BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0438600 Os05g0438600|AY035554
         (141 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0438600  Similar to Fructose-1,6-bisphosphatase, cytoso...   292   7e-80
Os01g0866400  Similar to Fructose-1,6-bisphosphatase (EC 3.1...   273   3e-74
Os03g0267300  Similar to Fructose-1,6-bisphosphatase, chloro...   148   1e-36
Os06g0664200  Inositol phosphatase/fructose-1,6-bisphosphata...   117   2e-27

>Os05g0438600 Similar to Fructose-1,6-bisphosphatase, cytosolic (EC 3.1.3.11)
           (D-fructose-1,6- bisphosphate 1-phosphohydrolase)
           (FBPase) (CY-F1)
          Length = 141

 Score =  292 bits (747), Expect = 7e-80,   Method: Compositional matrix adjust.
 Identities = 141/141 (100%), Positives = 141/141 (100%)

Query: 1   IFGIYMIKDKDNVTLDDVLQPGTDMLAAGYCMYGSSCTLVLSTGNGVNGFTLDPSLGEFI 60
           IFGIYMIKDKDNVTLDDVLQPGTDMLAAGYCMYGSSCTLVLSTGNGVNGFTLDPSLGEFI
Sbjct: 1   IFGIYMIKDKDNVTLDDVLQPGTDMLAAGYCMYGSSCTLVLSTGNGVNGFTLDPSLGEFI 60

Query: 61  LTHPNIKIPNRGKIYSVNEGNAKNWDAPTAKFVEKCKFPQDGSPSKSLRYIGSMVADVHR 120
           LTHPNIKIPNRGKIYSVNEGNAKNWDAPTAKFVEKCKFPQDGSPSKSLRYIGSMVADVHR
Sbjct: 61  LTHPNIKIPNRGKIYSVNEGNAKNWDAPTAKFVEKCKFPQDGSPSKSLRYIGSMVADVHR 120

Query: 121 TLLYGGIFLYPADQKSPNGKL 141
           TLLYGGIFLYPADQKSPNGKL
Sbjct: 121 TLLYGGIFLYPADQKSPNGKL 141



>Os01g0866400 Similar to Fructose-1,6-bisphosphatase (EC 3.1.3.11) (Fragment)
          Length = 339

 Score =  273 bits (698), Expect = 3e-74,   Method: Compositional matrix adjust.
 Identities = 127/141 (90%), Positives = 136/141 (96%)

Query: 1   IFGIYMIKDKDNVTLDDVLQPGTDMLAAGYCMYGSSCTLVLSTGNGVNGFTLDPSLGEFI 60
           IFGIYMIKDK+NVTL+DVLQPG +M+AAGYCMYGSSCTLVLSTGNGVNGFTLDPSLGEFI
Sbjct: 138 IFGIYMIKDKENVTLEDVLQPGKNMVAAGYCMYGSSCTLVLSTGNGVNGFTLDPSLGEFI 197

Query: 61  LTHPNIKIPNRGKIYSVNEGNAKNWDAPTAKFVEKCKFPQDGSPSKSLRYIGSMVADVHR 120
           LTHP+IKIP +GKIYSVNEGNAKNWD PTAKFVEKCKFP+DGS  KSLRYIGSMVADVHR
Sbjct: 198 LTHPDIKIPKKGKIYSVNEGNAKNWDEPTAKFVEKCKFPKDGSSPKSLRYIGSMVADVHR 257

Query: 121 TLLYGGIFLYPADQKSPNGKL 141
           TLLYGG+FLYPAD+KSPNGKL
Sbjct: 258 TLLYGGVFLYPADKKSPNGKL 278



>Os03g0267300 Similar to Fructose-1,6-bisphosphatase, chloroplast precursor (EC
           3.1.3.11) (D- fructose-1,6-bisphosphate
           1-phosphohydrolase) (FBPase)
          Length = 406

 Score =  148 bits (373), Expect = 1e-36,   Method: Compositional matrix adjust.
 Identities = 79/155 (50%), Positives = 94/155 (60%), Gaps = 14/155 (9%)

Query: 1   IFGIYMIKDK------DNVTLDDV--------LQPGTDMLAAGYCMYGSSCTLVLSTGNG 46
           IFGIY   D+      D+  LD V         QPG+++LAAGYCMY SS   VL+ G G
Sbjct: 195 IFGIYSPNDECLADIADDQNLDQVEQRCIVSVCQPGSNLLAAGYCMYSSSVIFVLTIGTG 254

Query: 47  VNGFTLDPSLGEFILTHPNIKIPNRGKIYSVNEGNAKNWDAPTAKFVEKCKFPQDGSPSK 106
           V  FTLDP  GEF+LT   ++IP  GKIY+ NEGN   WD     +++  K P       
Sbjct: 255 VYVFTLDPMYGEFVLTQEKVQIPKAGKIYAFNEGNYALWDDKLKSYMDSLKEPGPSGKPY 314

Query: 107 SLRYIGSMVADVHRTLLYGGIFLYPADQKSPNGKL 141
           S RYIGS+V D HRTLLYGGI+ YP DQKS NGKL
Sbjct: 315 SARYIGSLVGDFHRTLLYGGIYGYPRDQKSKNGKL 349



>Os06g0664200 Inositol phosphatase/fructose-1,6-bisphosphatase family protein
          Length = 401

 Score =  117 bits (293), Expect = 2e-27,   Method: Compositional matrix adjust.
 Identities = 60/142 (42%), Positives = 88/142 (61%), Gaps = 9/142 (6%)

Query: 1   IFGIY-------MIKDKDNVTLDDVLQPGTDMLAAGYCMYGSSCTLVLSTGNGVNGFTLD 53
           IFGIY        + +++   L+  LQ GT ++A+GY +Y S+    +S G G +GFTLD
Sbjct: 192 IFGIYNRLAELDHLPEEERAQLNS-LQSGTHLVASGYVLYSSATIFCISFGAGTHGFTLD 250

Query: 54  PSLGEFILTHPNIKIPNRGKIYSVNEGNAKNWDAPTAKFVEKCKFPQDGSPSK-SLRYIG 112
              GEF+LTHP+I+IP RG+IYSVN+    +W     K+++  +  +   P K S RY+ 
Sbjct: 251 HLTGEFVLTHPSIQIPPRGQIYSVNDARYFDWPEGLRKYIDTIRQGKGQHPKKYSARYVC 310

Query: 113 SMVADVHRTLLYGGIFLYPADQ 134
           S+VAD HRTL+YGG+ + P D 
Sbjct: 311 SLVADFHRTLIYGGVAMNPRDH 332



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.139    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,414,937
Number of extensions: 227541
Number of successful extensions: 354
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 353
Number of HSP's successfully gapped: 4
Length of query: 141
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 50
Effective length of database: 12,284,327
Effective search space: 614216350
Effective search space used: 614216350
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 151 (62.8 bits)