BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0496200 Os05g0496200|AK062214
         (181 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0496200  Similar to 3-phosphoglycerate kinase (Fragment)     350   3e-97
Os06g0668200  Similar to Phosphoglycerate kinase, cytosolic ...   293   5e-80
Os02g0169300  Similar to Phosphoglycerate kinase, cytosolic ...   278   2e-75
AK100371                                                          276   4e-75
Os01g0800266  Phosphoglycerate kinase family protein              190   5e-49
AK110279                                                           85   3e-17

>Os05g0496200 Similar to 3-phosphoglycerate kinase (Fragment)
          Length = 181

 Score =  350 bits (898), Expect = 3e-97,   Method: Compositional matrix adjust.
 Identities = 181/181 (100%), Positives = 181/181 (100%)

Query: 1   MIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKEKGVSLLLPSDVIIADKFAPDANSQV 60
           MIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKEKGVSLLLPSDVIIADKFAPDANSQV
Sbjct: 1   MIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKEKGVSLLLPSDVIIADKFAPDANSQV 60

Query: 61  VPASAIPDGWMGLDIGPDSVASFSSTLETTQTVIWNGPMGVFEFDKFAVGTEAIAKKLAD 120
           VPASAIPDGWMGLDIGPDSVASFSSTLETTQTVIWNGPMGVFEFDKFAVGTEAIAKKLAD
Sbjct: 61  VPASAIPDGWMGLDIGPDSVASFSSTLETTQTVIWNGPMGVFEFDKFAVGTEAIAKKLAD 120

Query: 121 LSGKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVIALDEAVTVA 180
           LSGKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVIALDEAVTVA
Sbjct: 121 LSGKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVIALDEAVTVA 180

Query: 181 V 181
           V
Sbjct: 181 V 181



>Os06g0668200 Similar to Phosphoglycerate kinase, cytosolic (EC 2.7.2.3)
          Length = 401

 Score =  293 bits (749), Expect = 5e-80,   Method: Compositional matrix adjust.
 Identities = 142/176 (80%), Positives = 162/176 (92%)

Query: 1   MIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKEKGVSLLLPSDVIIADKFAPDANSQV 60
           MI+TFYKAQG +VG SLVEEDKLELATSL+ KAK KGVSLLLP+D+++ADKFA DA S+ 
Sbjct: 226 MIYTFYKAQGYAVGKSLVEEDKLELATSLIEKAKSKGVSLLLPTDIVVADKFAADAESKT 285

Query: 61  VPASAIPDGWMGLDIGPDSVASFSSTLETTQTVIWNGPMGVFEFDKFAVGTEAIAKKLAD 120
           VPASAIPDGWMGLDIGPDS+ +FS TL+TT+TVIWNGPMGVFEF+KFA GT+AIAKKLAD
Sbjct: 286 VPASAIPDGWMGLDIGPDSIKTFSETLDTTKTVIWNGPMGVFEFEKFAAGTDAIAKKLAD 345

Query: 121 LSGKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVIALDEA 176
           ++ KGVTTIIGGGDSVAAVEK G+AD MSHISTGGGASLELLEGK LPGV+AL++A
Sbjct: 346 ITAKGVTTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKTLPGVLALNDA 401



>Os02g0169300 Similar to Phosphoglycerate kinase, cytosolic (EC 2.7.2.3)
          Length = 402

 Score =  278 bits (710), Expect = 2e-75,   Method: Compositional matrix adjust.
 Identities = 139/177 (78%), Positives = 156/177 (88%), Gaps = 1/177 (0%)

Query: 1   MIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKEKGVSLLLPSDVIIADKFAPDANSQV 60
           MIFTFYKAQG +VG SLVEEDKLELATSL+ KAK KGVSLLLP+DV++ADKFA DA S+ 
Sbjct: 226 MIFTFYKAQGYAVGKSLVEEDKLELATSLIEKAKAKGVSLLLPTDVVVADKFAADAESKT 285

Query: 61  VPASAIPDGWMGLDIGPDSVASFSSTLETTQTVIWNGPMGVFEFDKFAVGTEAIAKKLAD 120
           V ASAIPDGWMGLD+GPD++ + S  L+T  T+IWNGPMGVFEF+KFA GT+AIAKKLAD
Sbjct: 286 VAASAIPDGWMGLDVGPDAIKTSSEALDTCNTIIWNGPMGVFEFEKFAAGTDAIAKKLAD 345

Query: 121 L-SGKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVIALDEA 176
           L + KG TTIIGGGDSVAAVEK G+AD MSHISTGGGASLELLEGK LPGV+ALDEA
Sbjct: 346 LTTTKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKTLPGVLALDEA 402



