNIAGLILAGSADFKTELGKS 240
Query: 241 DMFDQRLQAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKKLIGKYFEEISQDTG 300
+MFD RLQAK++ +DVSYGGE+GFNQAIE+SAE+L++VKF+QEKKLIGKYFEEISQDTG
Sbjct: 241 EMFDPRLQAKVIKTLDVSYGGESGFNQAIEMSAEVLSDVKFVQEKKLIGKYFEEISQDTG 300
Query: 301 KYVFGVDDTLKALEMGAVETLIVWENLETNRYVLKNSASGETVIKHFNKEQEADQSNFRD 360
KYV GV DT+ ALE+GAV+TLIVWENL+ RY LKN+A+GETVIK+ N +QEADQSNF D
Sbjct: 301 KYVLGVQDTITALEIGAVDTLIVWENLDVRRYELKNTATGETVIKYLNSDQEADQSNFVD 360
Query: 361 PASNAELEVQEKMSLLEWFANEYKKFGCSLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
A++ E +V +K LLEWFA Y+++GC+LEFVTNKSQEGSQFCRGFGGIGGILRY D+
Sbjct: 361 EATSGEFDVIDKPLLLEWFAENYQQYGCTLEFVTNKSQEGSQFCRGFGGIGGILRYPADV 420
Query: 421 RSFD 424
+F+
Sbjct: 421 AAFN 424
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.316 0.134 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 14,064,319
Number of extensions: 555286
Number of successful extensions: 1218
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1218
Number of HSP's successfully gapped: 4
Length of query: 437
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 333
Effective length of database: 11,605,545
Effective search space: 3864646485
Effective search space used: 3864646485
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 157 (65.1 bits)