BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0491700 Os05g0491700|Os05g0491700
         (188 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0491700  VQ domain containing protein                        213   6e-56
Os05g0390800  VQ domain containing protein                         68   3e-12
Os01g0808900  Similar to SigA binding protein                      68   3e-12

>Os05g0491700 VQ domain containing protein
          Length = 188

 Score =  213 bits (542), Expect = 6e-56,   Method: Compositional matrix adjust.
 Identities = 114/188 (60%), Positives = 114/188 (60%)

Query: 1   MDKKQCXXXXXXXXXXXXXXXXXXXXXMHXXXXXXXXXXXXXXXXXEIKVVYIANPMRVT 60
           MDKKQC                     MH                 EIKVVYIANPMRVT
Sbjct: 1   MDKKQCGAKGGGGGNARRSATGGGAGRMHRKGKHQGDGGGGGGKRREIKVVYIANPMRVT 60

Query: 61  TSEEGFRALVQELTGRHADPSKYRGGGGGAPVDETSXXXXXXXXXXXXXXXXXXXPSPGS 120
           TSEEGFRALVQELTGRHADPSKYRGGGGGAPVDETS                   PSPGS
Sbjct: 61  TSEEGFRALVQELTGRHADPSKYRGGGGGAPVDETSGGGGGGGGEMMQGAAAMMQPSPGS 120

Query: 121 TVDSSSTDHXXXXXXXXXXXXXXXXXDDDENSFIAPELIDNRYSVCFSPPTFLYGGGSHT 180
           TVDSSSTDH                 DDDENSFIAPELIDNRYSVCFSPPTFLYGGGSHT
Sbjct: 121 TVDSSSTDHGGAGAGGQGAGLQAAALDDDENSFIAPELIDNRYSVCFSPPTFLYGGGSHT 180

Query: 181 YDGDDYGL 188
           YDGDDYGL
Sbjct: 181 YDGDDYGL 188



>Os05g0390800 VQ domain containing protein
          Length = 169

 Score = 68.2 bits (165), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 31/36 (86%), Positives = 33/36 (91%)

Query: 48 IKVVYIANPMRVTTSEEGFRALVQELTGRHADPSKY 83
          IKVVYI+NPMRV TS  GFRALVQELTGR+ADPSKY
Sbjct: 43 IKVVYISNPMRVRTSAAGFRALVQELTGRNADPSKY 78



>Os01g0808900 Similar to SigA binding protein
          Length = 184

 Score = 68.2 bits (165), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 31/36 (86%), Positives = 33/36 (91%)

Query: 48 IKVVYIANPMRVTTSEEGFRALVQELTGRHADPSKY 83
          IKVVYI+NPMRV TS  GFRALVQELTGR+ADPSKY
Sbjct: 39 IKVVYISNPMRVKTSAAGFRALVQELTGRNADPSKY 74



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.134    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,128,134
Number of extensions: 191096
Number of successful extensions: 504
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 501
Number of HSP's successfully gapped: 3
Length of query: 188
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 93
Effective length of database: 12,075,471
Effective search space: 1123018803
Effective search space used: 1123018803
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 153 (63.5 bits)