>AK100371 
          Length = 365

 Score =  276 bits (707), Expect = 4e-75,   Method: Compositional matrix adjust.
 Identities = 139/177 (78%), Positives = 156/177 (88%), Gaps = 1/177 (0%)

Query: 1   MIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKEKGVSLLLPSDVIIADKFAPDANSQV 60
           MIFTFYKAQG +VG SLVEEDKLELATSL+ KAK KGVSLLLP+DV++ADKFA DA S+ 
Sbjct: 189 MIFTFYKAQGYAVGKSLVEEDKLELATSLIEKAKAKGVSLLLPTDVVVADKFAADAESKT 248

Query: 61  VPASAIPDGWMGLDIGPDSVASFSSTLETTQTVIWNGPMGVFEFDKFAVGTEAIAKKLAD 120
           V ASAIPDGWMGLD+GPD++ + S  L+T  T+IWNGPMGVFEF+KFA GT+AIAKKLAD
Sbjct: 249 VAASAIPDGWMGLDVGPDAIKTSSEALDTCNTIIWNGPMGVFEFEKFAAGTDAIAKKLAD 308

Query: 121 L-SGKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVIALDEA 176
           L + KG TTIIGGGDSVAAVEK G+AD MSHISTGGGASLELLEGK LPGV+ALDEA
Sbjct: 309 LTTTKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKTLPGVLALDEA 365



>Os01g0800266 Phosphoglycerate kinase family protein
          Length = 385

 Score =  190 bits (483), Expect = 5e-49,   Method: Compositional matrix adjust.
 Identities = 108/176 (61%), Positives = 118/176 (67%), Gaps = 53/176 (30%)

Query: 1   MIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKEKGVSLLLPSDVIIADKFAPDANSQV 60
           MIFTFYKAQG  VG+SLVE+DKLELATSLLAKAKEKGVSL+LP+DVI+ADKFAP+AN Q 
Sbjct: 263 MIFTFYKAQGFPVGASLVEDDKLELATSLLAKAKEKGVSLMLPTDVIVADKFAPEANCQA 322

Query: 61  VPASAIPDGWMGLDIGPDSVASFSSTLETTQTVIWNGPMGVFEFDKFAVGTEAIAKKLAD 120
           +                                                     AKKLA+
Sbjct: 323 I-----------------------------------------------------AKKLAE 329

Query: 121 LSGKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGVIALDEA 176
           LSGKGVTTIIGG DSVAAVEKVGVA+VMSHISTGGGASLELLEGKELPGV+ALDEA
Sbjct: 330 LSGKGVTTIIGGKDSVAAVEKVGVANVMSHISTGGGASLELLEGKELPGVVALDEA 385



>AK110279 
          Length = 844

 Score = 84.7 bits (208), Expect = 3e-17,   Method: Composition-based stats.
 Identities = 53/186 (28%), Positives = 89/186 (47%), Gaps = 17/186 (9%)

Query: 5   FYKAQGLSVGS----------SLVEEDK----LELATSLLAKAKEKGVSLLLPSDVIIAD 50
           F + +G+SVG            ++ ED      + A  ++  A+   V ++LP D +   
Sbjct: 250 FLQVKGISVGKLSPNICDTAGHIIWEDPSTSPQDFARKIMRLAERYNVKIVLPEDFVCNR 309

Query: 51  KFAPDANSQVVPASAIPDGWMGLDIGPDSVASFSSTLETTQTVIWNGPMGVFEFDKFAVG 110
              P     V P + +P     +DIGP ++  F++ +  + TV W G MG  +   +  G
Sbjct: 310 TIEPTRFPTVTPTNRVPADLYIVDIGPKTIQRFTAEISDSHTVFWTGRMGCADKADYTNG 369

Query: 111 TEAIAKKLADLSGKGVTTIIGGGDSVAAVEKVGVADVMSHISTGGGASLELLEGKELPGV 170
           T AIA  +         T++GG ++V AV   GV D ++H++TG  + L +LEG  L  +
Sbjct: 370 TTAIAIAVGH---SNCVTVVGGKNTVQAVRTAGVEDSINHMTTGPNSMLRVLEGTPLAAL 426

Query: 171 IALDEA 176
            AL +A
Sbjct: 427 NALSDA 432



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.132    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,052,384
Number of extensions: 256437
Number of successful extensions: 608
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 605
Number of HSP's successfully gapped: 7
Length of query: 181
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 86
Effective length of database: 12,075,471
Effective search space: 1038490506
Effective search space used: 1038490506
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 153 (63.5 bits)