BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0512000 Os05g0512000|AK102433
         (987 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0512000  Zinc finger, RING-type domain containing protein   1943   0.0  
Os04g0512400  Similar to Midline 1 protein (Tripartite motif...   299   5e-81
Os05g0486600                                                      118   3e-26

>Os05g0512000 Zinc finger, RING-type domain containing protein
          Length = 987

 Score = 1943 bits (5033), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 942/987 (95%), Positives = 942/987 (95%)

Query: 1   MADTGSLEKMGRELKCPICLSLLSSAVSISCNHVFCNDCLTESMKSTSSCPVCKVPFRRR 60
           MADTGSLEKMGRELKCPICLSLLSSAVSISCNHVFCNDCLTESMKSTSSCPVCKVPFRRR
Sbjct: 1   MADTGSLEKMGRELKCPICLSLLSSAVSISCNHVFCNDCLTESMKSTSSCPVCKVPFRRR 60

Query: 61  EMRPAPHMDNLVSIFKSMEAAAGTNVVSTQEAPVVKLADGSDCVNSGKNSKRSQKSLTRK 120
           EMRPAPHMDNLVSIFKSMEAAAGTNVVSTQEAPVVKLADGSDCVNSGKNSKRSQKSLTRK
Sbjct: 61  EMRPAPHMDNLVSIFKSMEAAAGTNVVSTQEAPVVKLADGSDCVNSGKNSKRSQKSLTRK 120

Query: 121 RKVTSEMEKNTAKDATASASQPTTKPSFSTNKRIQVKPFPESETPIRAEKIMKPEEPKNN 180
           RKVTSEMEKNTAKDATASASQPTTKPSFSTNKRIQVKPFPESETPIRAEKIMKPEEPKNN
Sbjct: 121 RKVTSEMEKNTAKDATASASQPTTKPSFSTNKRIQVKPFPESETPIRAEKIMKPEEPKNN 180

Query: 181 LNNDVEGKNKAVASGQPGSPSLSPFFWLREQEEQEGCTAETLSETQSLDTPLRHNAPSFS 240
           LNNDVEGKNKAVASGQPGSPSLSPFFWLREQEEQEGCTAETLSETQSLDTPLRHNAPSFS
Sbjct: 181 LNNDVEGKNKAVASGQPGSPSLSPFFWLREQEEQEGCTAETLSETQSLDTPLRHNAPSFS 240

Query: 241 DIKDSDDEIPLNTTPNSKAAATELFDSEIFEWTQRPCSPELYSTPLKKQSKAKSKLDQIE 300
           DIKDSDDEIPLNTTPNSKAAATELFDSEIFEWTQRPCSPELYSTPLKKQSKAKSKLDQIE
Sbjct: 241 DIKDSDDEIPLNTTPNSKAAATELFDSEIFEWTQRPCSPELYSTPLKKQSKAKSKLDQIE 300

Query: 301 EKGDEEDVHIGGSFDKLGNASNAAQLVNTKATKQKRKKTSPSNKNSAKLSNRAEPCIKKS 360
           EKGDEEDVHIGGSFDKLGNASNAAQLVNTKATKQKRKKTSPSNKNSAKLSNRAEPCIKKS
Sbjct: 301 EKGDEEDVHIGGSFDKLGNASNAAQLVNTKATKQKRKKTSPSNKNSAKLSNRAEPCIKKS 360

Query: 361 DANQQGSNRRKSAALKSCQKSSSAVGRNTSGRRNKASSNSKPIHGSSDNSPESYLPKEGL 420
           DANQQGSNRRKSAALKSCQKSSSAVGRNTSGRRNKASSNSKPIHGSSDNSPESYLPKEGL
Sbjct: 361 DANQQGSNRRKSAALKSCQKSSSAVGRNTSGRRNKASSNSKPIHGSSDNSPESYLPKEGL 420

