BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0223700 Os06g0223700|AK070214
         (142 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0223700  Protein of unknown function DUF581 family protein   199   7e-52
Os08g0407600  Protein of unknown function DUF581 family protein    94   3e-20
Os02g0751300  Protein of unknown function DUF581 family protein    78   2e-15

>Os06g0223700 Protein of unknown function DUF581 family protein
          Length = 142

 Score =  199 bits (505), Expect = 7e-52,   Method: Compositional matrix adjust.
 Identities = 102/142 (71%), Positives = 102/142 (71%)

Query: 1   MTTLGKRHRNNSSMRRTTSMSGFAVAEEEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXMQ 60
           MTTLGKRHRNNSSMRRTTSMSGFAVAEEEE                            MQ
Sbjct: 1   MTTLGKRHRNNSSMRRTTSMSGFAVAEEEEQQGRQPPARAARGGGGGGSAAPSPGWGAMQ 60

Query: 61  RRHSGDFAVAETAAFLKACGICNRRLGPGRDTFIYMGEVAFCSHECRQQQMNLDELNEKK 120
           RRHSGDFAVAETAAFLKACGICNRRLGPGRDTFIYMGEVAFCSHECRQQQMNLDELNEKK
Sbjct: 61  RRHSGDFAVAETAAFLKACGICNRRLGPGRDTFIYMGEVAFCSHECRQQQMNLDELNEKK 120

Query: 121 CFQREXXXXXXXXXXXXAVAAA 142
           CFQRE            AVAAA
Sbjct: 121 CFQREGGGGSDKSGNSGAVAAA 142



>Os08g0407600 Protein of unknown function DUF581 family protein
          Length = 199

 Score = 94.0 bits (232), Expect = 3e-20,   Method: Compositional matrix adjust.
 Identities = 40/60 (66%), Positives = 49/60 (81%)

Query: 61  RRHSGDFAVAETAAFLKACGICNRRLGPGRDTFIYMGEVAFCSHECRQQQMNLDELNEKK 120
           RR+S DF+  ETAAFL+ACG+CNRRLGPGRDTF+Y G+ AFCS ECRQQ +  +E  EK+
Sbjct: 103 RRNSADFSAVETAAFLRACGLCNRRLGPGRDTFMYKGDTAFCSLECRQQHITHEEWKEKR 162



>Os02g0751300 Protein of unknown function DUF581 family protein
          Length = 147

 Score = 77.8 bits (190), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 44/118 (37%), Positives = 56/118 (47%), Gaps = 7/118 (5%)

Query: 4   LGKRHRNNSSMRRTTSMSGFAVAEEEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXMQRRH 63
           LGKR R+  +M RTTSM+    A ++                                  
Sbjct: 6   LGKRQRSQGTMHRTTSMASVPAAAKQ-------GRRHVVEGPPRAPPASLLAGGGPATAA 58

Query: 64  SGDFAVAETAAFLKACGICNRRLGPGRDTFIYMGEVAFCSHECRQQQMNLDELNEKKC 121
           + D    ETAAFLK C +C R LGPG+DT+IY GEVAFCS ECR+  +   E  E+ C
Sbjct: 59  AADHGGVETAAFLKNCALCGRDLGPGKDTYIYRGEVAFCSKECRECVIEYYERKERNC 116



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.130    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,169,365
Number of extensions: 82008
Number of successful extensions: 186
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 187
Number of HSP's successfully gapped: 3
Length of query: 142
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 51
Effective length of database: 12,284,327
Effective search space: 626500677
Effective search space used: 626500677
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 151 (62.8 bits)