BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0623600 Os04g0623600|AK068129
(62 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os04g0623600 Similar to (S)-2-hydroxy-acid oxidase, peroxis... 132 8e-32
Os04g0623500 Similar to (S)-2-hydroxy-acid oxidase, peroxis... 90 3e-19
Os03g0786100 Similar to Glycolate oxidase (EC 1.1.3.15) (Fr... 76 5e-15
Os07g0152900 Similar to Glycolate oxidase (EC 1.1.3.15) (Fr... 75 1e-14
>Os04g0623600 Similar to (S)-2-hydroxy-acid oxidase, peroxisomal (EC 1.1.3.15)
(Glycolate oxidase) (GOX) (Short chain alpha-hydroxy
acid oxidase)
Length = 62
Score = 132 bits (331), Expect = 8e-32, Method: Compositional matrix adjust.
Identities = 62/62 (100%), Positives = 62/62 (100%)
Query: 1 MKLVGLRLPGPNCREMALVTNVCEYEELAKHKLPKMVYDFYAVDAEDQWTLRENSEAFSR 60
MKLVGLRLPGPNCREMALVTNVCEYEELAKHKLPKMVYDFYAVDAEDQWTLRENSEAFSR
Sbjct: 1 MKLVGLRLPGPNCREMALVTNVCEYEELAKHKLPKMVYDFYAVDAEDQWTLRENSEAFSR 60
Query: 61 IL 62
IL
Sbjct: 61 IL 62
>Os04g0623500 Similar to (S)-2-hydroxy-acid oxidase, peroxisomal (EC 1.1.3.15)
(Glycolate oxidase) (GOX) (Short chain alpha-hydroxy
acid oxidase)
Length = 389
Score = 90.1 bits (222), Expect = 3e-19, Method: Composition-based stats.
Identities = 39/55 (70%), Positives = 45/55 (81%)
Query: 8 LPGPNCREMALVTNVCEYEELAKHKLPKMVYDFYAVDAEDQWTLRENSEAFSRIL 62
+P P EM L+TNV EYE+LAK KLPKM+YD+YA AEDQWTL+EN EAFSRIL
Sbjct: 15 IPLPETEEMELITNVSEYEQLAKQKLPKMIYDYYASGAEDQWTLKENREAFSRIL 69
>Os03g0786100 Similar to Glycolate oxidase (EC 1.1.3.15) (Fragment)
Length = 369
Score = 75.9 bits (185), Expect = 5e-15, Method: Composition-based stats.
Identities = 32/47 (68%), Positives = 39/47 (82%)
Query: 16 MALVTNVCEYEELAKHKLPKMVYDFYAVDAEDQWTLRENSEAFSRIL 62
M +TNV EY+ +AK KLPKM+YD+YA AED+WTL+EN EAFSRIL
Sbjct: 1 MGEITNVMEYQAIAKQKLPKMIYDYYASGAEDEWTLKENREAFSRIL 47
>Os07g0152900 Similar to Glycolate oxidase (EC 1.1.3.15) (Fragment)
Length = 369
Score = 75.1 bits (183), Expect = 1e-14, Method: Composition-based stats.
Identities = 31/47 (65%), Positives = 39/47 (82%)
Query: 16 MALVTNVCEYEELAKHKLPKMVYDFYAVDAEDQWTLRENSEAFSRIL 62
M +TNV EY+ +AK KLPKM+YD+YA AED+WTL+EN EAF+RIL
Sbjct: 1 MGEITNVTEYQAIAKQKLPKMIYDYYASGAEDEWTLQENREAFARIL 47
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.321 0.136 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,102,857
Number of extensions: 59889
Number of successful extensions: 135
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 135
Number of HSP's successfully gapped: 4
Length of query: 62
Length of database: 17,035,801
Length adjustment: 34
Effective length of query: 28
Effective length of database: 15,260,525
Effective search space: 427294700
Effective search space used: 427294700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)