Query: 421 DVEAPDKPLSERIQNLEKTSRRKGSARKLEMAGKTISDTTEKNSEPRSKRVRRMSDHAIA 480
           DVEAPDKPLSERIQNLEKTSRRKGSARKLEMAGKTISDTTEKNSEPRSKRVRRMSDHAIA
Sbjct: 421 DVEAPDKPLSERIQNLEKTSRRKGSARKLEMAGKTISDTTEKNSEPRSKRVRRMSDHAIA 480

Query: 481 KPVEVPSGSGNETEIPQLHTLTKGSIQRKSSNARRHSKVCGEQEGKNKLENTTMTPIILH 540
           KPVEVPSGSGNETEIPQLHTLTKGSIQRKSSNARRHSKVCGEQEGKNKLENTTMTPIILH
Sbjct: 481 KPVEVPSGSGNETEIPQLHTLTKGSIQRKSSNARRHSKVCGEQEGKNKLENTTMTPIILH 540

Query: 541 GKCQNKEAVCTAPSVRTASVKYKQAKFSEQPDCFGTENFGNLQACPARNVLLKKCEVSTL 600
           GKCQNKEAVCTAPSVRTASVKYKQAKFSEQPDCFGTENFGNLQACPARNVLLKKCEVSTL
Sbjct: 541 GKCQNKEAVCTAPSVRTASVKYKQAKFSEQPDCFGTENFGNLQACPARNVLLKKCEVSTL 600

Query: 601 KVSCAFCQTDVITEESGEMVHYQNGKQVPAEFNGGANVVHSHKNCLEWAPDVYFEDDSAF 660
           KVSCAFCQTDVITEESGEMVHYQNGKQVPAEFNGGANVVHSHKNCLEWAPDVYFEDDSAF
Sbjct: 601 KVSCAFCQTDVITEESGEMVHYQNGKQVPAEFNGGANVVHSHKNCLEWAPDVYFEDDSAF 660

Query: 661 NLTTELARSRRIKCACCGIKGAALGCFEMSCRRSFHFTCAKLIPECRWDNENFVMLCPLH 720
           NLTTELARSRRIKCACCGIKGAALGCFEMSCRRSFHFTCAKLIPECRWDNENFVMLCPLH
Sbjct: 661 NLTTELARSRRIKCACCGIKGAALGCFEMSCRRSFHFTCAKLIPECRWDNENFVMLCPLH 720

Query: 721 RSTKLPNENSEQQKQPKRKTTLKGSSQIGSNQDCGNNWKWPSGSPQKWXXXXXXXXXXXX 780
           RSTKLPNENSEQQKQPKRKTTLKGSSQIGSNQDCGNNWKWPSGSPQKW            
Sbjct: 721 RSTKLPNENSEQQKQPKRKTTLKGSSQIGSNQDCGNNWKWPSGSPQKWVLCCSSLSSSEK 780

Query: 781 XXXXXFAKLAGVPISATWSPNVTHVIASTDLSGACKRTLKFLMAILNGRWIVSIDWVKTC 840
                FAKLAGVPISATWSPNVTHVIASTDLSGACKRTLKFLMAILNGRWIVSIDWVKTC
Sbjct: 781 ELVSEFAKLAGVPISATWSPNVTHVIASTDLSGACKRTLKFLMAILNGRWIVSIDWVKTC 840

Query: 841 MECMEPIDEHKFEVATDVHGITDGPRLGRCRVIDRQPKLFDSMRFYLHGDYTKSYRGYLQ 900
           MECMEPIDEHKFEVATDVHGITDGPRLGRCRVIDRQPKLFDSMRFYLHGDYTKSYRGYLQ
Sbjct: 841 MECMEPIDEHKFEVATDVHGITDGPRLGRCRVIDRQPKLFDSMRFYLHGDYTKSYRGYLQ 900

