BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0393700 Os05g0393700|009-005-F10
         (262 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0393700  Conserved hypothetical protein                      538   e-153
Os09g0469400  Similar to Isoamylase-type starch debranching ...   107   7e-24
Os08g0520900  Similar to Isoamylase (Fragment)                    107   8e-24

>Os05g0393700 Conserved hypothetical protein
          Length = 262

 Score =  538 bits (1386), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 262/262 (100%), Positives = 262/262 (100%)

Query: 1   LATRLCGSGDLFSTRGPAFSFNHVSRNSGLSLVDLVSFSNDDLLSESSWNCGEEGPSENS 60
           LATRLCGSGDLFSTRGPAFSFNHVSRNSGLSLVDLVSFSNDDLLSESSWNCGEEGPSENS
Sbjct: 1   LATRLCGSGDLFSTRGPAFSFNHVSRNSGLSLVDLVSFSNDDLLSESSWNCGEEGPSENS 60

Query: 61  AVLQTRLRQIRNFLFILFVSLGVPVLNMGDECGHSAAGSVSYKDRGPLNWRGMKTTFVKE 120
           AVLQTRLRQIRNFLFILFVSLGVPVLNMGDECGHSAAGSVSYKDRGPLNWRGMKTTFVKE
Sbjct: 61  AVLQTRLRQIRNFLFILFVSLGVPVLNMGDECGHSAAGSVSYKDRGPLNWRGMKTTFVKE 120

Query: 121 VTGFISFLTALRSRRGDIFQRREFLKLENIHWYGSDLCEPGWDDPTSNFLCMHINAEVDE 180
           VTGFISFLTALRSRRGDIFQRREFLKLENIHWYGSDLCEPGWDDPTSNFLCMHINAEVDE
Sbjct: 121 VTGFISFLTALRSRRGDIFQRREFLKLENIHWYGSDLCEPGWDDPTSNFLCMHINAEVDE 180

Query: 181 MAADSVRGDLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVP 240
           MAADSVRGDLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVP
Sbjct: 181 MAADSVRGDLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVP 240

Query: 241 GLSSYHVEAHTCVLFESKSALA 262
           GLSSYHVEAHTCVLFESKSALA
Sbjct: 241 GLSSYHVEAHTCVLFESKSALA 262



>Os09g0469400 Similar to Isoamylase-type starch debranching enzyme ISO3
          Length = 377

 Score =  107 bits (268), Expect = 7e-24,   Method: Compositional matrix adjust.
 Identities = 81/273 (29%), Positives = 126/273 (46%), Gaps = 32/273 (11%)

Query: 1   LATRLCGSGDLFST--RGPAFSFNHVSRNSGLSLVDLVSFS--NDDLLSES--------- 47
            ATR+ GS DL+    R P    N V  + G +L DLVS++  ++D   E          
Sbjct: 121 FATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNF 180

Query: 48  SWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNMGDECGHSAAG-SVSYKDRG 106
           SWNCG EG + +  VL  R RQ++NF   L +S G P++ MGDE GH+  G + SY    
Sbjct: 181 SWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDT 240

Query: 107 PLNWRGMKTTFVKEVTGFISFLTALRSRRGD-IFQRREFLKLENIHWYGSDLCEPGWDDP 165
            +N    +    +    F  F   ++ R  + I +R  FL   ++ W+     E  W++ 
Sbjct: 241 CINNFQWEQLEQRRDGHFRFFSEMIKFRHSNPILRRDRFLNKNDVTWH-----EDCWENQ 295

Query: 166 TSNFLCMHINAEVDEMAADSVRGDLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFP 225
            S FL   ++         +  GD+Y+ FNA++  V A +P       W R+VDT+L  P
Sbjct: 296 ESKFLAFTVHDH-------NSGGDIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESP 348

Query: 226 GFFATESNPKVQQVPGLSSYHVEAHTCVLFESK 258
                E  P          Y +  ++ +L ++K
Sbjct: 349 NDIVPEGVPFTG-----PKYRIAPYSSILLKAK 376



>Os08g0520900 Similar to Isoamylase (Fragment)
          Length = 725

 Score =  107 bits (267), Expect = 8e-24,   Method: Compositional matrix adjust.
 Identities = 81/249 (32%), Positives = 117/249 (46%), Gaps = 26/249 (10%)

Query: 1   LATRLCGSGDLFST--RGPAFSFNHVSRNSGLSLVDLVSFSN--------DDLLSES--- 47
            A  LCGS  L+    R P  S N V  + G +L DLV+++         D+   E+   
Sbjct: 454 FAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKKYNSSNGEDNRDGENHNL 513

Query: 48  SWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNMGDECGHSAAG-SVSYKDRG 106
           SWNCGEEG     +V + R RQ+RNF   L VS GVP+  MGDE GH+  G + +Y    
Sbjct: 514 SWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDH 573

Query: 107 PLNW-RGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLENIHWYGSDLCEPGWDDP 165
            +N+ R  K     ++  F S +T  R ++ +     +F   + +HW+G    +P W + 
Sbjct: 574 YVNYFRWDKKEESSDLQRFCSLMTKFR-KQCESLGLADFPTAQRLHWHGHQPGKPDWSE- 631

Query: 166 TSNFLCMHINAEVDEMAADSVRGDLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFP 225
           TS F+             D  +G++Y+ FNA+       LP    G  W  LVDT    P
Sbjct: 632 TSRFVAFS--------TKDETKGEIYVAFNASHLPAVVGLPE-RPGYRWEPLVDTGKPAP 682

Query: 226 GFFATESNP 234
             F T+  P
Sbjct: 683 YDFLTDDLP 691



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.135    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,771,468
Number of extensions: 342056
Number of successful extensions: 832
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 829
Number of HSP's successfully gapped: 3
Length of query: 262
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 163
Effective length of database: 11,866,615
Effective search space: 1934258245
Effective search space used: 1934258245
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 155 (64.3 bits)