BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0149000 Os05g0149000|AK111047
         (183 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0149000  Pectinesterase inhibitor domain containing pro...   259   5e-70
Os02g0537000  Pectinesterase inhibitor domain containing pro...    97   5e-21
Os03g0100100  Pectinesterase inhibitor domain containing pro...    72   2e-13

>Os05g0149000 Pectinesterase inhibitor domain containing protein
          Length = 183

 Score =  259 bits (663), Expect = 5e-70,   Method: Compositional matrix adjust.
 Identities = 135/165 (81%), Positives = 135/165 (81%)

Query: 19  CSGRAPVAEATIESTCGAAAKGDRRVDVGFCARQFASYHGAAEADAWGLAKIAALIGVNL 78
           CSGRAPVAEATIESTCGAAAKGDRRVDVGFCARQFASYHGAAEADAWGLAKIAALIGVNL
Sbjct: 19  CSGRAPVAEATIESTCGAAAKGDRRVDVGFCARQFASYHGAAEADAWGLAKIAALIGVNL 78

Query: 79  ADDAVFDIGAGKIRPSPXXXXXXXXXXXXXXXXYDAVGVAFAEAADELGSRRYXXXXXXX 138
           ADDAVFDIGAGKIRPSP                YDAVGVAFAEAADELGSRRY       
Sbjct: 79  ADDAVFDIGAGKIRPSPGGGAKGDKAMDACAKAYDAVGVAFAEAADELGSRRYAAARQEL 138

Query: 139 XXXXXXXQRCDGGLSRAGARSPLPRYSADCQQVAIMGIAITNLLK 183
                  QRCDGGLSRAGARSPLPRYSADCQQVAIMGIAITNLLK
Sbjct: 139 ARVAALVQRCDGGLSRAGARSPLPRYSADCQQVAIMGIAITNLLK 183



>Os02g0537000 Pectinesterase inhibitor domain containing protein
          Length = 183

 Score = 97.4 bits (241), Expect = 5e-21,   Method: Compositional matrix adjust.
 Identities = 61/157 (38%), Positives = 83/157 (52%), Gaps = 4/157 (2%)

Query: 28  ATIESTCGAAAKGDRRVDVGFCARQFASYHGAAEADAWGLAKIAALIGVNLADDAVFDIG 87
           AT E+ C AAA+ DRRVD  FC  + + +H + +AD WGLAK+AA +GV +A DA +D  
Sbjct: 30  ATPETACRAAAEEDRRVDYEFCVSRLSHHHDSPDADTWGLAKVAADVGVCIAGDAAYDAK 89

Query: 88  AGKIRPSPXXXXXXXXXXXXXXXXYDAVGVAFAEAADELGSRRYXXXXXXXXXXXXXXQR 147
           A                       YD +G AFA A D++  R Y              +R
Sbjct: 90  AKL---QAAKAGGEREALERCAELYDRMGSAFAAAYDDINRREYAAGKEKAGEAASLARR 146

Query: 148 CDGGLSRAG-ARSPLPRYSADCQQVAIMGIAITNLLK 183
           CDG  + AG A SPL R +A+  ++AI+  AITNL+K
Sbjct: 147 CDGAFADAGVAPSPLERQTAESVKIAIVCTAITNLVK 183



>Os03g0100100 Pectinesterase inhibitor domain containing protein
          Length = 203

 Score = 72.0 bits (175), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 51/150 (34%), Positives = 70/150 (46%), Gaps = 22/150 (14%)

Query: 27  EATIESTCGAAAKGDRRVDVGFCARQFASYHGAAEADAWGLAKIAALIGVNLADDAVFDI 86
           +AT+ESTC  AA  D+RV +  C  Q   +    +ADAWGLAK A L+GV+ AD A  DI
Sbjct: 25  DATVESTCSDAAASDKRVHLAMCLSQLGHHR---DADAWGLAKAATLVGVDKADLAADDI 81

Query: 87  -----GAGK--IRPSPXXXXXXXXXXXXXXXXYDAVGVAFAEAADELGSRRYXXXXXXXX 139
                GA    I+P+                 Y  VG AFA A D + +R Y        
Sbjct: 82  KELEAGASTAGIKPA----------LAECAKQYRGVGFAFASAHDVINNRAYDVGEKKLD 131

Query: 140 XXXXXXQRCDGGLSRAGA--RSPLPRYSAD 167
                 Q+C+   ++ G   + PL + +AD
Sbjct: 132 EALSLTQKCNAAFAKIGVPLQQPLAQLTAD 161



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.134    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,492,040
Number of extensions: 147884
Number of successful extensions: 269
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 262
Number of HSP's successfully gapped: 4
Length of query: 183
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 88
Effective length of database: 12,075,471
Effective search space: 1062641448
Effective search space used: 1062641448
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 153 (63.5 bits)