BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0428400 Os05g0428400|Os05g0428400
         (225 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0428400  UspA domain containing protein                      390   e-109
Os01g0875300  UspA domain containing protein                      256   9e-69
Os12g0501400  UspA domain containing protein                       86   2e-17
Os10g0463300  UspA domain containing protein                       70   1e-12

>Os05g0428400 UspA domain containing protein
          Length = 225

 Score =  390 bits (1003), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 197/225 (87%), Positives = 197/225 (87%)

Query: 1   MGSSGGSSEHFLRQFSASDGAPLPRELGEEWAAAECXXXXXXXXXXXXXXXXXXXXXXXX 60
           MGSSGGSSEHFLRQFSASDGAPLPRELGEEWAAAEC                        
Sbjct: 1   MGSSGGSSEHFLRQFSASDGAPLPRELGEEWAAAECGRRGSRRWSRKKARGHHRRGGGGG 60

Query: 61  XXXXLCRSREEAPAGRKRVMVVVDQSSGAKHAMMWALTHVASKGDFLTLLHVLPHGGGDA 120
               LCRSREEAPAGRKRVMVVVDQSSGAKHAMMWALTHVASKGDFLTLLHVLPHGGGDA
Sbjct: 61  GGGGLCRSREEAPAGRKRVMVVVDQSSGAKHAMMWALTHVASKGDFLTLLHVLPHGGGDA 120

Query: 121 SALANSLGSLCKACKPEVEVEALVIQGPKLGTVLSQVKKLDASVLVLSQCKPSPFCCFMR 180
           SALANSLGSLCKACKPEVEVEALVIQGPKLGTVLSQVKKLDASVLVLSQCKPSPFCCFMR
Sbjct: 121 SALANSLGSLCKACKPEVEVEALVIQGPKLGTVLSQVKKLDASVLVLSQCKPSPFCCFMR 180

Query: 181 SSGEEFVEECINRADCLTLAVRRQSKGVGGYLISTRWQKNFWLLA 225
           SSGEEFVEECINRADCLTLAVRRQSKGVGGYLISTRWQKNFWLLA
Sbjct: 181 SSGEEFVEECINRADCLTLAVRRQSKGVGGYLISTRWQKNFWLLA 225



>Os01g0875300 UspA domain containing protein
          Length = 241

 Score =  256 bits (654), Expect = 9e-69,   Method: Compositional matrix adjust.
 Identities = 127/157 (80%), Positives = 140/157 (89%), Gaps = 7/157 (4%)

Query: 76  RKRVMVVVDQSSGAKHAMMWALTHVASKGDFLTLLHVLPHGGG----DASALANSLGSLC 131
           RKRVMVVVD +SGAKHAMMWALTHVA+KGDFLTLLHVLP+ G     +  +LANSLG+LC
Sbjct: 85  RKRVMVVVDDTSGAKHAMMWALTHVANKGDFLTLLHVLPYAGAGRGEETPSLANSLGTLC 144

Query: 132 KACKPEVEVEALVIQGPKLGTVLSQVKKLDASVLVLSQCKPSPFC---CFMRSSGEEFVE 188
           KAC+PEVEVEALVIQGPKL TVLSQVKKL+ASVLVLSQ KPS FC   C +RSS EEFVE
Sbjct: 145 KACRPEVEVEALVIQGPKLATVLSQVKKLEASVLVLSQSKPSHFCWLSCILRSSSEEFVE 204

Query: 189 ECINRADCLTLAVRRQSKGVGGYLISTRWQKNFWLLA 225
           +CIN+A+CLTLAVR+QSKGVGGYLISTRWQKNFWLLA
Sbjct: 205 QCINQAECLTLAVRKQSKGVGGYLISTRWQKNFWLLA 241



>Os12g0501400 UspA domain containing protein
          Length = 225

 Score = 85.9 bits (211), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 63/176 (35%), Positives = 91/176 (51%), Gaps = 21/176 (11%)

Query: 70  EEAPAGRKRVMVVVDQSSG-AKHAMMWALTHVASKGDFLTLLHVLPHGGGDASALA---- 124
           E++  GRK VMVV D  S  A+ A+ WAL+H     D + LL V+  GG           
Sbjct: 51  EKSEVGRK-VMVVADGGSDEARTALQWALSHSVRPCDTVVLLDVVKSGGDGGGKNGDDPS 109

Query: 125 ------NSLGSLCKACKPEVEVEALVIQGPKLG-TVLSQVKKLDASVLVLSQCKPSP--- 174
                  ++ S+C+A +PEV VE  +++G + G  ++   +K   S+LV+ Q K S    
Sbjct: 110 RGCQHLETMRSICQAKRPEVRVELSLVEGKERGPAIVEAARKQGVSLLVMGQKKRSITWR 169

Query: 175 -----FCCFMRSSGEEFVEECINRADCLTLAVRRQSKGVGGYLISTRWQKNFWLLA 225
                        G    E C+  A C+ LAVRR+S+  GGYLI+TR Q++FWLLA
Sbjct: 170 LLVMWMTGGKGGGGRGTAEYCVQNAACMALAVRRKSRRGGGYLITTRRQRDFWLLA 225



>Os10g0463300 UspA domain containing protein
          Length = 206

 Score = 70.5 bits (171), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 54/193 (27%), Positives = 87/193 (45%), Gaps = 40/193 (20%)

Query: 72  APAGRKRVMVVVDQSSGAKHAMMWALTHVASKGDFLTLLHV-LP-------------HGG 117
           AP   + VM++ D    +  AM WAL+H  ++GD + LLH+ +P              GG
Sbjct: 15  APGKPRLVMIIADPGRESTAAMEWALSHAVAEGDAILLLHINMPPNSAGGAGPSRTGSGG 74

Query: 118 GDASALANSLG--------------SLCKACKPEVEVEALVIQGPKLG------TVLSQV 157
              S L   LG              + CKA  P   V AL ++    G      T+L++ 
Sbjct: 75  SAGSPLTALLGAGAAGDADFMETMSAACKARHPRARVRALRVEPATEGREAKAQTILAES 134

Query: 158 KKLDASVLVLSQCKPSPFCCFMRSSG-----EEFVEECINRADCLTLAVRRQSKGVGGYL 212
           ++    +LV+   + S F      SG     +   E  I  + C+ ++V+++ +   GYL
Sbjct: 135 QRRGVELLVIGHRRVSSFLGLRSPSGSSRAHDSTAEFLIEHSKCVCVSVQKKGQNA-GYL 193

Query: 213 ISTRWQKNFWLLA 225
           ++T+  KNFWLLA
Sbjct: 194 LNTKTHKNFWLLA 206



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.132    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,564,931
Number of extensions: 268502
Number of successful extensions: 706
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 706
Number of HSP's successfully gapped: 4
Length of query: 225
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 128
Effective length of database: 11,971,043
Effective search space: 1532293504
Effective search space used: 1532293504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 154 (63.9 bits)