BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0620600 Os04g0620600|AK121449
         (92 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0620600  Similar to NOI protein                              191   1e-49
Os09g0253000  Similar to NOI protein                               64   2e-11
Os02g0725400  Nitrate-induced NOI family protein                   63   6e-11

>Os04g0620600 Similar to NOI protein
          Length = 92

 Score =  191 bits (485), Expect = 1e-49,   Method: Compositional matrix adjust.
 Identities = 92/92 (100%), Positives = 92/92 (100%)

Query: 1  MTTMDKGRALPKFGEWDVKNPASAEGFTVIFQKARDDKKTTGPGNARVGIPPAFRSTDDD 60
          MTTMDKGRALPKFGEWDVKNPASAEGFTVIFQKARDDKKTTGPGNARVGIPPAFRSTDDD
Sbjct: 1  MTTMDKGRALPKFGEWDVKNPASAEGFTVIFQKARDDKKTTGPGNARVGIPPAFRSTDDD 60

Query: 61 GGYRPEFKPAESYQQHTPPKRVKKKWAFCAGC 92
          GGYRPEFKPAESYQQHTPPKRVKKKWAFCAGC
Sbjct: 61 GGYRPEFKPAESYQQHTPPKRVKKKWAFCAGC 92



>Os09g0253000 Similar to NOI protein
          Length = 82

 Score = 64.3 bits (155), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 30/38 (78%), Positives = 33/38 (86%)

Query: 5  DKGRALPKFGEWDVKNPASAEGFTVIFQKARDDKKTTG 42
          +KG  LPKFGEWDV +PASAEGFTVIF KARD+KKT G
Sbjct: 4  EKGSPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGG 41



>Os02g0725400 Nitrate-induced NOI family protein
          Length = 100

 Score = 62.8 bits (151), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 39/89 (43%), Positives = 47/89 (52%), Gaps = 10/89 (11%)

Query: 5  DKGRALPKFGEWDVKNPASAEGFTVIFQKARDDKKTTGPGNARVGIPPAFRSTDD--DGG 62
          + GR +PKFG WDV NPASA+GFTVIF KARD+KK  GP    V +  + RS  D  D  
Sbjct: 14 ETGRTIPKFGAWDVNNPASADGFTVIFSKARDEKK--GP----VNVDASTRSNSDMKDSN 67

Query: 63 YRPEFKPAESYQQHTPPKRVKKKWAFCAG 91
               +    Y + T      KKW  C  
Sbjct: 68 NNKATEKINPYHRRT--NSASKKWFCCVS 94



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.135    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,219,253
Number of extensions: 178487
Number of successful extensions: 345
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 345
Number of HSP's successfully gapped: 3
Length of query: 92
Length of database: 17,035,801
Length adjustment: 61
Effective length of query: 31
Effective length of database: 13,850,747
Effective search space: 429373157
Effective search space used: 429373157
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 149 (62.0 bits)