BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0390800 Os05g0390800|Os05g0390800
         (169 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0390800  VQ domain containing protein                        186   9e-48
Os01g0808900  Similar to SigA binding protein                     102   2e-22
Os05g0491700  VQ domain containing protein                         68   4e-12

>Os05g0390800 VQ domain containing protein
          Length = 169

 Score =  186 bits (471), Expect = 9e-48,   Method: Compositional matrix adjust.
 Identities = 102/169 (60%), Positives = 102/169 (60%)

Query: 1   MDRQRQQSSRGNASATATRXXXXXXXXXXXXXXXXXXXXXXPIKVVYISNPMRVRTSAAG 60
           MDRQRQQSSRGNASATATR                      PIKVVYISNPMRVRTSAAG
Sbjct: 1   MDRQRQQSSRGNASATATRGGGGGGGGKGGGNGKAAAAGKKPIKVVYISNPMRVRTSAAG 60

Query: 61  FRALVQELTGRNADPSKYSPRASXXXXXXXXXXXXXXXXXXXXXXXXXXXXPGRHPAETA 120
           FRALVQELTGRNADPSKYSPRAS                            PGRHPAETA
Sbjct: 61  FRALVQELTGRNADPSKYSPRASAGDDGGGATALPDTGAASDADALEAGAAPGRHPAETA 120

Query: 121 TFXXXXXXXXXXXXXXXXXVFRSQLLDTSYSVFSPPTLLYDDHPHSKSN 169
           TF                 VFRSQLLDTSYSVFSPPTLLYDDHPHSKSN
Sbjct: 121 TFDEGGGGGGGGYDDDDDDVFRSQLLDTSYSVFSPPTLLYDDHPHSKSN 169



>Os01g0808900 Similar to SigA binding protein
          Length = 184

 Score =  102 bits (253), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 56/83 (67%), Positives = 58/83 (69%), Gaps = 4/83 (4%)

Query: 1  MDRQRQQSSRGNASATATRXXXXXXXXXXXXXXXXXXXXXXPIKVVYISNPMRVRTSAAG 60
          MDRQRQQSSRGNA+AT                         PIKVVYISNPMRV+TSAAG
Sbjct: 1  MDRQRQQSSRGNATAT----RGGGSSGKGGGGGVGKAAGKKPIKVVYISNPMRVKTSAAG 56

Query: 61 FRALVQELTGRNADPSKYSPRAS 83
          FRALVQELTGRNADPSKYSPRAS
Sbjct: 57 FRALVQELTGRNADPSKYSPRAS 79



>Os05g0491700 VQ domain containing protein
          Length = 188

 Score = 67.8 bits (164), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 31/36 (86%), Positives = 33/36 (91%)

Query: 43 IKVVYISNPMRVRTSAAGFRALVQELTGRNADPSKY 78
          IKVVYI+NPMRV TS  GFRALVQELTGR+ADPSKY
Sbjct: 48 IKVVYIANPMRVTTSEEGFRALVQELTGRHADPSKY 83



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.126    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,235,317
Number of extensions: 57082
Number of successful extensions: 113
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 109
Number of HSP's successfully gapped: 3
Length of query: 169
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 75
Effective length of database: 12,127,685
Effective search space: 909576375
Effective search space used: 909576375
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 152 (63.2 bits)