Query: 901 DLVVAAGGIVLQRKPVSRXXXXXXXXXXXXXIVYSFENQDRAKSKAETKXXXXXXXXXXX 960
           DLVVAAGGIVLQRKPVSR             IVYSFENQDRAKSKAETK           
Sbjct: 901 DLVVAAGGIVLQRKPVSRDQQKLLDDSSDLLIVYSFENQDRAKSKAETKAADRRQADAQA 960

Query: 961 XXXXSGGRVVSSAWVIDSIAACNLQPL 987
               SGGRVVSSAWVIDSIAACNLQPL
Sbjct: 961 LACASGGRVVSSAWVIDSIAACNLQPL 987



>Os04g0512400 Similar to Midline 1 protein (Tripartite motif protein 18)
          Length = 629

 Score =  299 bits (766), Expect = 5e-81,   Method: Compositional matrix adjust.
 Identities = 155/391 (39%), Positives = 211/391 (53%), Gaps = 23/391 (5%)

Query: 604 CAFCQTDVITEESGEMVHYQNGKQVPAEFNGGANVVHSHKNCLEWAPDVYFEDDSAFNLT 663
           C FC +   TE +G + HY +G+ +        NV+H H+ C+EWAP  +F  D A NL 
Sbjct: 250 CEFCHSSKTTESTGPLSHYLHGEPLEDNQAWKPNVLHVHEKCIEWAPQAFFTGDIANNLE 309

Query: 664 TELARSRRIKCACCGIKGAALGCFEMSCRRSFHFTCAKLIPECRWDNENFVMLCPLHRST 723
            ELAR+ +IKC+ CG+KGAALGC   SCR+SFH  CA  I  CRWD+ENFVMLCP H S 
Sbjct: 310 PELARASKIKCSVCGLKGAALGCLVKSCRKSFHVPCAHGISGCRWDDENFVMLCPSHSSK 369

Query: 724 KLPNENSEQQKQPKRKTTLKGSS------QIGSNQDCGNNWKWPSGSP---QKWXXXXXX 774
           KLP E S   K   +KT+L+ SS       + S      +  W + SP    +W      
Sbjct: 370 KLPCERS---KSKNKKTSLQRSSSDTMLDDLNSPSTIHMDGLW-TASPFLTSEWVICGSA 425

Query: 775 XXXXXXXXXXXFAKLAGVPISATWSPNVTHVIASTDLSGACKRTLKFLMAILNGRWIVSI 834
                      F    G+ ++  W  NVTHVIA+TD  GAC RTLK LMAIL G+W+++I
Sbjct: 426 LSSQEKEILDQFEHQTGITVTNGWRSNVTHVIANTDECGACARTLKVLMAILAGKWVLNI 485

Query: 835 DWVKTCMECMEPIDEHKFEVATDVHGITDGPRLGRCRVIDRQPKLFDSMRFYLHGDYTKS 894
           +W+K CME  EP+ E  +E+++DVHG  DGPR+GR R +   P LF  + FY  G +  +
Sbjct: 486 NWLKACMEAKEPVPEEPYEISSDVHGSFDGPRMGRLRAMQNAPHLFAGLTFYFSGHFMPN 545

Query: 895 YRGYLQDLVVAAGGIVLQRKPVSRXXXXXXXXXXXXXIVYSFENQDRAKSKAETKXXXXX 954
           Y+ +L+DL+ AAGG +L +  +S              I+YS E    +      +     
Sbjct: 546 YKVHLEDLITAAGGSILDKADLS----------STSLIIYSMEPPQGSDPDTLNEVIRKR 595

Query: 955 XXXXXXXXXXSGGRVVSSAWVIDSIAACNLQ 985
                      G R V    V+DSIA+C +Q
Sbjct: 596 KAEAEELAATIGSRAVPHTCVLDSIASCTVQ 626




 Score = 72.8 bits (177), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 35/94 (37%), Positives = 57/94 (60%), Gaps = 5/94 (5%)

