BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os06g0683400 Os06g0683400|AK112082
(125 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os06g0683400 Similar to EF-hand Ca2+-binding protein CCD1 182 4e-47
Os02g0122600 EF-Hand type domain containing protein 107 2e-24
Os01g0783700 Similar to EF-hand Ca2+-binding protein CCD1 104 2e-23
>Os06g0683400 Similar to EF-hand Ca2+-binding protein CCD1
Length = 125
Score = 182 bits (463), Expect = 4e-47, Method: Compositional matrix adjust.
Identities = 95/111 (85%), Positives = 95/111 (85%)
Query: 15 GFEDYLPVMAERLGEEGLMQELASGFRLLMDPASGLITFDSLRRNAPXXXXXXXXXXXXX 74
GFEDYLPVMAERLGEEGLMQELASGFRLLMDPASGLITFDSLRRNAP
Sbjct: 15 GFEDYLPVMAERLGEEGLMQELASGFRLLMDPASGLITFDSLRRNAPLLGLGGMSDDDLR 74
Query: 75 XXXAEGDFDGDGALSEMEFCVLMVRLSPDLMDEPRRWLDDAVAQASQFLFH 125
AEGDFDGDGALSEMEFCVLMVRLSPDLMDEPRRWLDDAVAQASQFLFH
Sbjct: 75 GMLAEGDFDGDGALSEMEFCVLMVRLSPDLMDEPRRWLDDAVAQASQFLFH 125
>Os02g0122600 EF-Hand type domain containing protein
Length = 147
Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust.
Identities = 58/107 (54%), Positives = 69/107 (64%), Gaps = 5/107 (4%)
Query: 16 FEDYLPVMAERLGEEGLMQELASGFRLLMDPASGLITFDSLRRNAPXXXXXXXXXXXXXX 75
+ED +PVMA RLG EGL+ EL +GFRLL DPA G IT +SLRR+A
Sbjct: 28 YEDLMPVMAGRLGAEGLLSELRAGFRLLADPARGAITAESLRRSAASVLGLGGGGGEMTV 87
Query: 76 XXA-----EGDFDGDGALSEMEFCVLMVRLSPDLMDEPRRWLDDAVA 117
A EGD DGDGALSE EFCVLMVRLSP +M + WL++A+A
Sbjct: 88 EEAAAMVREGDQDGDGALSEAEFCVLMVRLSPGIMGDAEGWLEEAIA 134
>Os01g0783700 Similar to EF-hand Ca2+-binding protein CCD1
Length = 111
Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 56/96 (58%), Positives = 64/96 (66%), Gaps = 1/96 (1%)
Query: 16 FEDYLPVMAERLGEEGLMQELASGFRLLMDPASGLITFDSLRRNAPXXXXXXXXXXXXXX 75
FED+LP MA +LG EGL++EL GF LLMDP +G ITF SL+RNA
Sbjct: 12 FEDFLPSMARKLGVEGLIEELCKGFELLMDPGAGKITFRSLKRNAAMLGLGELRDDELSE 71
Query: 76 XXAEGDFDGDGALSEMEFCVLMVRLSPDLM-DEPRR 110
EGD DGDGAL +MEFCVLMVRLSP+LM DE R
Sbjct: 72 MMREGDLDGDGALDQMEFCVLMVRLSPELMQDESHR 107
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.323 0.141 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,298,587
Number of extensions: 100482
Number of successful extensions: 224
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 221
Number of HSP's successfully gapped: 3
Length of query: 125
Length of database: 17,035,801
Length adjustment: 88
Effective length of query: 37
Effective length of database: 12,440,969
Effective search space: 460315853
Effective search space used: 460315853
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)