BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0531100 Os05g0531100|AK109325
(142 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os05g0531100 Protein of unknown function DUF584 family protein 223 3e-59
Os01g0748300 Protein of unknown function DUF584 family protein 81 3e-16
Os01g0727700 Protein of unknown function DUF584 family protein 71 3e-13
>Os05g0531100 Protein of unknown function DUF584 family protein
Length = 142
Score = 223 bits (569), Expect = 3e-59, Method: Compositional matrix adjust.
Identities = 112/142 (78%), Positives = 112/142 (78%)
Query: 1 MEEFQEADILWPEPAEDNSDDGXXXXXXXXXXXXXXXXGSPESSSLSAPVEIXXXXXXXX 60
MEEFQEADILWPEPAEDNSDDG GSPESSSLSAPVEI
Sbjct: 1 MEEFQEADILWPEPAEDNSDDGAVVVTTTPSPVARRPVGSPESSSLSAPVEIAASRRKRR 60
Query: 61 XXXXXXEYNMFDQTNDDDDAVKKKMMNNGVMVAPPHAIVDRRRLRGRTAAYSMCAGKGRT 120
EYNMFDQTNDDDDAVKKKMMNNGVMVAPPHAIVDRRRLRGRTAAYSMCAGKGRT
Sbjct: 61 SRSWASEYNMFDQTNDDDDAVKKKMMNNGVMVAPPHAIVDRRRLRGRTAAYSMCAGKGRT 120
Query: 121 LKGRDLRNVRNLVLQMTGFIEK 142
LKGRDLRNVRNLVLQMTGFIEK
Sbjct: 121 LKGRDLRNVRNLVLQMTGFIEK 142
>Os01g0748300 Protein of unknown function DUF584 family protein
Length = 142
Score = 80.9 bits (198), Expect = 3e-16, Method: Compositional matrix adjust.
Identities = 56/144 (38%), Positives = 62/144 (43%), Gaps = 13/144 (9%)
Query: 1 MEEFQEADILWPEPAEDNSDDGXXXXXXXXXXXXXXXXGSPESSSLSAPVEIXXXXXXXX 60
MEE QEAD+LWP AE + D SS SAPV I
Sbjct: 1 MEELQEADVLWP--AEHHRDYERCRHHHHAAQPAVCPVAPLRPSSSSAPVRIPAPTTTTF 58
Query: 61 XXXXXXEYNMFDQTNDDDDAVKKKMMNNGVM--VAPPHAIVD--RRRLRGRTAAYSMCAG 116
Y +DD G + PPH V RR GRT A S+C G
Sbjct: 59 AGARRGYY-------EDDGTRTDYSAGAGASSRIVPPHVYVAAARRGSEGRTVASSVCVG 111
Query: 117 KGRTLKGRDLRNVRNLVLQMTGFI 140
GRTLKGRDLR VRN VL MTGF+
Sbjct: 112 HGRTLKGRDLRAVRNAVLHMTGFL 135
>Os01g0727700 Protein of unknown function DUF584 family protein
Length = 148
Score = 70.9 bits (172), Expect = 3e-13, Method: Compositional matrix adjust.
Identities = 33/50 (66%), Positives = 40/50 (80%), Gaps = 5/50 (10%)
Query: 92 VAPPHAIVDRRRLRGRTAAYSMCAGKGRTLKGRDLRNVRNLVLQMTGFIE 141
V PPH ++ RRR AA+SMC+G+GRTLKGRDLR VR+ VL+MTGFIE
Sbjct: 103 VVPPHVLLARRR-----AAFSMCSGQGRTLKGRDLRRVRDSVLRMTGFIE 147
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.314 0.132 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,149,676
Number of extensions: 134695
Number of successful extensions: 199
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 201
Number of HSP's successfully gapped: 3
Length of query: 142
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 51
Effective length of database: 12,284,327
Effective search space: 626500677
Effective search space used: 626500677
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 151 (62.8 bits)