Query: 7   LEKMGRELKCPICLSLLSSAVSISCNHVFCNDCLTESMKSTSSCPVCKVPFRRREMRPAP 66
           L+KM  EL CP+CL LL++   + C H  C+ C T       SC +CK  +R +++RPA 
Sbjct: 15  LQKMELELTCPVCLKLLNAPTMLPCYHTSCSKCATTRTMDGYSCAICKSAYRSQDLRPAS 74

Query: 67  HMDNLVSIFKSMEAAAGTNVVSTQEA----PVVK 96
           H++ +V+I +S+ +   +++V+ QEA    PV K
Sbjct: 75  HLEAIVNIHRSLSSTV-SSMVTQQEAQADIPVAK 107



>Os05g0486600 
          Length = 556

 Score =  118 bits (295), Expect = 3e-26,   Method: Compositional matrix adjust.
 Identities = 56/146 (38%), Positives = 84/146 (57%), Gaps = 7/146 (4%)

Query: 587 ARNVLLKKCE--VSTLKVSCAFCQT-DVITEESGEMVHYQNGKQVPAEFNGG---ANVVH 640
            R   LK+ +  ++  +  C FC +     +  G M HYQ GK V ++ NG     ++++
Sbjct: 68  GRATRLKRLQTPINLFEDECVFCHSFRTPPQFHGPMAHYQKGKLVSSD-NGSPCPTDIIY 126

Query: 641 SHKNCLEWAPDVYFEDDSAFNLTTELARSRRIKCACCGIKGAALGCFEMSCRRSFHFTCA 700
            HK CLEWAP V++E D+  NL  E+ R+ ++KC  C + GAALGC+   C RS+H  CA
Sbjct: 127 VHKKCLEWAPQVFYEGDTIVNLELEIKRAAKLKCKRCKLPGAALGCYYTKCNRSYHVPCA 186

Query: 701 KLIPECRWDNENFVMLCPLHRSTKLP 726
            +   CRWD +N  ++CP H +  LP
Sbjct: 187 MMTLNCRWDVDNGCVMCPEHATMPLP 212




 Score = 74.7 bits (182), Expect = 4e-13,   Method: Compositional matrix adjust.
 Identities = 51/204 (25%), Positives = 80/204 (39%), Gaps = 2/204 (0%)

Query: 786 FAKLAGVPISATWSPNVTHVIASTDLSGACKRTLKFLMAILNGRWIVSIDWVKTCMECME 845
           FA      +   W+ NVTHVI       A  R+ + LMA+L G+W+V++ W+  C+    
Sbjct: 343 FASWTDATVVNEWTENVTHVIVGKSAGSAWSRSYEVLMALLFGKWVVTVGWIMDCLVKFI 402

Query: 846 PIDEHKFEV--ATDVHGITDGPRLGRCRVIDRQPKLFDSMRFYLHGDYTKSYRGYLQDLV 903
           P  E  FE+  + D      G +  R +  +   KLF  + F L        R ++Q L+
Sbjct: 403 PSPEASFELRFSHDSRASIGGNKKRRNQASEGAQKLFSGLNFCLSAYINPDDRQHIQSLI 462

Query: 904 VAAGGIVLQRKPVSRXXXXXXXXXXXXXIVYSFENQDRAKSKAETKXXXXXXXXXXXXXX 963
            AAGG +L+    S                + ++                          
Sbjct: 463 AAAGGQILETNGGSHLLRENLEKVAVKPCYFVYDGGAPRDFTQGLLDDLPKEMEEGREYA 522

Query: 964 XSGGRVVSSAWVIDSIAACNLQPL 987
             G +V+S   V D+IAA + Q L
Sbjct: 523 ACGAQVISHLRVFDAIAAYDAQIL 546



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.126    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 33,195,948
Number of extensions: 1415047
Number of successful extensions: 4459
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 4454
Number of HSP's successfully gapped: 5
Length of query: 987
Length of database: 17,035,801
Length adjustment: 111
Effective length of query: 876
Effective length of database: 11,240,047
Effective search space: 9846281172
Effective search space used: 9846281172
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 161 (66.6 bits)